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Literature summary extracted from

  • Wang, W.Z.; Morohoshi, T.; Ikenoya, M.; Someya, N.; Ikeda, T.
    AiiM, a novel class of N-acylhomoserine lactonase from the leaf-associated bacterium Microbacterium testaceum (2010), Appl. Environ. Microbiol., 76, 2524-2530.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.81 gene aiiM, cloning from the genomic library, DNA and amino acid sequence determination and analysis, expression as a maltose binding protein-fusion protein in Escherichia coli strain DH5alpha Microbacterium testaceum

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.81 additional information expression of Microbacterium testaceum AiiM in the plant pathogen Pectobacterium carotovorum subsp. carotovorum reduces the pectinase activity markedly and attenuated soft rot symptoms on Solanum tuberosum tuber slices Microbacterium testaceum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.81 74000
-
 x * 74000, recombinant MBP-fusion enzyme, SDS-PAGE Microbacterium testaceum

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.81 Microbacterium testaceum C6L862 isolated from the leaf surface of Solanum tuberosum, gene aiiM
-
3.1.1.81 Microbacterium testaceum StLB037 C6L862 isolated from the leaf surface of Solanum tuberosum, gene aiiM
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.81 additional information strain StLB037 grows at temperatures ranging from 10°C to 60°C, reaching its optimum temperature at 37°C Microbacterium testaceum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.81 L-homoserine lactone + H2O 20% of the activity with N-decanoyl-L-homoserine lactone Microbacterium testaceum L-homoserine
-
 ?
3.1.1.81 L-homoserine lactone + H2O 20% of the activity with N-decanoyl-L-homoserine lactone Microbacterium testaceum StLB037 L-homoserine
-
 ?
3.1.1.81 additional information poor activity with N-butyryl-L-homoserine lactone Microbacterium testaceum ?
-
 ?
3.1.1.81 additional information poor activity with N-butyryl-L-homoserine lactone Microbacterium testaceum StLB037 ?
-
 ?
3.1.1.81 N-decanoyl-L-homoserine lactone + H2O best substrate Microbacterium testaceum N-decanoyl-L-homoserine
-
 ?
3.1.1.81 N-decanoyl-L-homoserine lactone + H2O best substrate Microbacterium testaceum StLB037 N-decanoyl-L-homoserine
-
 ?
3.1.1.81 N-dodecanoyl-L-homoserine lactone + H2O
-
 Microbacterium testaceum N-dodecanoyl-L-homoserine
-
 ?
3.1.1.81 N-dodecanoyl-L-homoserine lactone + H2O
-
 Microbacterium testaceum StLB037 N-dodecanoyl-L-homoserine
-
 ?
3.1.1.81 N-octanoyl-L-homoserine lactone + H2O
-
 Microbacterium testaceum N-octanoyl-L-homoserine
-
 ?
3.1.1.81 N-octanoyl-L-homoserine lactone + H2O
-
 Microbacterium testaceum StLB037 N-octanoyl-L-homoserine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.81 ? x * 74000, recombinant MBP-fusion enzyme, SDS-PAGE Microbacterium testaceum

Synonyms

EC Number Synonyms Comment Organism
3.1.1.81 AHL lactonase
-
 Microbacterium testaceum
3.1.1.81 AiiM
-
 Microbacterium testaceum
3.1.1.81 More the enzyme belongs to the alpha/beta hydrolase fold family Microbacterium testaceum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.81 37
-
 assay at Microbacterium testaceum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.81 15 80 over 80% of maximal activity within the range of 20-60°C Microbacterium testaceum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.81 8
-
-
 Microbacterium testaceum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.81 4.2 8 no activity below pH 4.2, maximal activity at pH 8.0 Microbacterium testaceum

General Information

EC Number General Information Comment Organism
3.1.1.81 additional information the AHL-degrading enzyme catalyzes the lactone ring opening of N-acyl-L-homoserine lactones by hydrolyzing the lactones Microbacterium testaceum