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Literature summary extracted from

  • Ravanal, M.C.; Callegari, E.; Eyzaguirre, J.
    Novel bifunctional alpha-L-arabinofuranosidase/xylobiohydrolase (ABF3) from Penicillium purpurogenum (2010), Appl. Environ. Microbiol., 76, 5247-5253.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.37 0.65
-
 4-nitrophenyl alpha-L-arabinopyranoside pH 4.0, 30°C Talaromyces purpureogenus
3.2.1.37 12
-
 4-nitrophenyl beta-D-xyloside pH 4.0, 30°C Talaromyces purpureogenus
3.2.1.37 12
-
 4-nitrophenyl alpha-D-xylopyranoside in 50 mM sodium citrate buffer (pH 4.0), at 30°C Talaromyces purpureogenus
3.2.1.55 0.65
-
 4-nitrophenyl alpha-L-arabinofuranoside in 50 mM sodium citrate buffer (pH 4.0), at 30°C Talaromyces purpureogenus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.37 28000
-
 gel filtration Talaromyces purpureogenus
3.2.1.37 47305
-
 1 * 47305, mature protein, calculated from amino acid sequence Talaromyces purpureogenus
3.2.1.37 47305
-
 1 * 50700, SDS-PAGE, 1 * 47305, calculated Talaromyces purpureogenus
3.2.1.37 50700
-
 1 * 50700, deduced from SDS-PAGE Talaromyces purpureogenus
3.2.1.37 50700
-
 1 * 50700, SDS-PAGE, 1 * 47305, calculated Talaromyces purpureogenus
3.2.1.55 47305
-
 1 * 47305, mature protein, calculated from amino acid sequence Talaromyces purpureogenus
3.2.1.55 50700
-
 1 * 50700, deduced from SDS-PAGE Talaromyces purpureogenus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.55 debranched sugar beet arabinan + H2O Talaromyces purpureogenus
-
 L-arabinose + ? 1.35% L-arabinose liberated after 72 h incubation at 28°C ?
3.2.1.55 sugar beet arabinan + H2O Talaromyces purpureogenus
-
 arabinose + ? 0.82% L-arabinose liberated after 72 h incubation at 28°C ?
3.2.1.55 wheat flour arabinoxylan + H2O Talaromyces purpureogenus
-
 L-arabinose + ? 15.9% L-arabinose liberated after 72 h incubation at 28°C ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 Talaromyces purpureogenus C3W4Y2 bifunctional alpha-L-arabinofuranosidase/xylobiohydrolase
-
3.2.1.37 Talaromyces purpureogenus C3W4Y2 soft rot fungus
-
3.2.1.37 Talaromyces purpureogenus ATCC MYA-38 C3W4Y2 bifunctional alpha-L-arabinofuranosidase/xylobiohydrolase
-
3.2.1.37 Talaromyces purpureogenus ATCC MYA-38 C3W4Y2 soft rot fungus
-
3.2.1.55 Talaromyces purpureogenus C3W4Y2 soft rot fungus
-
3.2.1.55 Talaromyces purpureogenus ATCC MYA-38 C3W4Y2 soft rot fungus
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.37 glycoprotein
-
 Talaromyces purpureogenus
3.2.1.55 glycoprotein
-
 Talaromyces purpureogenus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.37
-
 Talaromyces purpureogenus
3.2.1.37 ammonium sulfate precipitation, DEAE-Sephadex gel filtration, SP Sepharose column chromatography and BioGel P-300 gel filtration Talaromyces purpureogenus
3.2.1.55 ammonium sulfate precipitation, DEAE-Sephadex gel filtration, SP Sepharose column chromatography and BioGel P-300 gel filtration Talaromyces purpureogenus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.37 89.5
-
 pH 4.0, 30°C, substrate 4-nitrophenyl alpha-L-arabinopyranoside Talaromyces purpureogenus
3.2.1.55 1.76
-
 crude extract, using 4-nitrophenyl alpha-L-arabinofuranoside as substrate, in 50 mM sodium citrate buffer (pH 4.0), at 30°C Talaromyces purpureogenus
3.2.1.55 89.5
-
 purified enzyme, using 4-nitrophenyl alpha-L-arabinofuranoside as substrate, in 50 mM sodium citrate buffer (pH 4.0), at 30°C Talaromyces purpureogenus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 4-nitrophenyl alpha-D-xylopyranoside + H2O
-
 Talaromyces purpureogenus 4-nitrophenol + D-xylopyranose
-
 ?
3.2.1.37 4-nitrophenyl alpha-D-xylopyranoside + H2O
-
 Talaromyces purpureogenus ATCC MYA-38 4-nitrophenol + D-xylopyranose
-
 ?
3.2.1.37 4-nitrophenyl alpha-L-arabinopyranoside + H2O
-
 Talaromyces purpureogenus 4-nitrophenol + alpha-L-arabinopyranose less than 2% activity with 4-nitrophenyl alpha-L-arabinopyranoside ?
3.2.1.37 4-nitrophenyl alpha-L-arabinopyranoside + H2O
-
 Talaromyces purpureogenus ATCC MYA-38 4-nitrophenol + alpha-L-arabinopyranose less than 2% activity with 4-nitrophenyl alpha-L-arabinopyranoside ?
3.2.1.37 4-nitrophenyl beta-D-xyloside + H2O
-
 Talaromyces purpureogenus 4-nitrophenol + beta-D-xylose
-
 ?
3.2.1.37 4-nitrophenyl beta-D-xyloside + H2O
-
 Talaromyces purpureogenus ATCC MYA-38 4-nitrophenol + beta-D-xylose
-
 ?
3.2.1.37 additional information less than 2% activity with 4-nitrophenyl alpha-L-arabinopyranoside, 2-nitrophenyl beta-D-xylopyranoside, 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-mannopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, 4-nitrophenyl beta-D-fucopyranoside, 4-nitrophenyl alpha-D-galactopyranoside, and 4-nitrophenyl beta-D-galctopyranoside. Only small amounts of xylose are released from oligosaccharides. Smaller oligosaccharides are produced when xylotriose through xylopentaose are used as substrates, suggesting a possible xylobiohydrolase activity. No transglycosylation is detected Talaromyces purpureogenus ?
-
 ?
3.2.1.37 additional information less than 2% activity with 4-nitrophenyl alpha-L-arabinopyranoside, 2-nitrophenyl beta-D-xylopyranoside, 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-mannopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, 4-nitrophenyl beta-D-fucopyranoside, 4-nitrophenyl alpha-D-galactopyranoside, and 4-nitrophenyl beta-D-galctopyranoside. Only small amounts of xylose are released from oligosaccharides. Smaller oligosaccharides are produced when xylotriose through xylopentaose are used as substrates, suggesting a possible xylobiohydrolase activity. No transglycosylation is detected Talaromyces purpureogenus ATCC MYA-38 ?
-
 ?
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Talaromyces purpureogenus 4-nitrophenol + L-arabinofuranose
-
 ?
3.2.1.55 debranched sugar beet arabinan + H2O
-
 Talaromyces purpureogenus L-arabinose + ? 1.35% L-arabinose liberated after 72 h incubation at 28°C ?
3.2.1.55 additional information the enzyme does not hydrolyze arabinooligosaccharides, 4-nitrophenyl alpha-L-arabinopyranoside, o-nitrophenyl beta-D-xylopyranoside, 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-mannopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, 4-nitrophenyl beta-D-fucopyranoside, 4-nitrophenyl alpha-D-galactopyranoside, and 4-nitrophenyl beta-D-galactopyranoside (less than 2% activity with each substrate) Talaromyces purpureogenus ?
-
 ?
3.2.1.55 additional information the enzyme does not hydrolyze arabinooligosaccharides, 4-nitrophenyl alpha-L-arabinopyranoside, o-nitrophenyl beta-D-xylopyranoside, 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-mannopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, 4-nitrophenyl beta-D-fucopyranoside, 4-nitrophenyl alpha-D-galactopyranoside, and 4-nitrophenyl beta-D-galactopyranoside (less than 2% activity with each substrate) Talaromyces purpureogenus ATCC MYA-38 ?
-
 ?
3.2.1.55 sugar beet arabinan + H2O
-
 Talaromyces purpureogenus arabinose + ? 0.82% L-arabinose liberated after 72 h incubation at 28°C ?
3.2.1.55 wheat flour arabinoxylan + H2O
-
 Talaromyces purpureogenus L-arabinose + ? 15.9% L-arabinose liberated after 72 h incubation at 28°C ?

Subunits

EC Number Subunits Comment Organism
3.2.1.37 monomer 1 * 47305, mature protein, calculated from amino acid sequence Talaromyces purpureogenus
3.2.1.37 monomer 1 * 50700, deduced from SDS-PAGE Talaromyces purpureogenus
3.2.1.37 monomer 1 * 50700, SDS-PAGE, 1 * 47305, calculated Talaromyces purpureogenus
3.2.1.55 monomer 1 * 47305, mature protein, calculated from amino acid sequence Talaromyces purpureogenus
3.2.1.55 monomer 1 * 50700, deduced from SDS-PAGE Talaromyces purpureogenus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.37 ABF3
-
 Talaromyces purpureogenus
3.2.1.37 ABF3 bifunctional enzyme having both alpha-L-arabinofuranosidase and xylobiohydrolase functionalities Talaromyces purpureogenus
3.2.1.37 alpha-L-arabinofuranosidase 3
-
 Talaromyces purpureogenus
3.2.1.37 alpha-L-arabinofuranoside arabinofuranohydrolase
-
 Talaromyces purpureogenus
3.2.1.37 arabinofuranosidase 3 bifunctional enzyme having both alpha-L-arabinofuranosidase and xylobiohydrolase functionalities Talaromyces purpureogenus
3.2.1.55 ABF3 bifunctional enzyme having both alpha-L-arabinofuranosidase and xylobiohydrolase functionalities Talaromyces purpureogenus
3.2.1.55 alpha-L-arabinofuranoside arabinofuranohydrolase
-
 Talaromyces purpureogenus
3.2.1.55 arabinofuranosidase 3 bifunctional enzyme having both alpha-L-arabinofuranosidase and xylobiohydrolase functionalities Talaromyces purpureogenus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.37 50
-
-
 Talaromyces purpureogenus
3.2.1.55 50
-
-
 Talaromyces purpureogenus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.37 35
-
 30 min, 35% loss of activity Talaromyces purpureogenus
3.2.1.37 35 50 the enzyme loses 40% of its activity at 35°C and 90% at 50°C after 30 min of incubation Talaromyces purpureogenus
3.2.1.37 50
-
 30 min, 90% loss of activity Talaromyces purpureogenus
3.2.1.55 35 50 the enzyme loses 40% of its activity at 35°C and 90% at 50°C after 30 min of incubation Talaromyces purpureogenus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.37 2.73
-
 4-nitrophenyl alpha-L-arabinopyranoside pH 4.0, 30°C Talaromyces purpureogenus
3.2.1.37 253.3
-
 4-nitrophenyl alpha-D-xylopyranoside in 50 mM sodium citrate buffer (pH 4.0), at 30°C Talaromyces purpureogenus
3.2.1.37 253.3
-
 4-nitrophenyl beta-D-xyloside pH 4.0, 30°C Talaromyces purpureogenus
3.2.1.55 2.73
-
 4-nitrophenyl alpha-L-arabinofuranoside in 50 mM sodium citrate buffer (pH 4.0), at 30°C Talaromyces purpureogenus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.37 5
-
-
 Talaromyces purpureogenus
3.2.1.55 5
-
-
 Talaromyces purpureogenus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.37 4.21
-
 4-nitrophenyl alpha-L-arabinopyranoside pH 4.0, 30°C Talaromyces purpureogenus
3.2.1.37 21.1
-
 4-nitrophenyl beta-D-xyloside pH 4.0, 30°C Talaromyces purpureogenus
3.2.1.37 21.7
-
 4-nitrophenyl alpha-D-xylopyranoside in 50 mM sodium citrate buffer (pH 4.0), at 30°C Talaromyces purpureogenus
3.2.1.55 4.17
-
 4-nitrophenyl alpha-L-arabinofuranoside in 50 mM sodium citrate buffer (pH 4.0), at 30°C Talaromyces purpureogenus