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Literature summary extracted from

  • Blasco, F.; Kauffmann, I.; Schmid, R.
    CYP175A1 from Thermus thermophilus HB27, the first beta-carotene hydroxylase of the P450 superfamily (2004), Appl. Microbiol. Biotechnol., 64, 671-674.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.24 expressed in Escherichia coli JM109 cells Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.24 Thermus thermophilus
-
-
-
1.14.15.24 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.24 beta-carotene + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Thermus thermophilus beta-cryptoxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.24 beta-carotene + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 beta-cryptoxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.24 beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2 overall reaction Thermus thermophilus zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O zeaxanthin is beta,beta-carotene-3,3'-diol ?
1.14.15.24 beta-carotene + 4 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + 2 O2 overall reaction Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 zeaxanthin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O zeaxanthin is beta,beta-carotene-3,3'-diol ?
1.14.15.24 beta-cryptoxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Thermus thermophilus zeaxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.24 beta-cryptoxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 zeaxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.24 echinenone + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Thermus thermophilus 3'-hydroxyechinenone + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.24 echinenone + 2 reduced ferredoxin [iron-sulfur] cluster + H+ + O2
-
 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 3'-hydroxyechinenone + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.15.24 CYP175A1
-
 Thermus thermophilus