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Literature summary extracted from

  • Pai, C.; Wu, Z.; Chen, M.; Zeng, Y.; Chen, J.; Duan, C.; Li, M.; Liu, J.
    Molecular cloning and characterization of a bifunctional xylanolytic enzyme from Neocallimastix patriciarum (2010), Appl. Microbiol. Biotechnol., 85, 1451-1462.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.72 cloning of a bifunctional acetylxylan esterase/xylanase, XynS20E, DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression in Escherichia coli strain BL21 Neocallimastix patriciarum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.72 16.72
-
 birchwood xylan pH 8.2, 58°C Neocallimastix patriciarum

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.72 Neocallimastix patriciarum B8YG19 gene xynS20E
-
3.1.1.72 Neocallimastix patriciarum S20 B8YG19 gene xynS20E
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.72 recombinant enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and gel filtration Neocallimastix patriciarum

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.72 273.7
-
 acetylxylan esterase activity on birchwood xylan, pH 8.2, 58°C, purified recombinant enzyme Neocallimastix patriciarum
3.1.1.72 557.9
-
 acetylxylan esterase activity on oat spelt xylan, pH 8.2, 58°C, purified recombinant enzyme Neocallimastix patriciarum
3.1.1.72 580.3
-
 acetylxylan esterase activity on 4-methylumbelliferyl acetate, pH 8.2, 58°C, purified recombinant enzyme Neocallimastix patriciarum
3.1.1.72 873.1
-
 acetylxylan esterase activity on beechwood xylan, pH 8.2, 58°C, purified recombinant enzyme Neocallimastix patriciarum
3.1.1.72 1228
-
 acetylxylan esterase activity on beta-D-xylose tetraacetate, pH 8.2, 58°C, purified recombinant enzyme Neocallimastix patriciarum
3.1.1.72 2614
-
 acetylxylan esterase activity on acetylxylan, pH 8.2, 58°C, purified recombinant enzyme Neocallimastix patriciarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.72 4-methylumbelliferyl acetate + H2O
-
 Neocallimastix patriciarum 4-methylumbelliferol + acetate
-
 ?
3.1.1.72 4-methylumbelliferyl acetate + H2O
-
 Neocallimastix patriciarum S20 4-methylumbelliferol + acetate
-
 ?
3.1.1.72 acetylxylan + H2O
-
 Neocallimastix patriciarum ?
-
 ?
3.1.1.72 acetylxylan + H2O
-
 Neocallimastix patriciarum S20 ?
-
 ?
3.1.1.72 beechwood xylan + H2O
-
 Neocallimastix patriciarum ?
-
 ?
3.1.1.72 beechwood xylan + H2O
-
 Neocallimastix patriciarum S20 ?
-
 ?
3.1.1.72 beta-D-xylose tetraacetate + H2O
-
 Neocallimastix patriciarum ?
-
 ?
3.1.1.72 birchwood xylan + H2O
-
 Neocallimastix patriciarum ?
-
 ?
3.1.1.72 additional information the bifunctional acetylxylan esterase/xylanase shows xylanase and acetylxylan esterase activities on birchwood xylan Neocallimastix patriciarum ?
-
 ?
3.1.1.72 additional information the bifunctional acetylxylan esterase/xylanase shows xylanase and acetylxylan esterase activities on birchwood xylan Neocallimastix patriciarum S20 ?
-
 ?
3.1.1.72 oat spelt xylan + H2O
-
 Neocallimastix patriciarum ?
-
 ?
3.1.1.72 oat spelt xylan + H2O
-
 Neocallimastix patriciarum S20 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.72 More XynS20E contains a putative conserved domain of carbohydrate esterase family 1 at the N-terminus and a putative conserved domain of glycosyl hydrolase family 11 at the C-terminus Neocallimastix patriciarum

Synonyms

EC Number Synonyms Comment Organism
3.1.1.72 bifunctional acetylxylan esterase/xylanase
-
 Neocallimastix patriciarum
3.1.1.72 XynS20E
-
 Neocallimastix patriciarum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.72 58
-
 carbohydrate esterase activity Neocallimastix patriciarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.72 8.2
-
 carbohydrate esterase activity Neocallimastix patriciarum