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Literature summary extracted from

  • Sakamoto, T.; Ogura, A.; Inui, M.; Tokuda, S.; Hosokawa, S.; Ihara, H.; Kasai, N.
    Identification of a GH62 alpha-L-arabinofuranosidase specific for arabinoxylan produced by Penicillium chrysogenum (2011), Appl. Microbiol. Biotechnol., 90, 137-146.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.55 expressed in Escherichia coli BL21(DE3) cells Penicillium chrysogenum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.55 32827
-
 x * 32827, calculated from amino acid sequence Penicillium chrysogenum
3.2.1.55 35000
-
 gel filtration Penicillium chrysogenum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Penicillium chrysogenum B5MGR2
-
-
3.2.1.55 Penicillium chrysogenum 31B B5MGR2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55 ammonium sulfate precipitation, Mono Q column chromatography, and Mono S column chromatography. Ni-Sepharose 6 column chromatography Penicillium chrysogenum

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Penicillium chrysogenum 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Penicillium chrysogenum 31B 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.55 insoluble wheat arabinoxylan + H2O about 28% activity compared to soluble wheat arabinoxylan, the enzyme is specific for alpha-L-arabinofuranosyl side-chains bound to xylan backbones Penicillium chrysogenum L-arabinose L-arabinose is detected as the only product ?
3.2.1.55 insoluble wheat arabinoxylan + H2O about 28% activity compared to soluble wheat arabinoxylan, the enzyme is specific for alpha-L-arabinofuranosyl side-chains bound to xylan backbones Penicillium chrysogenum 31B L-arabinose L-arabinose is detected as the only product ?
3.2.1.55 additional information 4-nitrophenyl beta-D-xylopyranoside, alpha-1,5-arabinofuranobiose, sugar beet L-arabinan, and birch wood xylan are resistant to the action of recombinant enzyme even when the enzyme is present in excess Penicillium chrysogenum ?
-
 ?
3.2.1.55 additional information no activity with branched sugar beet arabinan Penicillium chrysogenum ?
-
 ?
3.2.1.55 additional information 4-nitrophenyl beta-D-xylopyranoside, alpha-1,5-arabinofuranobiose, sugar beet L-arabinan, and birch wood xylan are resistant to the action of recombinant enzyme even when the enzyme is present in excess Penicillium chrysogenum 31B ?
-
 ?
3.2.1.55 additional information no activity with branched sugar beet arabinan Penicillium chrysogenum 31B ?
-
 ?
3.2.1.55 soluble wheat arabinoxylan + H2O best substrate, the enzyme is specific for alpha-L-arabinofuranosyl side-chains bound to xylan backbones Penicillium chrysogenum L-arabinose L-arabinose is detected as the only product ?
3.2.1.55 soluble wheat arabinoxylan + H2O best substrate, the enzyme is specific for alpha-L-arabinofuranosyl side-chains bound to xylan backbones Penicillium chrysogenum 31B L-arabinose L-arabinose is detected as the only product ?

Subunits

EC Number Subunits Comment Organism
3.2.1.55 ? x * 32827, calculated from amino acid sequence Penicillium chrysogenum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.55 AFase
-
 Penicillium chrysogenum
3.2.1.55 AFS1
-
 Penicillium chrysogenum
3.2.1.55 alpha-L-arabinofuranosidase
-
 Penicillium chrysogenum
3.2.1.55 arabinoxylan arabinofuranohydrolase
-
 Penicillium chrysogenum
3.2.1.55 AXS5
-
 Penicillium chrysogenum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.55 40
-
-
 Penicillium chrysogenum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.55 20 60
-
 Penicillium chrysogenum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.55 40 60 after 1 h pre-incubation of the enzyme in 20 mM Na-acetate buffer (pH 5.0), the enzyme is stable up to 40°C and loses all activity by treatment at 60°C Penicillium chrysogenum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 5
-
-
 Penicillium chrysogenum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.55 4 8
-
 Penicillium chrysogenum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.55 Penicillium chrysogenum calculated from amino acid sequence
-
 4.7

Expression

EC Number Organism Comment Expression
3.2.1.55 Penicillium chrysogenum expression of the axs5 gene is strongly induced by adding D-xylose and arabinoxylan to the culture medium up

General Information

EC Number General Information Comment Organism
3.2.1.55 physiological function arabinoxylan arabinofuranohydrolase AXS5 is involved in xylan degradation Penicillium chrysogenum