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Literature summary extracted from

  • Hua, C.; Yan, Q.; Jiang, Z.; Li, Y.; Katrolia, P.
    High-level expression of a specific beta-1,3-1,4-glucanase from the thermophilic fungus Paecilomyces thermophila in Pichia pastoris (2010), Appl. Microbiol. Biotechnol., 88, 509-518.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.73 synthesis upon expression in Pichia pastoirs as active extracellular beta-1,3-1,4-glucanase, the recombinant protein is secreted predominantly into the medium and comprises up to 85% of the total extracellular proteins and reaches a protein concentration of 9.1 g/l with an activity of 55,300 U/ml in 5-l fermentor culture Paecilomyces sp. 'thermophila'

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.73 expression in Pichia pastoris Paecilomyces sp. 'thermophila'

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.73 31923
-
 x * 38500, SDS-PAGE, x * 31923, calculated Paecilomyces sp. 'thermophila'
3.2.1.73 38500
-
 x * 38500, SDS-PAGE, x * 31923, calculated Paecilomyces sp. 'thermophila'

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.73 Paecilomyces sp. 'thermophila' E0XN39
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.73 no glycoprotein no N-glycosylation sites present in the protein, Endo H treatment does not result in a protein band shift Paecilomyces sp. 'thermophila'

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.73 8208
-
 substrate lichenan, pH 7.0, 70°C Paecilomyces sp. 'thermophila'
3.2.1.73 11940
-
 substrate barley beta-glucan, pH 7.0, 70°C Paecilomyces sp. 'thermophila'

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.73 beta-1,3-1,4-glucan + H2O source of substrate: barley Paecilomyces sp. 'thermophila' ?
-
 ?
3.2.1.73 lichenan + H2O 68.8% of the activity with barley beta-glucan Paecilomyces sp. 'thermophila' ?
-
 ?
3.2.1.73 additional information strictly specific for beta-1,3-1,4-glucans. No substrates are: Avicel, carboxymethyl-cellulose, cellulose, filter paper, beta-1,3-glucan, laminarin, and birchwood xylan Paecilomyces sp. 'thermophila' ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.73 ? x * 38500, SDS-PAGE, x * 31923, calculated Paecilomyces sp. 'thermophila'

Synonyms

EC Number Synonyms Comment Organism
3.2.1.73 Lic16A
-
 Paecilomyces sp. 'thermophila'

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.73 70
-
-
 Paecilomyces sp. 'thermophila'

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.73 50
-
 30 min, more than 90% of maximum activity Paecilomyces sp. 'thermophila'
3.2.1.73 70
-
 30 min, 65% of maximum activity Paecilomyces sp. 'thermophila'

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.73 7
-
-
 Paecilomyces sp. 'thermophila'