EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.3.3 | His-tagged version expressed in Escherichia coli BL21(DE3)pLys | Lactiplantibacillus plantarum |
EC Number | General Stability | Organism |
---|---|---|
4.1.3.3 | 5-10% activity in the synthetic direction above pH 11.0 | Lactiplantibacillus plantarum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.3.3 | 1.8 | - |
N-acetylneuraminic acid | pH 7.0, 37°C | Lactiplantibacillus plantarum | |
4.1.3.3 | 19.9 | - |
pyruvate | pH 7.0, 37°C | Lactiplantibacillus plantarum | |
4.1.3.3 | 160 | - |
N-acetyl-D-mannosamine | pH 7.0, 37°C | Lactiplantibacillus plantarum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.3.3 | 37317 | - |
4 * 37317, HPLC/ESI/ion trap | Lactiplantibacillus plantarum |
4.1.3.3 | 132000 | - |
gel filtration | Lactiplantibacillus plantarum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.3.3 | Lactiplantibacillus plantarum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.3.3 | 100-kDa ultrafiltration step, 65°C heat shock step and Ni2+ chelating affinity chromatography | Lactiplantibacillus plantarum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.1.3.3 | 7.65 | - |
pH 7.0, 37°C | Lactiplantibacillus plantarum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.3.3 | N-acetylneuraminic acid | - |
Lactiplantibacillus plantarum | pyruvate + N-acetyl-D-mannosamine | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.3.3 | homotetramer | 4 * 37317, HPLC/ESI/ion trap | Lactiplantibacillus plantarum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.3.3 | N-Acetylneuraminate lyase | - |
Lactiplantibacillus plantarum |
4.1.3.3 | Sialic acid aldolase | - |
Lactiplantibacillus plantarum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.3.3 | 60 | - |
synthesis of N-acetylneuraminic acid | Lactiplantibacillus plantarum |
4.1.3.3 | 70 | - |
cleavage activity | Lactiplantibacillus plantarum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.3.3 | 60 | - |
80% cleavage activity after 48 hours | Lactiplantibacillus plantarum |
4.1.3.3 | 70 | - |
less than 10% after 8 hours | Lactiplantibacillus plantarum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.3.3 | 7 | 7.3 | for both directions | Lactiplantibacillus plantarum |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
4.1.3.3 | 5 | 9 | for both directions | Lactiplantibacillus plantarum |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
4.1.3.3 | 6.5 | 11 | about 80% activity (synthesis of N-acetylneuraminic acid) after 15 days at pH 8.0-10.0 and 37°C, 60% activity (synthesis of N-acetylneuraminic acid) after 15 days at pH 11.0 and 37°C | Lactiplantibacillus plantarum |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.3.3 | 0.03 | - |
N-acetyl-D-mannosamine | pH 7.0, 37°C | Lactiplantibacillus plantarum | |
4.1.3.3 | 0.11 | - |
pyruvate | pH 7.0, 37°C | Lactiplantibacillus plantarum | |
4.1.3.3 | 5.6 | - |
N-acetylneuraminic acid | pH 7.0, 37°C | Lactiplantibacillus plantarum |