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Literature summary extracted from

  • An, D.S.; Cui, C.H.; Lee, H.G.; Wang, L.; Kim, S.C.; Lee, S.T.; Jin, F.; Yu, H.; Chin, Y.W.; Lee, H.K.; Im, W.T.; Kim, S.G.
    Identification and characterization of a novel Terrabacter ginsenosidimutans sp. nov. beta-glucosidase that transforms ginsenoside Rb1 into the rare gypenosides XVII and LXXV (2010), Appl. Environ. Microbiol., 76, 5827-5836.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.21 expressed in Escherichia coli as a GST-tagged fusion protein Terrabacter ginsenosidimutans
3.2.1.191 expression in Escherichia coli Terrabacter ginsenosidimutans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 Hg2+ significant inhibition Terrabacter ginsenosidimutans
3.2.1.191 Hg2+ 10 mM, complete inhibition Terrabacter ginsenosidimutans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.14
-
 3-O-[beta-D-glucopyranosyl-(1-2)-beta-D-glucopyranosyl]-20-O-[beta-D-glucopyranosyl-(1-6)-beta-D-glucopyranosyl]-20(S)-protopanaxadiol pH 7.0, 37°C Terrabacter ginsenosidimutans
3.2.1.21 4.2
-
 4-nitrophenyl-beta-D-glucopyranoside pH 7.0, 37°C Terrabacter ginsenosidimutans
3.2.1.191 0.14
-
 ginsenoside Rb1 pH 7.0, 37°C Terrabacter ginsenosidimutans
3.2.1.191 4.2
-
 4-nitrophenyl beta-D-glucopyranoside pH 7.0, 37°C Terrabacter ginsenosidimutans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.191 additional information the enzyme does not require Mg2+, Mn2+, Co2+, Zn2+, Ca2+, or Cu2+ for activity Terrabacter ginsenosidimutans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 70000
-
 SDS-PAGE Terrabacter ginsenosidimutans
3.2.1.21 193000
-
 native protein, gel filtration Terrabacter ginsenosidimutans
3.2.1.191 70000
-
 3 * 70000, SDS-PAGE Terrabacter ginsenosidimutans
3.2.1.191 71140
-
 3 * 71140, SDS-PAGE Terrabacter ginsenosidimutans
3.2.1.191 193000
-
 gel filtration Terrabacter ginsenosidimutans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Terrabacter ginsenosidimutans
-
-
-
3.2.1.191 Terrabacter ginsenosidimutans D2KAT1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 using a glutathione-Sepharose 4B column Terrabacter ginsenosidimutans
3.2.1.191
-
 Terrabacter ginsenosidimutans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 2-nitrophenyl beta-D-glucopyranoside + H2O
-
 Terrabacter ginsenosidimutans 2-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 3-O-[beta-D-glucopyranosyl-(1-2)-beta-D-glucopyranosyl]-20-O-[beta-D-glucopyranosyl-(1-6)-beta-D-glucopyranosyl]-20(S)-protopanaxadiol + 2 H2O Conversion of ginsenoside Rb1 to gypenoside LXXV. BgpA catalyzes the hydrolysis of the two glucose moieties attached to the C-3 position of ginsenoside Rb1, and the outer glucose attached to the C-20 position. Cleavages occur in a defined order, with the outer glucose of C-3 cleaved first, followed by the inner glucose of C-3, and finally the outer glucose of C-20. BgpA selectively and sequentially converts ginsenoside Rb1 to the rare ginsenosides gypenoside XVII, gypenoside LXXV, and then C-K Terrabacter ginsenosidimutans 20-O-[beta-D-glucopyranosyl-(1-6)-beta-D-glucopyranosyl]-20(S)-protopanaxadiol + 2 D-glucose
-
 ?
3.2.1.21 3-O-[beta-D-glucopyranosyl-(1-2)-beta-D-glucopyranosyl]-20-O-[beta-D-glucopyranosyl-(1-6)-beta-D-glucopyranosyl]-20(S)-protopanaxadiol + 3 H2O Conversion of ginsenoside Rb1 to C-K. BgpA catalyzes the hydrolysis of the two glucose moieties attached to the C-3 position of ginsenoside Rb1, and the outer glucose attached to the C-20 position. Cleavages occur in a defined order, with the outer glucose of C-3 cleaved first, followed by the inner glucose of C-3, and finally the outer glucose of C-20. BgpA selectively and sequentially converts ginsenoside Rb1 to the rare ginsenosides gypenoside XVII, gypenoside LXXV, and then C-K Terrabacter ginsenosidimutans 20-O-(beta-D-glucopyranosyl)-20(S)-protopanaxadiol + 3 D-glucose
-
 ?
3.2.1.21 3-O-[beta-D-glucopyranosyl-(1-2)-beta-D-glucopyranosyl]-20-O-[beta-D-glucopyranosyl-(1-6)-beta-D-glucopyranosyl]-20(S)-protopanaxadiol + H2O Conversion of ginsenoside Rb1 to gypenoside XVII. BgpA catalyzes the hydrolysis of the two glucose moieties attached to the C-3 position of ginsenoside Rb1, and the outer glucose attached to the C-20 position. Cleavages occur in a defined order, with the outer glucose of C-3 is cleaved first, followed by the inner glucose of C-3, and finally the outer glucose of C-20. BgpA selectively and sequentially converts ginsenoside Rb1 to the rare ginsenosides gypenoside XVII, gypenoside LXXV, and then C-K Terrabacter ginsenosidimutans 3-O-beta-D-glucopyranosyl-20-O-[beta-D-glucopyranosyl-(1-6)-beta-D-glucopyranosyl]-20(S)-protopanaxadiol + D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Terrabacter ginsenosidimutans 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.191 2-nitrophenyl beta-D-glucopyranoside + H2O
-
 Terrabacter ginsenosidimutans 2-nitrophenol + D-glucopyranose
-
 ?
3.2.1.191 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Terrabacter ginsenosidimutans 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.191 ginsenoside Rb1 + H2O hydrolysis of 3-O-beta-D-(1->2)-glucopyranosyl bond Terrabacter ginsenosidimutans gypenoside XVII + D-glucopyranose
-
 ?
3.2.1.191 gypenoside XVII + H2O hydrolysis of 3-O-beta-D-glucopyranosyl bond Terrabacter ginsenosidimutans gypenoside LXXV + D-glucopyranose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 trimer SDS-PAGE, 3 * 70000 Terrabacter ginsenosidimutans
3.2.1.191 trimer 3 * 70000, SDS-PAGE Terrabacter ginsenosidimutans
3.2.1.191 trimer 3 * 71140, SDS-PAGE Terrabacter ginsenosidimutans

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 beta-glucosidase
-
 Terrabacter ginsenosidimutans
3.2.1.21 bgpA
-
 Terrabacter ginsenosidimutans
3.2.1.191 bgpA
-
 Terrabacter ginsenosidimutans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 37
-
 assay at Terrabacter ginsenosidimutans
3.2.1.21 45
-
 optimal temperature Terrabacter ginsenosidimutans
3.2.1.191 37
-
 assay at Terrabacter ginsenosidimutans
3.2.1.191 45
-
-
 Terrabacter ginsenosidimutans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 37 45 enzyme is stable at temperatures lower than 37°C, and about 32% of the activity is lost after incubation at 45°C for 30 min Terrabacter ginsenosidimutans
3.2.1.191 37
-
 the enzyme is stable at temperatures lower than 37°C Terrabacter ginsenosidimutans
3.2.1.191 45
-
 about 32% of the activity is lost after incubation at 45°C for 30 min Terrabacter ginsenosidimutans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 7
-
 assay at, optimum pH Terrabacter ginsenosidimutans
3.2.1.191 7
-
 in both sodium phosphate and McIlvaine buffers Terrabacter ginsenosidimutans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.21 5 10
-
 Terrabacter ginsenosidimutans
3.2.1.191 6 8 the enzyme retains over 82% of its optimal activity between pH values 6.0 and 8.0. It exhibits residual activity at pH 5.0, 9.0, and 10.0, and it shows no activity at pH 4.0 Terrabacter ginsenosidimutans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.21 4
-
 no activity Terrabacter ginsenosidimutans