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Literature summary extracted from

  • Wang, K.; Li, G.; Yu, S.Q.; Zhang, C.T.; Liu, Y.H.
    A novel metagenome-derived beta-galactosidase: gene cloning, overexpression, purification and characterization (2010), Appl. Microbiol. Biotechnol., 88, 155-165.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.23 expressed in Pichia pastoris as a His-tagged fusion protein uncultured soil bacterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 Cu2+ inhibitory effect uncultured soil bacterium
3.2.1.23 Zn2+ inhibitory effect uncultured soil bacterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 1.71
-
 2-nitrophenyl beta-D-galactopyranoside pH 7, 38°C uncultured soil bacterium
3.2.1.23 3.4
-
 2-nitrophenyl beta-D-galactopyranoside pH 7, 20°C uncultured soil bacterium
3.2.1.23 4.7
-
 2-nitrophenyl beta-D-galactopyranoside pH 7, 10°C uncultured soil bacterium
3.2.1.23 6.3
-
 2-nitrophenyl beta-D-galactopyranoside pH 7, 5°C uncultured soil bacterium
3.2.1.23 12.2
-
 lactose pH 7, 38°C uncultured soil bacterium
3.2.1.23 17.6
-
 lactose pH 7, 20°C uncultured soil bacterium
3.2.1.23 27.1
-
 lactose pH 7, 10°C uncultured soil bacterium
3.2.1.23 58.6
-
 lactose pH 7, 5°C uncultured soil bacterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.23 Ca2+ slightly stimulates activity uncultured soil bacterium
3.2.1.23 K+ slightly stimulates activity uncultured soil bacterium
3.2.1.23 Na+ slightly stimulates activity uncultured soil bacterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 78600
-
 calculated from cDNA uncultured soil bacterium
3.2.1.23 90000
-
 His-tagged fusion protein, SDS-PAGE uncultured soil bacterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.23 lactose + H2O uncultured soil bacterium
-
 D-galactose + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 uncultured soil bacterium
-
 a metagenomic library from topsoil of Daqing oil field of Heilongjiang Province in China is constructed for the screening of clones with beta-galactosidase activity
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.23 25.4
-
 38°C, pH 7, substrate: lactose uncultured soil bacterium
3.2.1.23 243
-
 38°C, pH 7, substrate: 2-nitrophenyl-beta-D-galactopyranoside uncultured soil bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 uncultured soil bacterium 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-arabinoside + H2O
-
 uncultured soil bacterium 4-nitrophenol + beta-D-arabinose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 uncultured soil bacterium 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-glucuronide + H2O
-
 uncultured soil bacterium 4-nitrophenol + beta-D-glucuronate
-
 ?
3.2.1.23 4-nitrophenyl beta-D-mannoside + H2O
-
 uncultured soil bacterium 4-nitrophenol + beta-D-mannose
-
 ?
3.2.1.23 lactose + H2O
-
 uncultured soil bacterium D-galactose + D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.23 beta-galactosidase
-
 uncultured soil bacterium
3.2.1.23 ZD410
-
 uncultured soil bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 38
-
-
 uncultured soil bacterium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.23
-
 20 11% activity at 0°C, 54% activity at 20°C uncultured soil bacterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 20 45 ZD410 is stable up to 30°C and has only 17% of its activity at 45°C. It is completely inactivated at 50°C uncultured soil bacterium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.23 2 3.7 2-nitrophenyl beta-D-galactopyranoside pH 7, 5°C uncultured soil bacterium
3.2.1.23 33
-
 lactose pH 7, 10°C uncultured soil bacterium
3.2.1.23 53
-
 lactose pH 7, 5°C uncultured soil bacterium
3.2.1.23 66.7
-
 2-nitrophenyl beta-D-galactopyranoside pH 7, 10°C uncultured soil bacterium
3.2.1.23 92
-
 lactose pH 7, 20°C uncultured soil bacterium
3.2.1.23 141
-
 lactose pH 7, 38°C uncultured soil bacterium
3.2.1.23 149
-
 2-nitrophenyl beta-D-galactopyranoside pH 7, 20°C uncultured soil bacterium
3.2.1.23 386
-
 2-nitrophenyl beta-D-galactopyranoside pH 7, 38°C uncultured soil bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 7
-
-
 uncultured soil bacterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.23 6 8 80% activity uncultured soil bacterium

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.23 0.9
-
 lactose pH 7, 5°C uncultured soil bacterium
3.2.1.23 1.21
-
 lactose pH 7, 10°C uncultured soil bacterium
3.2.1.23 3.7
-
 2-nitrophenyl beta-D-galactopyranoside pH 7, 5°C uncultured soil bacterium
3.2.1.23 5.23
-
 lactose pH 7, 20°C uncultured soil bacterium
3.2.1.23 11.6
-
 lactose pH 7, 38°C uncultured soil bacterium
3.2.1.23 14.2
-
 2-nitrophenyl beta-D-galactopyranoside pH 7, 10°C uncultured soil bacterium
3.2.1.23 43.7
-
 2-nitrophenyl beta-D-galactopyranoside pH 7, 20°C uncultured soil bacterium
3.2.1.23 226
-
 2-nitrophenyl beta-D-galactopyranoside pH 7, 38°C uncultured soil bacterium