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Literature summary extracted from

  • Prabhu, P.; Doan, T.T.; Jeya, M.; Kang, L.W.; Lee, J.K.
    Cloning and characterization of a rhamnose isomerase from Bacillus halodurans (2011), Appl. Microbiol. Biotechnol., 89, 635-644.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.14 expressed in Escherichia coli BL21(DE3) cells Halalkalibacterium halodurans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.14 119
-
L-Mannose in 50 mM malate buffer, at pH 7.0 and 70°C Halalkalibacterium halodurans
5.3.1.14 528
-
L-rhamnose in 50 mM malate buffer, at pH 7.0 and 70°C Halalkalibacterium halodurans
5.3.1.14 771
-
L-Lyxose in 50 mM malate buffer, at pH 7.0 and 70°C Halalkalibacterium halodurans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.14 Co2+ required for catalytic activity, 93% activity at 1 mM compared to Mn2+ Halalkalibacterium halodurans
5.3.1.14 Mg2+ required for catalytic activity, 57% activity at 1 mM compared to Mn2+ Halalkalibacterium halodurans
5.3.1.14 Mn2+ required for catalytic activity, 100% activity at 1 mM Halalkalibacterium halodurans
5.3.1.14 additional information enzyme without any metal ions shows a negligible activity. The enzyme activity is not stimulated by Ca2+, Ba2+, Na2+, Fe2+, or Cu2+ Halalkalibacterium halodurans
5.3.1.14 Zn2+ required for catalytic activity, 20% activity at 1 mM compared to Mn2+ Halalkalibacterium halodurans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.14 48000
-
2 * 48000, SDS-PAGE Halalkalibacterium halodurans
5.3.1.14 48178
-
2 * 48178, calculated from amino acid sequence Halalkalibacterium halodurans
5.3.1.14 121000
-
gel filtration Halalkalibacterium halodurans

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.14 Halalkalibacterium halodurans
-
-
-
5.3.1.14 Halalkalibacterium halodurans ATCC BAA-125
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.14 Ni-NTA column chromatography Halalkalibacterium halodurans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.14 D-gulose 5% activity compared to L-rhamnose Halalkalibacterium halodurans D-sorbose
-
?
5.3.1.14 D-gulose 5% activity compared to L-rhamnose Halalkalibacterium halodurans ATCC BAA-125 D-sorbose
-
?
5.3.1.14 L-lyxose 144% activity compared to L-rhamnose Halalkalibacterium halodurans L-xylulose
-
?
5.3.1.14 L-lyxose 144% activity compared to L-rhamnose Halalkalibacterium halodurans ATCC BAA-125 L-xylulose
-
?
5.3.1.14 L-Mannose 59% activity compared to L-rhamnose Halalkalibacterium halodurans L-Fructose
-
?
5.3.1.14 L-Mannose 59% activity compared to L-rhamnose Halalkalibacterium halodurans ATCC BAA-125 L-Fructose
-
?
5.3.1.14 L-Rhamnose 100% activity Halalkalibacterium halodurans L-Rhamnulose
-
r
5.3.1.14 L-Rhamnose 100% activity Halalkalibacterium halodurans ATCC BAA-125 L-Rhamnulose
-
r
5.3.1.14 L-talose 3% activity compared to L-rhamnose Halalkalibacterium halodurans ?
-
?
5.3.1.14 L-talose 3% activity compared to L-rhamnose Halalkalibacterium halodurans ATCC BAA-125 ?
-
?

Subunits

EC Number Subunits Comment Organism
5.3.1.14 homodimer 2 * 48000, SDS-PAGE Halalkalibacterium halodurans
5.3.1.14 homodimer 2 * 48178, calculated from amino acid sequence Halalkalibacterium halodurans

Synonyms

EC Number Synonyms Comment Organism
5.3.1.14 L-RhI
-
Halalkalibacterium halodurans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.14 70
-
-
Halalkalibacterium halodurans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.14 60 80 the enzyme shows 100% activity for 10 h at 60°C, the half-life of the enzyme is more than 900 min, about 25 min, and 5 min at 60°C, 70°C, and 80°C, respectively Halalkalibacterium halodurans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.14 88.88
-
L-Mannose in 50 mM malate buffer, at pH 7.0 and 70°C Halalkalibacterium halodurans
5.3.1.14 149.5
-
L-rhamnose in 50 mM malate buffer, at pH 7.0 and 70°C Halalkalibacterium halodurans
5.3.1.14 216
-
L-Lyxose in 50 mM malate buffer, at pH 7.0 and 70°C Halalkalibacterium halodurans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.14 7
-
-
Halalkalibacterium halodurans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.1.14 6 10 the optimum pH for isomerization is pH 7.0, with 75%, 85%, 92%, and 90% of maximum activity at pH 6.0, 8.0, 9.0 and 10.0, respectively Halalkalibacterium halodurans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
5.3.1.14 Halalkalibacterium halodurans isoelectric focusing
-
5.5

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.1.14 0.283
-
L-rhamnose in 50 mM malate buffer, at pH 7.0 and 70°C Halalkalibacterium halodurans