EC Number | Application | Comment | Organism |
---|---|---|---|
1.2.4.1 | synthesis | engineering of the wild type of Corynebacterium glutamicum for the growth-decoupled production of 2-ketoisovalerate from glucose by deletion of the aceE gene encoding the E1p subunit of the pyruvate dehydrogenase complex, deletion of the transaminase B gene ilvE, and additional overexpression of the ilvBNCD genes, encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase (AHAS), acetohydroxyacid isomeroreductase, and dihydroxyacid dehydratase. 2-Ketoisovalerate production is further improved by deletion of the pyruvate:quinone oxidoreductase gene pqo. In fed-batch fermentations at high cell densities, the newly constructed strains produce up to 188 mM (21.8 g/liter) 2-ketoisovalerate and showd a product yield of about 0.47 mol per mol (0.3 g/g) of glucose and a volumetric productivity of about 4.6 mM (0.53 g/liter) 2-ketoisovalerate per h in the overall production phase | Corynebacterium glutamicum |
2.2.1.6 | synthesis | engineering of the wild type of Corynebacterium glutamicum for the growth-decoupled production of 2-ketoisovalerate from glucose by deletion of the aceE gene encoding the E1p subunit of the pyruvate dehydrogenase complex, deletion of the transaminase B gene ilvE, and additional overexpression of the ilvBNCD genes, encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase (AHAS), acetohydroxyacid isomeroreductase, and dihydroxyacid dehydratase. 2-Ketoisovalerate production is further improved by deletion of the pyruvate:quinone oxidoreductase gene pqo. In fed-batch fermentations at high cell densities, the newly constructed strains produce up to 188 mM (21.8 g/liter) 2-ketoisovalerate and showd a product yield of about 0.47 mol per mol (0.3 g/g) of glucose and a volumetric productivity of about 4.6 mM (0.53 g/liter) 2-ketoisovalerate per h in the overall production phase | Corynebacterium glutamicum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.1.9 | additional information | engineering of wild-type Corynebacterium glutamicum for growth-decoupled production of 2-ketoisovalerate from glucose by deletion of the aceE gene encoding the E1p subunit of the pyruvate dehydrogenase complex, deletion of the transaminase B gene ilvE, and additional overexpression of the ilvBNCD genes, encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase, acetohydroxyacid isomeroreductase, and dihydroxyacid dehydratase. 2-Ketoisovalerate production is further improved by deletion of the pyruvate:quinone oxidoreductase gene pqo. In fed-batch fermentations at high cell densities, the constructed strains produce up to 188 mM 2-ketoisovalerate and show a product yield of about 0.47 mol per mol of glucose and a volumetric productivity of about 4.6 mM 2-ketoisovalerate per h in the overall production phase, overview | Corynebacterium glutamicum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.2.1.6 | 2-oxoisovalerate | competitive | Corynebacterium glutamicum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.9 | DL-2,3-dihydroxyisovalerate | Corynebacterium glutamicum | - |
2-oxo-isovalerate + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.4.1 | Corynebacterium glutamicum | Q8NNF6 | - |
- |
1.2.4.1 | Corynebacterium glutamicum DSM 20300 | Q8NNF6 | - |
- |
2.2.1.6 | Corynebacterium glutamicum | P42463 | P42463, i.e. acetolactate synthase large subunit ilvB | - |
2.2.1.6 | Corynebacterium glutamicum DSM 20300 | P42463 | P42463, i.e. acetolactate synthase large subunit ilvB | - |
4.2.1.9 | Corynebacterium glutamicum | - |
gene ilvD | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.9 | DL-2,3-dihydroxyisovalerate | - |
Corynebacterium glutamicum | 2-oxo-isovalerate + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.4.1 | aceE | - |
Corynebacterium glutamicum |
2.2.1.6 | ilvB | - |
Corynebacterium glutamicum |
4.2.1.9 | dihydroxyacid dehydratase | - |
Corynebacterium glutamicum |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.2.1.9 | metabolism | dihydroxyacid dehydratase, a key enzyme involved in branched-chain amino acid biosynthesis, catalyses the synthesis of 2-oxoacids from dihydroxyacids | Corynebacterium glutamicum |