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Literature summary extracted from

  • Nam, E.S.; Kim, M.S.; Lee, H.B.; Ahn, J.K.
    Beta-glycosidase of Thermus thermophilus KNOUC202: gene and biochemical properties of the enzyme expressed in Escherichia coli (2010), Appl. Biochem. Microbiol., 46, 515-524.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.21 expressed in Escherichia coli Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.05
-
 4-nitrophenyl-beta-D-glucopyranoside pH 5.4, 75°C Thermus thermophilus
3.2.1.21 4.84
-
 4-nitrophenyl-beta-D-galactopyranoside pH 5.4, 75°C Thermus thermophilus
3.2.1.21 6.05
-
 2-nitrophenyl-beta-D-galactopyranoside pH 5.4, 75°C Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 48660
-
 deduced from cDNA Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Thermus thermophilus Q0GA07
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 using gel filtration and ion-exchange chromatography Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Thermus thermophilus 2-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-fucopyranoside + H2O
-
 Thermus thermophilus 4-nitrophenol + beta-D-fucopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Thermus thermophilus 4-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Thermus thermophilus 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-lactopyranoside + H2O
-
 Thermus thermophilus 4-nitrophenol + D-lactose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 monomer gel filtration Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 beta-glucosidase
-
 Thermus thermophilus
3.2.1.21 KNOUC202beta-gly
-
 Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 75
-
 assay at Thermus thermophilus
3.2.1.21 90
-
 optimal temperature Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 80
-
 enzyme shows high thermostability not losing its activity at 80°C for 2 h in 0.05 M Na-phosphate buffer of pH 6.8 with Tm of 100.0°C in 0.02 M Tris-HCl buffer of pH 8.2 Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.21 32.3
-
 4-nitrophenyl-beta-D-glucopyranoside pH 5.4, 75°C Thermus thermophilus
3.2.1.21 167.4
-
 4-nitrophenyl-beta-D-galactopyranoside pH 5.4, 75°C Thermus thermophilus
3.2.1.21 288.3
-
 2-nitrophenyl-beta-D-galactopyranoside pH 5.4, 75°C Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 5.4
-
 optimal pH Thermus thermophilus
3.2.1.21 7
-
 assay at Thermus thermophilus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.21 34.6
-
 4-nitrophenyl-beta-D-galactopyranoside pH 5.4, 75°C Thermus thermophilus
3.2.1.21 47.7
-
 2-nitrophenyl-beta-D-galactopyranoside pH 5.4, 75°C Thermus thermophilus
3.2.1.21 700.6
-
 4-nitrophenyl-beta-D-glucopyranoside pH 5.4, 75°C Thermus thermophilus