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Literature summary extracted from

  • Krause, F.S.; Blombach, B.; Eikmanns, B.J.
    Metabolic engineering of Corynebacterium glutamicum for 2-ketoisovalerate production (2010), Appl. Environ. Microbiol., 76, 8053-8061.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.2.4.1 synthesis engineering of the wild type of Corynebacterium glutamicum for the growth-decoupled production of 2-ketoisovalerate from glucose by deletion of the aceE gene encoding the E1p subunit of the pyruvate dehydrogenase complex, deletion of the transaminase B gene ilvE, and additional overexpression of the ilvBNCD genes, encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase (AHAS), acetohydroxyacid isomeroreductase, and dihydroxyacid dehydratase. 2-Ketoisovalerate production is further improved by deletion of the pyruvate:quinone oxidoreductase gene pqo. In fed-batch fermentations at high cell densities, the newly constructed strains produce up to 188 mM (21.8 g/liter) 2-ketoisovalerate and showd a product yield of about 0.47 mol per mol (0.3 g/g) of glucose and a volumetric productivity of about 4.6 mM (0.53 g/liter) 2-ketoisovalerate per h in the overall production phase Corynebacterium glutamicum
2.2.1.6 synthesis engineering of the wild type of Corynebacterium glutamicum for the growth-decoupled production of 2-ketoisovalerate from glucose by deletion of the aceE gene encoding the E1p subunit of the pyruvate dehydrogenase complex, deletion of the transaminase B gene ilvE, and additional overexpression of the ilvBNCD genes, encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase (AHAS), acetohydroxyacid isomeroreductase, and dihydroxyacid dehydratase. 2-Ketoisovalerate production is further improved by deletion of the pyruvate:quinone oxidoreductase gene pqo. In fed-batch fermentations at high cell densities, the newly constructed strains produce up to 188 mM (21.8 g/liter) 2-ketoisovalerate and showd a product yield of about 0.47 mol per mol (0.3 g/g) of glucose and a volumetric productivity of about 4.6 mM (0.53 g/liter) 2-ketoisovalerate per h in the overall production phase Corynebacterium glutamicum

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.9 additional information engineering of wild-type Corynebacterium glutamicum for growth-decoupled production of 2-ketoisovalerate from glucose by deletion of the aceE gene encoding the E1p subunit of the pyruvate dehydrogenase complex, deletion of the transaminase B gene ilvE, and additional overexpression of the ilvBNCD genes, encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase, acetohydroxyacid isomeroreductase, and dihydroxyacid dehydratase. 2-Ketoisovalerate production is further improved by deletion of the pyruvate:quinone oxidoreductase gene pqo. In fed-batch fermentations at high cell densities, the constructed strains produce up to 188 mM 2-ketoisovalerate and show a product yield of about 0.47 mol per mol of glucose and a volumetric productivity of about 4.6 mM 2-ketoisovalerate per h in the overall production phase, overview Corynebacterium glutamicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.6 2-oxoisovalerate competitive Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.9 DL-2,3-dihydroxyisovalerate Corynebacterium glutamicum
-
2-oxo-isovalerate + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.2.4.1 Corynebacterium glutamicum Q8NNF6
-
-
1.2.4.1 Corynebacterium glutamicum DSM 20300 Q8NNF6
-
-
2.2.1.6 Corynebacterium glutamicum P42463 P42463, i.e. acetolactate synthase large subunit ilvB
-
2.2.1.6 Corynebacterium glutamicum DSM 20300 P42463 P42463, i.e. acetolactate synthase large subunit ilvB
-
4.2.1.9 Corynebacterium glutamicum
-
gene ilvD
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.9 DL-2,3-dihydroxyisovalerate
-
Corynebacterium glutamicum 2-oxo-isovalerate + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.2.4.1 aceE
-
Corynebacterium glutamicum
2.2.1.6 ilvB
-
Corynebacterium glutamicum
4.2.1.9 dihydroxyacid dehydratase
-
Corynebacterium glutamicum

General Information

EC Number General Information Comment Organism
4.2.1.9 metabolism dihydroxyacid dehydratase, a key enzyme involved in branched-chain amino acid biosynthesis, catalyses the synthesis of 2-oxoacids from dihydroxyacids Corynebacterium glutamicum