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Literature summary extracted from

  • Bai, Y.; Wang, J.; Zhang, Z.; Shi, P.; Luo, H.; Huang, H.; Luo, C.; Yao, B.
    A novel family 9 beta-1,3(4)-glucanase from thermoacidophilic Alicyclobacillus sp. A4 with potential applications in the brewing industry (2010), Appl. Microbiol. Biotechnol., 87, 251-259.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.6 2-mercaptoethanol 2 mM, 119% of initial activity Alicyclobacillus sp.

Application

EC Number Application Comment Organism
3.2.1.6 food industry application in malting and brewing industry. Under simulated mashing conditions, addition of Agl9A at 20 U/ml or a commercial xylanase at 200 U/ml reduce the filtration rate by 26.71% and 20.21%, respectively, and viscosity by 6.12% and 4.78%, respectively. Combined use of Agl9A at 10 U/ml and the xylanase at 100 U/ml even more effectively reduces the filtration rate by 31.73% and viscosity by 8.79% Alicyclobacillus sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.6 expression in Pichia pastoris Alicyclobacillus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.6 Ag+ 2 mM, complete loss of activity Alicyclobacillus sp.
3.2.1.6 Cr3+ 2 mM, 78% of initial activity Alicyclobacillus sp.
3.2.1.6 Cu2+ 2 mM, 23% of initial activity Alicyclobacillus sp.
3.2.1.6 EDTA 2 mM, 64% of initial activity Alicyclobacillus sp.
3.2.1.6 Hg2+ 2 mM, complete loss of activity Alicyclobacillus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.6 Co2+ 2 mM, 113% of initial activity Alicyclobacillus sp.
3.2.1.6 Mn2+ 2 mM, 123% of initial activity Alicyclobacillus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.6 60000
-
 x * 60000, SDS-PAGE, x * 60700, calculated Alicyclobacillus sp.
3.2.1.6 60700
-
 x * 60000, SDS-PAGE, x * 60700, calculated Alicyclobacillus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.6 Alicyclobacillus sp. D7PC10
-
-

Storage Stability

EC Number Storage Stability Organism
3.2.1.6 4°C, 0.1 M McIlvaine buffer, pH 5.8, retains almost all initial activity after 1 week of storage Alicyclobacillus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.6 beta-D-glucan + H2O source of substrate: barley Alicyclobacillus sp. ?
-
 ?
3.2.1.6 carboxymethyl-cellulose + H2O 15% of the activity with beta-D-glucan Alicyclobacillus sp. ?
-
 ?
3.2.1.6 laminarin + H2O 6.2% of the activity with beta-D-glucan Alicyclobacillus sp. ?
-
 ?
3.2.1.6 lichenan + H2O 109% of the activity with beta-D-glucan Alicyclobacillus sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.6 ? x * 60000, SDS-PAGE, x * 60700, calculated Alicyclobacillus sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.6 Agl9A
-
 Alicyclobacillus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.6 55
-
-
 Alicyclobacillus sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.6 60
-
 rapid loss of activity above Alicyclobacillus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.6 60
-
 1 h, more than 70% residual activity Alicyclobacillus sp.
3.2.1.6 70
-
 30 min, 20% residual activity Alicyclobacillus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.6 5.8
-
-
 Alicyclobacillus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.6 5 8 more than 65% of maximum activity within Alicyclobacillus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.6 3.8 11.2 1 h, more than 60% residual activity Alicyclobacillus sp.

General Information

EC Number General Information Comment Organism
3.2.1.6 metabolism enzyme is highly resistant to trypsin, alpha-chymotrypsin, collagenase and Neutrase 0.8L Alicyclobacillus sp.