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Literature summary extracted from

  • Nguyen, N.P.; Lee, K.M.; Lee, K.M.; Kim, I.W.; Kim, Y.S.; Jeya, M.; Lee, J.K.
    One-step purification and characterization of a beta-1,4-glucosidase from a newly isolated strain of Stereum hirsutum (2010), Appl. Microbiol. Biotechnol., 87, 2107-2116.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.74 ethanol 5% (v/v) ethanol increases the enzyme activity about 30% Stereum hirsutum
3.2.1.74 methanol 5% (v/v) methanol increases the enzyme activity about 30% Stereum hirsutum
3.2.1.74 additional information thiol groups are not essential for catalytic activity, the enzyme is not affected by Tween 20 Stereum hirsutum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.74 Cu2+ competitive inhibition, 26.7% residual activity at 10 mM Stereum hirsutum
3.2.1.74 D-glucose D-glucose inhibition is competitive for 4-nitrophenyl beta-D-glucopyranoside Stereum hirsutum
3.2.1.74 Fe3+ 80.5% residual activity at 10 mM Stereum hirsutum
3.2.1.74 Hg2+ competitive inhibition, 4.3% residual activity at 10 mM Stereum hirsutum
3.2.1.74 additional information not inhibited by EDTA, N-ethylmaleimide, beta-mercaptoethanol or cysteine. Ca2+, Co2+, Mg2+, Mn2+, and Zn2+ have no inhibitory effect Stereum hirsutum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.74 2.5
-
4-nitrophenyl beta-D-glucopyranoside in 100 mM sodium acetate buffer (pH 4.5), at 65°C Stereum hirsutum
3.2.1.74 86
-
cellobiose in 100 mM sodium acetate buffer (pH 4.5), at 65°C Stereum hirsutum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.74 additional information divalent cations are not required for enzyme activation Stereum hirsutum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.74 98000
-
8 * 98000, SDS-PAGE Stereum hirsutum
3.2.1.74 780000
-
gel filtration Stereum hirsutum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.74 Stereum hirsutum
-
-
-
3.2.1.74 Stereum hirsutum SKU512
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.74 glycoprotein
-
Stereum hirsutum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.74 Superdex 200 gel filtration Stereum hirsutum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.74 2-nitrophenyl beta-cellobioside + H2O 3.1% activity compared to 4-nitrophenyl beta-D-glucopyranoside Stereum hirsutum 2-nitrophenol + beta-cellobiose
-
?
3.2.1.74 2-nitrophenyl beta-D-glucopyranoside + H2O 25.2% activity compared to 4-nitrophenyl beta-D-glucopyranoside Stereum hirsutum 2-nitrophenol + D-glucopyranose
-
?
3.2.1.74 2-nitrophenyl beta-D-glucopyranoside + H2O 25.2% activity compared to 4-nitrophenyl beta-D-glucopyranoside Stereum hirsutum SKU512 2-nitrophenol + D-glucopyranose
-
?
3.2.1.74 4-nitrophenyl beta-cellobioside + H2O 6.5% activity compared to 4-nitrophenyl beta-D-glucopyranoside Stereum hirsutum 4-nitrophenol + beta-cellobiose
-
?
3.2.1.74 4-nitrophenyl beta-D-galactopyranoside + H2O 1.2% activity compared to 4-nitrophenyl beta-D-glucopyranoside Stereum hirsutum 4-nitrophenol + beta-D-galactopyranose
-
?
3.2.1.74 4-nitrophenyl beta-D-glucopyranoside + H2O 100% activity Stereum hirsutum 4-nitrophenol + D-glucopyranose
-
?
3.2.1.74 4-nitrophenyl beta-D-glucopyranoside + H2O 100% activity Stereum hirsutum SKU512 4-nitrophenol + D-glucopyranose
-
?
3.2.1.74 cellobiose + H2O 0.96% activity compared to 4-nitrophenyl beta-D-glucopyranoside Stereum hirsutum 2 beta-D-glucose
-
?
3.2.1.74 cellobiose + H2O 0.96% activity compared to 4-nitrophenyl beta-D-glucopyranoside Stereum hirsutum SKU512 2 beta-D-glucose
-
?
3.2.1.74 additional information no activity with cellotriose, cellotetraose, cellopentaose, carboxy-methylcellulose, xylan, laminarin, avicel (2% w/v), 4-nitrophenyl beta-D-mannopyranoside, and 4-nitrophenyl alpha-D-glucopyranoside Stereum hirsutum ?
-
?
3.2.1.74 additional information the enzyme is also able to promote transglycosylation using methanol as an acceptor and glucose as a donor Stereum hirsutum ?
-
?
3.2.1.74 additional information no activity with cellotriose, cellotetraose, cellopentaose, carboxy-methylcellulose, xylan, laminarin, avicel (2% w/v), 4-nitrophenyl beta-D-mannopyranoside, and 4-nitrophenyl alpha-D-glucopyranoside Stereum hirsutum SKU512 ?
-
?
3.2.1.74 additional information the enzyme is also able to promote transglycosylation using methanol as an acceptor and glucose as a donor Stereum hirsutum SKU512 ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.74 homooctamer 8 * 98000, SDS-PAGE Stereum hirsutum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.74 beta-1,4-glucosidase
-
Stereum hirsutum
3.2.1.74 Bgl
-
Stereum hirsutum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.74 65
-
-
Stereum hirsutum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.74 60 70 85% and 91% of the maximum activity at 60°C and 70°C, respectively Stereum hirsutum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.74 50 70 at 70°C, 65°C, 60°C, and 50°C the enzyme retains 50% of the initial activity after 2, 10, 16, and 35 min, respectively Stereum hirsutum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.74 281
-
cellobiose in 100 mM sodium acetate buffer (pH 4.5), at 65°C Stereum hirsutum
3.2.1.74 4945
-
4-nitrophenyl beta-D-glucopyranoside in 100 mM sodium acetate buffer (pH 4.5), at 65°C Stereum hirsutum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.74 4.5
-
-
Stereum hirsutum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.74 4 5 84% and 86% of the maximum activity appears at pH 4.0 and 5.0, respectively Stereum hirsutum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.74 2 5.5 after 24 h of incubation at 20°C, the enzyme is fairly stable at the acidic pH ranging from 3.0 to 5.5. About 15% and 76% of activity remain after incubation at pH 2.0 and 2.5, respectively Stereum hirsutum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.74 29
-
D-glucose in 100 mM sodium acetate buffer (pH 4.5), at 65°C Stereum hirsutum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.74 Stereum hirsutum isoelectric focusing
-
4.9