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Literature summary extracted from

  • Jung, J.; Kim, J.K.; Yeom, S.J.; Ahn, Y.J.; Oh, D.K.; Kang, L.W.
    Crystal structure of Clostridium thermocellum ribose-5-phosphate isomerase B reveals properties critical for fast enzyme kinetics (2011), Appl. Microbiol. Biotechnol., 90, 517-527.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.6 expressed in Escherichia coli ER2566 cells Acetivibrio thermocellus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.1.6 in complex with ribose 5-phosphate, ribose or allose, sitting drop vapor diffusion method, using 0.05 M Tris pH 7.0, 10% (w/v) PEG 8000, 0.15 M magnesium chloride, and 0.2 M potassium chloride Acetivibrio thermocellus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.6 17000
-
 2 * 17000, X-ray crystallography Acetivibrio thermocellus

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.6 Acetivibrio thermocellus A3DIL8
-
-
5.3.1.6 Acetivibrio thermocellus ATCC 27405 A3DIL8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.6 HisTrap HP column chromatography Acetivibrio thermocellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.6 D-allose
-
 Acetivibrio thermocellus D-psicose
-
 ?
5.3.1.6 D-allose
-
 Acetivibrio thermocellus ATCC 27405 D-psicose
-
 ?
5.3.1.6 D-ribose
-
 Acetivibrio thermocellus D-ribulose
-
 ?
5.3.1.6 D-ribose
-
 Acetivibrio thermocellus ATCC 27405 D-ribulose
-
 ?
5.3.1.6 D-ribose 5-phosphate
-
 Acetivibrio thermocellus D-ribulose 5-phosphate
-
 ?
5.3.1.6 D-ribose 5-phosphate
-
 Acetivibrio thermocellus ATCC 27405 D-ribulose 5-phosphate
-
 ?
5.3.1.6 D-talose
-
 Acetivibrio thermocellus D-tagatose
-
 ?
5.3.1.6 D-talose
-
 Acetivibrio thermocellus ATCC 27405 D-tagatose
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.6 homodimer 2 * 17000, X-ray crystallography Acetivibrio thermocellus

Synonyms

EC Number Synonyms Comment Organism
5.3.1.6 ribose-5-phosphate isomerase B
-
 Acetivibrio thermocellus
5.3.1.6 RPI
-
 Acetivibrio thermocellus