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Literature summary extracted from

  • Sakamoto, Y.; Nakade, K.; Konno, N.
    An endo-{beta}-1,3-glucanase, GLU1, from Lentinula edodes fruiting body belongs to a new glycoside hydrolase family (2011), Appl. Environ. Microbiol., 77, 8350-8354.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.39 expression in Pichia pastoris Lentinula edodes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.39 25000
-
 x * 25000, SDS-PAGE, x * 26000, calculated Lentinula edodes
3.2.1.39 26000
-
 x * 25000, SDS-PAGE, x * 26000, calculated Lentinula edodes

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Lentinula edodes G9M5R4 Glu1 does not belong to existing GH families containing EC 3.2.1.39 glucanases
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.39 from fruiting body Lentinula edodes

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.39 fruit body
-
 Lentinula edodes
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.39 1.85
-
 pH 4.2, 37°C Lentinula edodes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 carboxymethyl curdlan + H2O 13.7% of the activity with laminarin Lentinula edodes ?
-
 ?
3.2.1.39 carboxymethyl pachyman + H2O 9.1% of the activity with laminarin Lentinula edodes ?
-
 ?
3.2.1.39 curdlan + H2O 9.1% of the activity with laminarin Lentinula edodes ?
-
 ?
3.2.1.39 laminarin + H2O endolytic depolymerization of glucans composed of beta-1,3-linked main chains Lentinula edodes D-glucose + laminarioligosaccharides
-
 ?
3.2.1.39 lentinan + H2O 4.4% of the activity with laminarin Lentinula edodes ?
-
 ?
3.2.1.39 additional information no substrate: beta-1,3-1,4-glucans such as barley glucan or lichenan, or beta-1,6 glucans such as pustulan Lentinula edodes ?
-
 ?
3.2.1.39 pachyman + H2O 14.9% of the activity with laminarin Lentinula edodes ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.39 ? x * 25000, SDS-PAGE, x * 26000, calculated Lentinula edodes

Synonyms

EC Number Synonyms Comment Organism
3.2.1.39 Glu1
-
 Lentinula edodes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.39 50
-
-
 Lentinula edodes

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.39 60
-
 30 min, complete inactivation Lentinula edodes

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.39 0.84
-
 Laminarin pH 4.2, 37°C Lentinula edodes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.39 4
-
-
 Lentinula edodes

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.39 3 9 4°C, stable for 20 h Lentinula edodes

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.39 Lentinula edodes isoelectric focusing
-
 3.9