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Literature summary extracted from

  • Lin, F.P.; Ma, H.Y.; Lin, H.J.; Liu, S.M.; Tzou, W.S.
    Biochemical characterization of two truncated forms of amylopullulanase from Thermoanaerobacterium saccharolyticum NTOU1 to identify its enzymatically active region (2011), Appl. Biochem. Biotechnol., 165, 1047-1056.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.41 expressed in Escherichia coli Rosetta (DE3) pLysS cells Thermoanaerobacterium saccharolyticum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.41 Thermoanaerobacterium saccharolyticum C5IGQ7
-
-
3.2.1.41 Thermoanaerobacterium saccharolyticum NTOU1 C5IGQ7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.41 BD TALON spin column chromatography, gel filtration Thermoanaerobacterium saccharolyticum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.41 amylopectin + H2O
-
Thermoanaerobacterium saccharolyticum maltotriose + maltose + ?
-
?
3.2.1.41 amylopectin + H2O
-
Thermoanaerobacterium saccharolyticum NTOU1 maltotriose + maltose + ?
-
?
3.2.1.41 amylose + H2O
-
Thermoanaerobacterium saccharolyticum maltotriose + maltose
-
?
3.2.1.41 amylose + H2O
-
Thermoanaerobacterium saccharolyticum NTOU1 maltotriose + maltose
-
?
3.2.1.41 glycogen + H2O
-
Thermoanaerobacterium saccharolyticum maltotriose + maltose
-
?
3.2.1.41 additional information the enzyme cleaves both alpha-1,4- and alpha-1,6-glycosidic linkages in starch, pullulan, amylopectin, and other related oligosaccharides Thermoanaerobacterium saccharolyticum ?
-
?
3.2.1.41 additional information the enzyme cleaves both alpha-1,4- and alpha-1,6-glycosidic linkages in starch, pullulan, amylopectin, and other related oligosaccharides Thermoanaerobacterium saccharolyticum NTOU1 ?
-
?
3.2.1.41 pullulan + H2O
-
Thermoanaerobacterium saccharolyticum maltotriose + ?
-
?
3.2.1.41 pullulan + H2O
-
Thermoanaerobacterium saccharolyticum NTOU1 maltotriose + ?
-
?
3.2.1.41 soluble starch + H2O
-
Thermoanaerobacterium saccharolyticum maltotriose + maltose
-
?
3.2.1.41 soluble starch + H2O
-
Thermoanaerobacterium saccharolyticum NTOU1 maltotriose + maltose
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.41 amylopullulanase
-
Thermoanaerobacterium saccharolyticum
3.2.1.41 Apu
-
Thermoanaerobacterium saccharolyticum
3.2.1.41 ApuK885 truncated enzyme form Thermoanaerobacterium saccharolyticum
3.2.1.41 ApuM957 truncated enzyme form Thermoanaerobacterium saccharolyticum
3.2.1.41 pullulanase type II
-
Thermoanaerobacterium saccharolyticum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.41 70
-
-
Thermoanaerobacterium saccharolyticum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.41 574.7
-
soluble starch truncated enzyme form ApuK855, at pH 6.0 and 70°C Thermoanaerobacterium saccharolyticum
3.2.1.41 877.9
-
soluble starch truncated enzyme form ApuM957, at pH 6.0 and 70°C Thermoanaerobacterium saccharolyticum
3.2.1.41 1751
-
pullulan truncated enzyme form ApuM957, at pH 5.0 and 70°C Thermoanaerobacterium saccharolyticum
3.2.1.41 1828
-
pullulan truncated enzyme form ApuK855, at pH 5.0 and 70°C Thermoanaerobacterium saccharolyticum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.41 5
-
optimum pH for pullulanase activity Thermoanaerobacterium saccharolyticum
3.2.1.41 6
-
optimum pH for amylase activity Thermoanaerobacterium saccharolyticum