BRENDA - Enzyme Database

Characterization of bacterial beta-carotene 3,3-hydroxylases, CrtZ, and P450 in astaxanthin biosynthetic pathway and adonirubin production by gene combination in Escherichia coli

Choi, S.; Matsuda, S.; Hoshino, T.; Peng, X.; Misawa, N.; Appl. Microbiol. Biotechnol. 72, 1238-1246 (2006)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.15.24
expressed in Escherichia coli JM109 cells
Brevundimonas sp.
1.14.15.24
expressed in Escherichia coli JM109 cells
marine bacterium P99-3
1.14.15.24
expressed in Escherichia coli JM109 cells
Pantoea ananatis
1.14.15.24
expressed in Escherichia coli JM109 cells; expressed in Escherichia coli JM109 cells
Paracoccus sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.15.24
Brevundimonas sp.
Q4W8B0
-
-
1.14.15.24
marine bacterium P99-3
Q7WT73
-
-
1.14.15.24
Pantoea ananatis
P21688
formerly known as Erwinia uredovora 20D3
-
1.14.15.24
Paracoccus sp.
P54973
formerly known as Agrobacterium aurantiacum
-
1.14.15.24
Paracoccus sp.
Q44262
formerly known as Alcaligenes sp. PC-1
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.24
3'-hydroxyechinenone + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
713863
marine bacterium P99-3
adonixanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
3'-hydroxyechinenone + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
713863
Brevundimonas sp.
adonixanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
3'-hydroxyechinenone + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
713863
Paracoccus sp.
adonixanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
3'-hydroxyechinenone + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
713863
Pantoea ananatis
adonixanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
3-hydroxyechinenone + NADH + H+ + O2
-
713863
marine bacterium P99-3
adonixanthin + NAD+ + H2O
-
-
-
?
1.14.15.24
3-hydroxyechinenone + NADH + H+ + O2
-
713863
Brevundimonas sp.
adonixanthin + NAD+ + H2O
-
-
-
?
1.14.15.24
3-hydroxyechinenone + NADH + H+ + O2
-
713863
Paracoccus sp.
adonixanthin + NAD+ + H2O
-
-
-
?
1.14.15.24
3-hydroxyechinenone + NADH + H+ + O2
-
713863
Pantoea ananatis
adonixanthin + NAD+ + H2O
-
-
-
?
1.14.15.24
adonirubin + NADH + H+ + O2
adonirubin is also named phoenicoxanthin or (3S)-3-hydroxy-beta,beta-carotene-4,4'-dione
713863
marine bacterium P99-3
astaxanthin + NAD+ + H2O
-
-
-
?
1.14.15.24
adonirubin + NADH + H+ + O2
adonirubin is also named phoenicoxanthin or (3S)-3-hydroxy-beta,beta-carotene-4,4'-dione
713863
Brevundimonas sp.
astaxanthin + NAD+ + H2O
-
-
-
?
1.14.15.24
adonirubin + NADH + H+ + O2
adonirubin is also named phoenicoxanthin or (3S)-3-hydroxy-beta,beta-carotene-4,4'-dione
713863
Paracoccus sp.
astaxanthin + NAD+ + H2O
-
-
-
?
1.14.15.24
adonirubin + NADH + H+ + O2
adonirubin is also named phoenicoxanthin or (3S)-3-hydroxy-beta,beta-carotene-4,4'-dione
713863
Pantoea ananatis
astaxanthin + NAD+ + H2O
-
-
-
?
1.14.15.24
beta-carotene + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
713863
marine bacterium P99-3
beta-cryptoxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
beta-carotene + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
713863
Brevundimonas sp.
beta-cryptoxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
beta-carotene + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
713863
Paracoccus sp.
beta-cryptoxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
beta-carotene + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
713863
Pantoea ananatis
beta-cryptoxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2
overall reaction
713863
marine bacterium P99-3
zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
1.14.15.24
beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2
overall reaction
713863
Brevundimonas sp.
zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
1.14.15.24
beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2
overall reaction
713863
Paracoccus sp.
zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
1.14.15.24
beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2
overall reaction
713863
Pantoea ananatis
zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
1.14.15.24
beta-cryptoxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
713863
marine bacterium P99-3
zeaxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
beta-cryptoxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
713863
Brevundimonas sp.
zeaxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
beta-cryptoxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
713863
Paracoccus sp.
zeaxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
beta-cryptoxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
713863
Pantoea ananatis
zeaxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
canthaxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
713863
marine bacterium P99-3
adonirubin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
canthaxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
713863
Brevundimonas sp.
adonirubin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
canthaxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
713863
Paracoccus sp.
adonirubin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
canthaxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
713863
Pantoea ananatis
adonirubin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
echinenone + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2
-
713863
marine bacterium P99-3
3'-hydroxyechinenone + 3-hydroxyechinenone + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
1.14.15.24
echinenone + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2
-
713863
Brevundimonas sp.
3'-hydroxyechinenone + 3-hydroxyechinenone + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
1.14.15.24
echinenone + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2
-
713863
Paracoccus sp.
3'-hydroxyechinenone + 3-hydroxyechinenone + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
1.14.15.24
echinenone + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2
-
713863
Pantoea ananatis
3'-hydroxyechinenone + 3-hydroxyechinenone + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.15.24
expressed in Escherichia coli JM109 cells
Brevundimonas sp.
1.14.15.24
expressed in Escherichia coli JM109 cells
marine bacterium P99-3
1.14.15.24
expressed in Escherichia coli JM109 cells
Pantoea ananatis
1.14.15.24
expressed in Escherichia coli JM109 cells
Paracoccus sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.24
3'-hydroxyechinenone + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
713863
marine bacterium P99-3
adonixanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
3'-hydroxyechinenone + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
713863
Brevundimonas sp.
adonixanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
3'-hydroxyechinenone + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
713863
Paracoccus sp.
adonixanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
3'-hydroxyechinenone + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
713863
Pantoea ananatis
adonixanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
3-hydroxyechinenone + NADH + H+ + O2
-
713863
marine bacterium P99-3
adonixanthin + NAD+ + H2O
-
-
-
?
1.14.15.24
3-hydroxyechinenone + NADH + H+ + O2
-
713863
Brevundimonas sp.
adonixanthin + NAD+ + H2O
-
-
-
?
1.14.15.24
3-hydroxyechinenone + NADH + H+ + O2
-
713863
Paracoccus sp.
adonixanthin + NAD+ + H2O
-
-
-
?
1.14.15.24
3-hydroxyechinenone + NADH + H+ + O2
-
713863
Pantoea ananatis
adonixanthin + NAD+ + H2O
-
-
-
?
1.14.15.24
adonirubin + NADH + H+ + O2
adonirubin is also named phoenicoxanthin or (3S)-3-hydroxy-beta,beta-carotene-4,4'-dione
713863
marine bacterium P99-3
astaxanthin + NAD+ + H2O
-
-
-
?
1.14.15.24
adonirubin + NADH + H+ + O2
adonirubin is also named phoenicoxanthin or (3S)-3-hydroxy-beta,beta-carotene-4,4'-dione
713863
Brevundimonas sp.
astaxanthin + NAD+ + H2O
-
-
-
?
1.14.15.24
adonirubin + NADH + H+ + O2
adonirubin is also named phoenicoxanthin or (3S)-3-hydroxy-beta,beta-carotene-4,4'-dione
713863
Paracoccus sp.
astaxanthin + NAD+ + H2O
-
-
-
?
1.14.15.24
adonirubin + NADH + H+ + O2
adonirubin is also named phoenicoxanthin or (3S)-3-hydroxy-beta,beta-carotene-4,4'-dione
713863
Pantoea ananatis
astaxanthin + NAD+ + H2O
-
-
-
?
1.14.15.24
beta-carotene + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
713863
marine bacterium P99-3
beta-cryptoxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
beta-carotene + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
713863
Brevundimonas sp.
beta-cryptoxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
beta-carotene + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
713863
Paracoccus sp.
beta-cryptoxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
beta-carotene + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
713863
Pantoea ananatis
beta-cryptoxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2
overall reaction
713863
marine bacterium P99-3
zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
1.14.15.24
beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2
overall reaction
713863
Brevundimonas sp.
zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
1.14.15.24
beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2
overall reaction
713863
Paracoccus sp.
zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
1.14.15.24
beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2
overall reaction
713863
Pantoea ananatis
zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
1.14.15.24
beta-cryptoxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
713863
marine bacterium P99-3
zeaxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
beta-cryptoxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
713863
Brevundimonas sp.
zeaxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
beta-cryptoxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
713863
Paracoccus sp.
zeaxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
beta-cryptoxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
713863
Pantoea ananatis
zeaxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
canthaxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
713863
marine bacterium P99-3
adonirubin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
canthaxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
713863
Brevundimonas sp.
adonirubin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
canthaxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
713863
Paracoccus sp.
adonirubin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
canthaxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
713863
Pantoea ananatis
adonirubin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.24
echinenone + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2
-
713863
marine bacterium P99-3
3'-hydroxyechinenone + 3-hydroxyechinenone + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
1.14.15.24
echinenone + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2
-
713863
Brevundimonas sp.
3'-hydroxyechinenone + 3-hydroxyechinenone + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
1.14.15.24
echinenone + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2
-
713863
Paracoccus sp.
3'-hydroxyechinenone + 3-hydroxyechinenone + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
1.14.15.24
echinenone + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2
-
713863
Pantoea ananatis
3'-hydroxyechinenone + 3-hydroxyechinenone + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
1.14.15.24
metabolism
CrtZ is one of ratelimiting enzymes for astaxanthin ((3S,3'S)-3,3'-dihydroxy-beta,beta-carotene-4,4'-dione) production
Brevundimonas sp.
1.14.15.24
metabolism
CrtZ is one of ratelimiting enzymes for astaxanthin ((3S,3'S)-3,3'-dihydroxy-beta,beta-carotene-4,4'-dione) production
marine bacterium P99-3
1.14.15.24
metabolism
CrtZ is one of ratelimiting enzymes for astaxanthin ((3S,3'S)-3,3'-dihydroxy-beta,beta-carotene-4,4'-dione) production
Pantoea ananatis
1.14.15.24
metabolism
CrtZ is one of ratelimiting enzymes for astaxanthin ((3S,3'S)-3,3'-dihydroxy-beta,beta-carotene-4,4'-dione) production; CrtZ is one of ratelimiting enzymes for astaxanthin ((3S,3'S)-3,3'-dihydroxy-beta,beta-carotene-4,4'-dione) production
Paracoccus sp.
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.15.24
metabolism
CrtZ is one of ratelimiting enzymes for astaxanthin ((3S,3'S)-3,3'-dihydroxy-beta,beta-carotene-4,4'-dione) production
Brevundimonas sp.
1.14.15.24
metabolism
CrtZ is one of ratelimiting enzymes for astaxanthin ((3S,3'S)-3,3'-dihydroxy-beta,beta-carotene-4,4'-dione) production
marine bacterium P99-3
1.14.15.24
metabolism
CrtZ is one of ratelimiting enzymes for astaxanthin ((3S,3'S)-3,3'-dihydroxy-beta,beta-carotene-4,4'-dione) production
Pantoea ananatis
1.14.15.24
metabolism
CrtZ is one of ratelimiting enzymes for astaxanthin ((3S,3'S)-3,3'-dihydroxy-beta,beta-carotene-4,4'-dione) production
Paracoccus sp.