EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.22 | beta-mercaptoethanol | activity enhanced by 2fold | Flavobacterium sp. |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.22 | expressed in Escherichia coli as a His-tagged fusion protein | Flavobacterium sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.22 | D480A | mutant shows no activity | Flavobacterium sp. |
3.2.1.22 | D549A | mutant shows no activity | Flavobacterium sp. |
3.2.1.22 | D552A | 81.2% activity compared to wild-type using 4-nitrophenyl-alpha-D-galactopyranoside as a substrate | Flavobacterium sp. |
3.2.1.22 | R556A | 27.6% activity compared to wild-type using 4-nitrophenyl-alpha-D-galactopyranoside as a substrate | Flavobacterium sp. |
3.2.1.22 | S548A | mutant shows no activity | Flavobacterium sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.22 | Ag+ | completely inhibits activity | Flavobacterium sp. | |
3.2.1.22 | Cr3+ | partial inhibition | Flavobacterium sp. | |
3.2.1.22 | Fe2+ | partial inhibition | Flavobacterium sp. | |
3.2.1.22 | Hg2+ | completely inhibits activity | Flavobacterium sp. | |
3.2.1.22 | SDS | completely inhibits activity | Flavobacterium sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.22 | 2.47 | - |
4-nitrophenyl-alpha-D-galactopyranoside | pH 5.5, 45°C | Flavobacterium sp. | |
3.2.1.22 | 12.58 | - |
melibiose | pH 5.5, 45°C | Flavobacterium sp. | |
3.2.1.22 | 14.76 | - |
stachyose | pH 5.5, 45°C | Flavobacterium sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.22 | EDTA | activity enhanced by 2fold | Flavobacterium sp. | |
3.2.1.22 | Mn2+ | activity enhanced by 2fold | Flavobacterium sp. | |
3.2.1.22 | Zn2+ | activity enhanced by 2fold | Flavobacterium sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 82000 | - |
SDS-PAGE | Flavobacterium sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.22 | Flavobacterium sp. | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.22 | using Ni-NTA chromatography | Flavobacterium sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.22 | 4-nitrophenyl-alpha-D-galactopyranoside + H2O | - |
Flavobacterium sp. | 4-nitrophenol + alpha-D-galactopyranose | - |
? | |
3.2.1.22 | guar gum + H2O | - |
Flavobacterium sp. | ? | - |
? | |
3.2.1.22 | locust bean gum + H2O | - |
Flavobacterium sp. | ? | - |
? | |
3.2.1.22 | melibiose + H2O | - |
Flavobacterium sp. | alpha-D-galactose + alpha-D-glucose | - |
? | |
3.2.1.22 | raffinose + H2O | - |
Flavobacterium sp. | sucrose + alpha-D-galactose | - |
? | |
3.2.1.22 | stachyose + 2 H2O | - |
Flavobacterium sp. | 2 alpha-D-galactose + sucrose | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.22 | alpha-galactosidase | - |
Flavobacterium sp. |
3.2.1.22 | galA17 | - |
Flavobacterium sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 45 | - |
assay at | Flavobacterium sp. |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 30 | 55 | 55% activity retained | Flavobacterium sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.22 | 2.33 | - |
melibiose | pH 5.5, 45°C | Flavobacterium sp. | |
3.2.1.22 | 42.04 | - |
stachyose | pH 5.5, 45°C | Flavobacterium sp. | |
3.2.1.22 | 654.6 | - |
4-nitrophenyl-alpha-D-galactopyranoside | pH 5.5, 45°C | Flavobacterium sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 5.5 | - |
assay at | Flavobacterium sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 5 | 7 | 55% activity retained | Flavobacterium sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 5 | 9 | enzyme is stable over a wide pH range, retaining more than 80% of the initial activity after incubation in buffers ranging from pH 5.0 to 9.0 at 37°C for 1 h | Flavobacterium sp. |