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Literature summary extracted from

  • Ku, B.; Jeong, J.C.; Mijts, B.N.; Schmidt-Dannert, C.; Dordick, J.S.
    Preparation, characterization, and optimization of an in vitro C30 carotenoid pathway (2005), Appl. Environ. Microbiol., 71, 6578-6583.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.5.1.96 synthesis construction of an in vitro C30 carotenoid pathway based on recombinant Escherichia coli farnesyl diphosphate synthase and 4,4'-diapophytoene synthase. The 4,4'-diapophytoene product is unstabel in aqueous buffer. In situ extraction using an aqueous-organic two-phase system results in a 100% conversion of isopentenyl diphosphate and dimethylallyl diphosphate into 4,4'-diapophytoene synthase Staphylococcus aureus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.96 expression in Escherichia coli Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.96 0.0012
-
 (2E,6E)-farnesyl diphosphate pH 7.7, 37°C Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.96 Staphylococcus aureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.96 recombinant protein Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.96 2 (2E,6E)-farnesyl diphosphate
-
 Staphylococcus aureus cis-4,4'-diapophytoene + 2 diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.96 DAP synthase
-
 Staphylococcus aureus