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Literature summary extracted from

  • Wong, D.; Chan, V.; McCormack, A.; Batt, S.
    A novel xyloglucan-specific endo-beta-1,4-glucanase: Biochemical properties and inhibition studies (2010), Appl. Microbiol. Biotechnol., 86, 1463-1471.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.151 expressed in Escherichia coli BL21 (DE3)pLysE cells uncultured bacterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.151 XXXG the addition of XXXG results in linear noncompetitive inhibition on the hydrolysis of tamarind xyloglucan uncultured bacterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.151 45455
-
 x * 45455, calculated from amino acid sequence uncultured bacterium

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.151 uncultured bacterium D2K7Z0 from cow's rumen
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.151 carboxymethyl cellulose + H2O
-
 uncultured bacterium ?
-
 ?
3.2.1.151 lichenan + H2O
-
 uncultured bacterium ?
-
 ?
3.2.1.151 additional information the enzyme is devoid of transglycosylase activities. There is undetectable activity on cellulose, xylan, arbinoxylan, laminarin, or pectin uncultured bacterium ?
-
 ?
3.2.1.151 tamarind xyloglucan + H2O high specific activity uncultured bacterium ?
-
 ?
3.2.1.151 xyloglucan + H2O the enzyme catalyzes endohydrolysis of 1,4-beta-D-glucosidic linkages in xyloglucan uncultured bacterium xyloglucan oligosaccharides
-
 ?
3.2.1.151 xyloglucan oligosaccharide HDP-XGO + H2O
-
 uncultured bacterium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.151 ? x * 45455, calculated from amino acid sequence uncultured bacterium

Synonyms

EC Number Synonyms Comment Organism
3.2.1.151 endo-xyloglucanase
-
 uncultured bacterium
3.2.1.151 XEG5A
-
 uncultured bacterium
3.2.1.151 xyloglucan-specific endo-beta-1,4-glucanase
-
 uncultured bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.151 40 50
-
 uncultured bacterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.151 50 70 the enzyme is stable up to 50°C. The enzyme is completely inactivated by incubation at 70°C for 10 min uncultured bacterium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.151 1.24
-
 tamarind xyloglucan at 37°C, pH 7.0 uncultured bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.151 7
-
-
 uncultured bacterium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.151 5
-
 the enzyme is relatively stable at pH above 5.0, where the enzyme retains more than 95% of the activity uncultured bacterium

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.151 1.46
-
 XXXG pH and temperature not specified in the publication uncultured bacterium

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.151 uncultured bacterium calculated from amino acid sequence
-
 4.5