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Literature summary extracted from

  • di Gennaro, P.; Colmegna, A.; Galli, E.; Sello, G.; Pelizzoni, F.; Bestetti, G.
    A new biocatalyst for production of optically pure aryl epoxides by styrene monooxygenase from Pseudomonas fluorescens ST (1999), Appl. Environ. Microbiol., 65, 2794-2797.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.14.14.11 synthesis the enzyme converts aryl ethenyl compounds to the corresponding epoxides in optically pure forms and with good yields Pseudomonas fluorescens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.11 expression in Escherichia coli Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.11 styrene + FADH2 + O2 Pseudomonas fluorescens
-
 (S)-2-phenyloxirane + FAD + H2O
-
 ?
1.14.14.11 styrene + FADH2 + O2 Pseudomonas fluorescens ST
-
 (S)-2-phenyloxirane + FAD + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.11 Pseudomonas fluorescens
-
-
-
1.14.14.11 Pseudomonas fluorescens ST
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.11 (2E)-3-phenylprop-2-en-1-ol + FADH2 + O2
-
 Pseudomonas fluorescens (3-phenyloxiran-2-yl)methanol + 1-phenylpropane-1,2,3-triol + FAD + H2O
-
 ?
1.14.14.11 (2E)-3-phenylprop-2-en-1-ol + FADH2 + O2
-
 Pseudomonas fluorescens ST (3-phenyloxiran-2-yl)methanol + 1-phenylpropane-1,2,3-triol + FAD + H2O
-
 ?
1.14.14.11 (2E)-3-phenylprop-2-en-1-yl acetate + FADH2 + O2
-
 Pseudomonas fluorescens (3-phenyloxiran-2-yl)methyl acetate + 2,3-dihydroxy-3-phenylpropyl acetate + FAD + H2O
-
 ?
1.14.14.11 (2E)-3-phenylprop-2-en-1-yl acetate + FADH2 + O2
-
 Pseudomonas fluorescens ST (3-phenyloxiran-2-yl)methyl acetate + 2,3-dihydroxy-3-phenylpropyl acetate + FAD + H2O
-
 ?
1.14.14.11 1,2-dihydronaphthalene + FADH2 + O2
-
 Pseudomonas fluorescens (1R,2R)-1,2,3,4-tetrahydronaphthalene-1,2-diol + FAD + H2O
-
 ?
1.14.14.11 1,2-dihydronaphthalene + FADH2 + O2
-
 Pseudomonas fluorescens ST (1R,2R)-1,2,3,4-tetrahydronaphthalene-1,2-diol + FAD + H2O
-
 ?
1.14.14.11 2-ethenylpyridine + FADH2 + O2
-
 Pseudomonas fluorescens 2-(oxiran-2-yl)pyridine + FAD + H2O
-
 ?
1.14.14.11 4-ethenylpyridine + FADH2 + O2
-
 Pseudomonas fluorescens 4-(oxiran-2-yl)pyridine + FAD + H2O
-
 ?
1.14.14.11 methyl (2E)-3-phenylprop-2-enoate + FADH2 + O2
-
 Pseudomonas fluorescens methyl 3-phenyloxirane-2-carboxylate + methyl 2,3-dihydroxy-3-phenylpropanoate + FAD + H2O
-
 ?
1.14.14.11 additional information no product with 2H-chromen-2-one Pseudomonas fluorescens ?
-
 ?
1.14.14.11 additional information no product with 2H-chromen-2-one Pseudomonas fluorescens ST ?
-
 ?
1.14.14.11 naphthalene + FADH2 + O2
-
 Pseudomonas fluorescens (1R,2R)-1,2-dihydronaphthalene-1,2-diol + FAD + H2O trace amount ?
1.14.14.11 prop-1-en-2-ylbenzene + FADH2 + O2
-
 Pseudomonas fluorescens 2-phenylpropane-1,2-diol + FAD + H2O
-
 ?
1.14.14.11 styrene + FADH2 + O2
-
 Pseudomonas fluorescens (S)-2-phenyloxirane + FAD + H2O
-
 ?
1.14.14.11 styrene + FADH2 + O2
-
 Pseudomonas fluorescens ST (S)-2-phenyloxirane + FAD + H2O
-
 ?