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Literature summary extracted from

  • Dueckers, N.; Baer, K.; Simon, S.; Groeger, H.; Hummel, W.
    Threonine aldolases-screening, properties and applications in the synthesis of non-proteinogenic beta-hydroxy-alpha-amino acids (2010), Appl. Microbiol. Biotechnol., 88, 409-424.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.5 0.19
-
 L-allo-threonine pH and temperature not specified in the publication Escherichia coli
4.1.2.5 10
-
 L-threonine pH and temperature not specified in the publication Escherichia coli
4.1.2.5 14.6
-
 L-allo-threonine pH and temperature not specified in the publication Pseudomonas sp.
4.1.2.5 14.7
-
 L-threonine pH and temperature not specified in the publication Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.5 Clostridium pasteurianum
-
-
-
4.1.2.5 Escherichia coli
-
-
-
4.1.2.5 Pseudomonas aeruginosa
-
-
-
4.1.2.5 Pseudomonas sp.
-
-
-
4.1.2.5 Pseudomonas sp. NCIMB10558
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.5 DEAE-cellulose column chromatography Clostridium pasteurianum
4.1.2.5 DEAE-Toyopearl column chromatography Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.5 additional information
-
 specific activity of 31 units/mg, using L-threonine as substrate, pH and temperature not specified in the publication Pseudomonas sp.
4.1.2.5 additional information
-
 the crude extract has a specific activity of 0.02 units/mg, the purified enzyme has a specific activity of 0.32 units/mg, pH and temperature not specified in the publication Clostridium pasteurianum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.5 L-allo-threonine
-
 Escherichia coli ?
-
 ?
4.1.2.5 L-allo-threonine
-
 Pseudomonas sp. ?
-
 ?
4.1.2.5 L-allo-threonine
-
 Pseudomonas sp. NCIMB10558 ?
-
 ?
4.1.2.5 L-threonine L-TA preferably accepts L-threonine as substrate Escherichia coli glycine + acetaldehyde
-
 ?
4.1.2.5 L-threonine L-TA preferably accepts L-threonine as substrate Pseudomonas aeruginosa glycine + acetaldehyde
-
 ?
4.1.2.5 L-threonine L-TA preferably accepts L-threonine as substrate Pseudomonas sp. glycine + acetaldehyde
-
 ?
4.1.2.5 L-threonine L-TA preferably accepts L-threonine as substrate Clostridium pasteurianum glycine + acetaldehyde
-
 ?
4.1.2.5 L-threonine L-TA preferably accepts L-threonine as substrate Pseudomonas sp. NCIMB10558 glycine + acetaldehyde
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.5 L-TA
-
 Escherichia coli
4.1.2.5 L-TA
-
 Pseudomonas aeruginosa
4.1.2.5 L-TA
-
 Pseudomonas sp.
4.1.2.5 L-TA
-
 Clostridium pasteurianum
4.1.2.5 L-threonine aldolase
-
 Escherichia coli
4.1.2.5 L-threonine aldolase
-
 Pseudomonas aeruginosa
4.1.2.5 L-threonine aldolase
-
 Pseudomonas sp.
4.1.2.5 L-threonine aldolase
-
 Clostridium pasteurianum

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.5 pyridoxal 5'-phosphate dependent on Escherichia coli
4.1.2.5 pyridoxal 5'-phosphate dependent on Pseudomonas aeruginosa
4.1.2.5 pyridoxal 5'-phosphate dependent on Pseudomonas sp.
4.1.2.5 pyridoxal 5'-phosphate dependent on Clostridium pasteurianum