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Literature summary extracted from

  • Jeon, J.H.; Kim, S.J.; Lee, H.S.; Cha, S.S.; Lee, J.H.; Yoon, S.H.; Koo, B.S.; Lee, C.M.; Choi, S.H.; Lee, S.H.; Kang, S.G.; Lee, J.H.
    A novel metagenome-derived carboxylesterase that hydrolyzes beta-lactam antibiotics (2011), Appl. Environ. Microbiol., 77, 7830-7836.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.1 expressed in Escherichia coli BL21(DE3) cells uncultured bacterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 cephaloridine competitive inhibitor of 4-nitrophenyl butyrate uncultured bacterium
3.1.1.1 cephalothin competitive inhibitor of 4-nitrophenyl butyrate uncultured bacterium
3.1.1.1 additional information EstU1 activity is not affected by the presence of EDTA (94% activity at 1 mM) uncultured bacterium
3.1.1.1 phenylmethylsulfonyl fluoride EstU1 loses approximately 70% of its activity in the presence of 1 mM phenylmethylsulfonyl fluoride uncultured bacterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.1 0.00603
-
 4-nitrophenyl butyrate at 35°C in 100 mM sodium phosphate (pH 7.0) uncultured bacterium
3.1.1.1 0.07849
-
 cefazolin at 35°C in 100 mM sodium phosphate (pH 7.0) uncultured bacterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.1 additional information EstU1 activity is not affected by the presence of Ca2+ (91% activity at 1 mM), Co2+ (89% activity at 1 mM), Cu2+ (88% activity at 1 mM), Fe2+ (84% activity at 1 mM), Mg2+ (91% activity at 1 mM), Mn2+ (74% activity at 1 mM), Ni2+ (89% activity at 1 mM), and Zn2+ (78% activity at 1 mM) uncultured bacterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 44000
-
 x * 44000, SDS-PAGE uncultured bacterium

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 uncultured bacterium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1 Talon metal affinity resin column chromatography and Superdex-75 gel filtration uncultured bacterium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.1 3.75
-
 using 4-nitrophenyl decanoate as substrate, at 35°C in 100 mM sodium phosphate (pH 7.0) uncultured bacterium
3.1.1.1 6.24
-
 using 4-nitrophenyl acetate as substrate, at 35°C in 100 mM sodium phosphate (pH 7.0) uncultured bacterium
3.1.1.1 7.46
-
 using 4-nitrophenyl octanoate as substrate, at 35°C in 100 mM sodium phosphate (pH 7.0) uncultured bacterium
3.1.1.1 11.78
-
 using 4-nitrophenyl hexanoate as substrate, at 35°C in 100 mM sodium phosphate (pH 7.0) uncultured bacterium
3.1.1.1 22.24
-
 using 4-nitrophenyl butyrate as substrate, at 35°C in 100 mM sodium phosphate (pH 7.0) uncultured bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 4-nitrophenyl acetate + H2O
-
 uncultured bacterium 4-nitrophenol + acetate
-
 ?
3.1.1.1 4-nitrophenyl butyrate + H2O highest activity uncultured bacterium 4-nitrophenol + butyrate
-
 ?
3.1.1.1 4-nitrophenyl decanoate + H2O
-
 uncultured bacterium 4-nitrophenol + decanoate
-
 ?
3.1.1.1 4-nitrophenyl hexanoate + H2O
-
 uncultured bacterium 4-nitrophenol + hexanoate
-
 ?
3.1.1.1 4-nitrophenyl octanoate + H2O
-
 uncultured bacterium 4-nitrophenol + octanoate
-
 ?
3.1.1.1 ampicillin + H2O
-
 uncultured bacterium ?
-
 ?
3.1.1.1 cefazolin + H2O
-
 uncultured bacterium (6R,7R)-7-amino-3-{[(5-methyl-1,3,4-thiadiazol-2-yl)sulfanyl]methyl}-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid + (1H-tetrazol-1-yl)acetic acid
-
 ?
3.1.1.1 cephaloridine + H2O
-
 uncultured bacterium (6R,7R)-7-amino-8-oxo-3-[(pyridin-1-ium-1-yl)methyl]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate + (thiophen-2-yl)acetic acid
-
 ?
3.1.1.1 cephalothin + H2O
-
 uncultured bacterium acetate + deacetylcephalothin
-
 ?
3.1.1.1 additional information no enzyme activity is detected toward 4-nitrophenyl laurate, 4-nitrophenyl myristate, 4-nitrophenyl palmitate, and 4-nitrophenyl stearate uncultured bacterium ?
-
 ?
3.1.1.1 nitrocefin + H2O i.e. 3-(2,4-dinitrosyl)-(6R,7R)-7-(2-thienylacetamido)-ceph-3-em-4-carboxylic acid uncultured bacterium ?
-
 ?
3.1.1.1 penicillin G + H2O
-
 uncultured bacterium 6-aminopenicillanic acid + phenylacetic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 ? x * 44000, SDS-PAGE uncultured bacterium

Synonyms

EC Number Synonyms Comment Organism
3.1.1.1 EstU1
-
 uncultured bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 45
-
-
 uncultured bacterium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.1 5 75
-
 uncultured bacterium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.1 0.000238
-
 cefazolin at 35°C in 100 mM sodium phosphate (pH 7.0) uncultured bacterium
3.1.1.1 15.72
-
 4-nitrophenyl butyrate at 35°C in 100 mM sodium phosphate (pH 7.0) uncultured bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 8.5
-
-
 uncultured bacterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.1 7.5 9.5
-
 uncultured bacterium

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.1.1 0.14
-
 cephalothin at 35°C in 100 mM sodium phosphate (pH 7.0) uncultured bacterium
3.1.1.1 0.215
-
 cephaloridine at 35°C in 100 mM sodium phosphate (pH 7.0) uncultured bacterium

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.1 0.003035
-
 cefazolin at 35°C in 100 mM sodium phosphate (pH 7.0) uncultured bacterium
3.1.1.1 2608
-
 4-nitrophenyl butyrate at 35°C in 100 mM sodium phosphate (pH 7.0) uncultured bacterium