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Literature summary extracted from

  • Zhou, J.; Shi, P.; Huang, H.; Cao, Y.; Meng, K.; Yang, P.; Zhang, R.; Chen, X.; Yao, B.
    A new beta-galactosidase from symbiotic Flavobacterium sp. TN17 reveals four residues essential for beta-galactosidase activity of gastrointestinal bacteria (2010), Appl. Microbiol. Biotechnol., 88, 1297-1309.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.22 beta-mercaptoethanol activity enhanced by 2fold Flavobacterium sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.22 expressed in Escherichia coli as a His-tagged fusion protein Flavobacterium sp.

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.22 D480A mutant shows no activity Flavobacterium sp.
3.2.1.22 D549A mutant shows no activity Flavobacterium sp.
3.2.1.22 D552A 81.2% activity compared to wild-type using 4-nitrophenyl-alpha-D-galactopyranoside as a substrate Flavobacterium sp.
3.2.1.22 R556A 27.6% activity compared to wild-type using 4-nitrophenyl-alpha-D-galactopyranoside as a substrate Flavobacterium sp.
3.2.1.22 S548A mutant shows no activity Flavobacterium sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.22 Ag+ completely inhibits activity Flavobacterium sp.
3.2.1.22 Cr3+ partial inhibition Flavobacterium sp.
3.2.1.22 Fe2+ partial inhibition Flavobacterium sp.
3.2.1.22 Hg2+ completely inhibits activity Flavobacterium sp.
3.2.1.22 SDS completely inhibits activity Flavobacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.22 2.47
-
4-nitrophenyl-alpha-D-galactopyranoside pH 5.5, 45°C Flavobacterium sp.
3.2.1.22 12.58
-
melibiose pH 5.5, 45°C Flavobacterium sp.
3.2.1.22 14.76
-
stachyose pH 5.5, 45°C Flavobacterium sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.22 EDTA activity enhanced by 2fold Flavobacterium sp.
3.2.1.22 Mn2+ activity enhanced by 2fold Flavobacterium sp.
3.2.1.22 Zn2+ activity enhanced by 2fold Flavobacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.22 82000
-
SDS-PAGE Flavobacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Flavobacterium sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.22 using Ni-NTA chromatography Flavobacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 4-nitrophenyl-alpha-D-galactopyranoside + H2O
-
Flavobacterium sp. 4-nitrophenol + alpha-D-galactopyranose
-
?
3.2.1.22 guar gum + H2O
-
Flavobacterium sp. ?
-
?
3.2.1.22 locust bean gum + H2O
-
Flavobacterium sp. ?
-
?
3.2.1.22 melibiose + H2O
-
Flavobacterium sp. alpha-D-galactose + alpha-D-glucose
-
?
3.2.1.22 raffinose + H2O
-
Flavobacterium sp. sucrose + alpha-D-galactose
-
?
3.2.1.22 stachyose + 2 H2O
-
Flavobacterium sp. 2 alpha-D-galactose + sucrose
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.22 alpha-galactosidase
-
Flavobacterium sp.
3.2.1.22 galA17
-
Flavobacterium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.22 45
-
assay at Flavobacterium sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.22 30 55 55% activity retained Flavobacterium sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.22 2.33
-
melibiose pH 5.5, 45°C Flavobacterium sp.
3.2.1.22 42.04
-
stachyose pH 5.5, 45°C Flavobacterium sp.
3.2.1.22 654.6
-
4-nitrophenyl-alpha-D-galactopyranoside pH 5.5, 45°C Flavobacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.22 5.5
-
assay at Flavobacterium sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.22 5 7 55% activity retained Flavobacterium sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.22 5 9 enzyme is stable over a wide pH range, retaining more than 80% of the initial activity after incubation in buffers ranging from pH 5.0 to 9.0 at 37°C for 1 h Flavobacterium sp.