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Literature summary extracted from

  • Pollet, A.; Schoepe, J.; Dornez, E.; Strelkov, S.; Delcour, J.; Courtin, C.
    Functional analysis of glycoside hydrolase family 8 xylanases shows narrow but distinct substrate specificities and biotechnological potential (2010), Appl. Microbiol. Biotechnol., 87, 2125-2135.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.156 expressed in Escherichia coli BL21(DE3)pLysS cells Bifidobacterium adolescentis
3.2.1.156 expressed in Escherichia coli BL21(DE3)pLysS cells uncultured bacterium
3.2.1.156 expressed in Escherichia coli BL21(DE3)pLysS cells Halalkalibacterium halodurans
3.2.1.156 expressed in Escherichia coli BL21(DE3)pLysS cells Pseudoalteromonas haloplanktis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.156 Bifidobacterium adolescentis
-
-
-
3.2.1.156 Halalkalibacterium halodurans
-
-
-
3.2.1.156 Pseudoalteromonas haloplanktis
-
-
-
3.2.1.156 uncultured bacterium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.156 Ni-NTA affinity column chromatography, HisTrap HP column chromatography, Q Sepharose column chromatography, and SP Sepharose column chromatography Bifidobacterium adolescentis
3.2.1.156 Ni-NTA affinity column chromatography, HisTrap HP column chromatography, Q Sepharose column chromatography, and SP Sepharose column chromatography uncultured bacterium
3.2.1.156 Ni-NTA affinity column chromatography, HisTrap HP column chromatography, Q Sepharose column chromatography, and SP Sepharose column chromatography Halalkalibacterium halodurans
3.2.1.156 Ni-NTA affinity column chromatography, HisTrap HP column chromatography, Q Sepharose column chromatography, and SP Sepharose column chromatography Pseudoalteromonas haloplanktis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.156 2-nitrophenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta + H2O
-
 Bifidobacterium adolescentis 2-nitrophenyl beta-D-xylopyranose + Xylbeta(1-4)Xylbeta(1-4)Xylbeta
-
 ?
3.2.1.156 2-nitrophenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta + H2O
-
 Halalkalibacterium halodurans 2-nitrophenyl beta-D-xylopyranose + Xylbeta(1-4)Xylbeta(1-4)Xylbeta
-
 ?
3.2.1.156 2-nitrophenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta + H2O
-
 Pseudoalteromonas haloplanktis 2-nitrophenyl beta-D-xylopyranose + Xylbeta(1-4)Xylbeta(1-4)Xylbeta
-
 ?
3.2.1.156 2-nitrophenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta + H2O the enzyme has a strong preference for hydrolysis of the third xylosidic bond from the non-reducing end of the substrate uncultured bacterium 2-nitrophenyl beta-D-xylopyranose + Xylbeta(1-4)Xylbeta(1-4)Xylbeta
-
 ?
3.2.1.156 2-nitrophenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta + H2O
-
 Bifidobacterium adolescentis 2-nitrophenyl Xylbeta(1-4)Xylbeta + Xylbeta(1-4)Xylbeta(1-4)Xyl
-
 ?
3.2.1.156 2-nitrophenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta + H2O
-
 uncultured bacterium 2-nitrophenyl Xylbeta(1-4)Xylbeta + Xylbeta(1-4)Xylbeta(1-4)Xyl
-
 ?
3.2.1.156 2-nitrophenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta + H2O
-
 Halalkalibacterium halodurans 2-nitrophenyl Xylbeta(1-4)Xylbeta + Xylbeta(1-4)Xylbeta(1-4)Xyl
-
 ?
3.2.1.156 2-nitrophenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta + H2O
-
 Pseudoalteromonas haloplanktis 2-nitrophenyl Xylbeta(1-4)Xylbeta + Xylbeta(1-4)Xylbeta(1-4)Xyl
-
 ?
3.2.1.156 2-nitrophenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta + H2O the enzyme cleaves the substrate exclusively at the third xylosidic bond from the reducing end uncultured bacterium 2-nitrophenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta + Xylbeta(1-4)Xylbeta(1-4)Xyl
-
 ?
3.2.1.156 2-nitrophenyl xylohexaose + H2O
-
 Bifidobacterium adolescentis ?
-
 ?
3.2.1.156 2-nitrophenyl xylohexaose + H2O
-
 Halalkalibacterium halodurans ?
-
 ?
3.2.1.156 2-nitrophenyl xylohexaose + H2O the enzyme hydrolyzes 2-nitrophenyl xylohexaose mainly at the second and third xylosidic bonds from the reducing end of the substrate Pseudoalteromonas haloplanktis ?
-
 ?
3.2.1.156 additional information no activity towards xyloheptaose, xylooctaose, and xylononaose Halalkalibacterium halodurans ?
-
 ?
3.2.1.156 additional information no activity towards xylotriose and xylotetraose uncultured bacterium ?
-
 ?
3.2.1.156 additional information no activity with xylotriose, xylotetraose, and xylopentaose Pseudoalteromonas haloplanktis ?
-
 ?
3.2.1.156 oat spelt xylan + H2O
-
 uncultured bacterium ?
-
 ?
3.2.1.156 oat spelt xylan + H2O
-
 Pseudoalteromonas haloplanktis ?
-
 ?
3.2.1.156 soluble oat spelt xylan + H2O
-
 uncultured bacterium ?
-
 ?
3.2.1.156 soluble oat spelt xylan + H2O
-
 Pseudoalteromonas haloplanktis ?
-
 ?
3.2.1.156 wheat flour arabinoxylan + H2O
-
 uncultured bacterium ?
-
 ?
3.2.1.156 wheat flour arabinoxylan + H2O
-
 Pseudoalteromonas haloplanktis ?
-
 ?
3.2.1.156 xyloheptaose + H2O
-
 uncultured bacterium ?
-
 ?
3.2.1.156 xyloheptaose + H2O
-
 Pseudoalteromonas haloplanktis ?
-
 ?
3.2.1.156 xylohexaose + H2O
-
 uncultured bacterium ?
-
 ?
3.2.1.156 xylohexaose + H2O
-
 Halalkalibacterium halodurans ?
-
 ?
3.2.1.156 xylohexaose + H2O
-
 Pseudoalteromonas haloplanktis ?
-
 ?
3.2.1.156 xylononaose + H2O
-
 uncultured bacterium ?
-
 ?
3.2.1.156 xylononaose + H2O
-
 Pseudoalteromonas haloplanktis ?
-
 ?
3.2.1.156 xylooctaose + H2O
-
 uncultured bacterium ?
-
 ?
3.2.1.156 xylooctaose + H2O
-
 Pseudoalteromonas haloplanktis ?
-
 ?
3.2.1.156 xylopentaose + H2O
-
 uncultured bacterium ?
-
 ?
3.2.1.156 xylopentaose + H2O
-
 Halalkalibacterium halodurans ?
-
 ?
3.2.1.156 xylotetraose + H2O
-
 Halalkalibacterium halodurans ?
-
 ?
3.2.1.156 xylotriose + H2O
-
 Halalkalibacterium halodurans ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.156 PhXyl
-
 Pseudoalteromonas haloplanktis
3.2.1.156 reducing-end xylose-releasing exooligoxylanase
-
 Bifidobacterium adolescentis
3.2.1.156 reducing-end xylose-releasing exooligoxylanase
-
 uncultured bacterium
3.2.1.156 reducing-end xylose-releasing exooligoxylanase
-
 Halalkalibacterium halodurans
3.2.1.156 reducing-end xylose-releasing exooligoxylanase
-
 Pseudoalteromonas haloplanktis
3.2.1.156 Rex
-
 Bifidobacterium adolescentis
3.2.1.156 RexA
-
 Halalkalibacterium halodurans
3.2.1.156 rXyn8
-
 uncultured bacterium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.156 138
-
 oat spelt xylan apparent value, at pH 6.0, 40°C uncultured bacterium
3.2.1.156 166
-
 oat spelt xylan apparent value, at pH 6.0, 40°C Pseudoalteromonas haloplanktis
3.2.1.156 1099
-
 wheat flour arabinoxylan apparent value, at pH 6.0, 40°C uncultured bacterium
3.2.1.156 1455
-
 wheat flour arabinoxylan apparent value, at pH 6.0, 40°C Pseudoalteromonas haloplanktis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.156 5
-
 xylohexaose apparent value, at pH 6.0, 40°C Pseudoalteromonas haloplanktis
3.2.1.156 22
-
 xylopentaose apparent value, at pH 6.0, 40°C uncultured bacterium
3.2.1.156 25
-
 xylohexaose apparent value, at pH 6.0, 40°C Halalkalibacterium halodurans
3.2.1.156 30
-
 2-nitrophenyl Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta apparent value, at pH 6.0, 40°C Halalkalibacterium halodurans
3.2.1.156 32
-
 xyloheptaose apparent value, at pH 6.0, 40°C Pseudoalteromonas haloplanktis
3.2.1.156 43
-
 xylotetraose apparent value, at pH 6.0, 40°C Halalkalibacterium halodurans
3.2.1.156 65
-
 xylotriose apparent value, at pH 6.0, 40°C Halalkalibacterium halodurans
3.2.1.156 67
-
 xylooctaose apparent value, at pH 6.0, 40°C Pseudoalteromonas haloplanktis
3.2.1.156 92
-
 xylononaose apparent value, at pH 6.0, 40°C Pseudoalteromonas haloplanktis
3.2.1.156 105
-
 xyloheptaose apparent value, at pH 6.0, 40°C uncultured bacterium
3.2.1.156 132
-
 xylooctaose apparent value, at pH 6.0, 40°C uncultured bacterium
3.2.1.156 152
-
 xylononaose apparent value, at pH 6.0, 40°C uncultured bacterium
3.2.1.156 158
-
 xylohexaose apparent value, at pH 6.0, 40°C uncultured bacterium