EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.41 | expressed in Escherichia coli Rosetta (DE3) pLysS cells | Thermoanaerobacterium saccharolyticum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.41 | Thermoanaerobacterium saccharolyticum | C5IGQ7 | - |
- |
3.2.1.41 | Thermoanaerobacterium saccharolyticum NTOU1 | C5IGQ7 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.41 | BD TALON spin column chromatography, gel filtration | Thermoanaerobacterium saccharolyticum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.41 | amylopectin + H2O | - |
Thermoanaerobacterium saccharolyticum | maltotriose + maltose + ? | - |
? | |
3.2.1.41 | amylopectin + H2O | - |
Thermoanaerobacterium saccharolyticum NTOU1 | maltotriose + maltose + ? | - |
? | |
3.2.1.41 | amylose + H2O | - |
Thermoanaerobacterium saccharolyticum | maltotriose + maltose | - |
? | |
3.2.1.41 | amylose + H2O | - |
Thermoanaerobacterium saccharolyticum NTOU1 | maltotriose + maltose | - |
? | |
3.2.1.41 | glycogen + H2O | - |
Thermoanaerobacterium saccharolyticum | maltotriose + maltose | - |
? | |
3.2.1.41 | additional information | the enzyme cleaves both alpha-1,4- and alpha-1,6-glycosidic linkages in starch, pullulan, amylopectin, and other related oligosaccharides | Thermoanaerobacterium saccharolyticum | ? | - |
? | |
3.2.1.41 | additional information | the enzyme cleaves both alpha-1,4- and alpha-1,6-glycosidic linkages in starch, pullulan, amylopectin, and other related oligosaccharides | Thermoanaerobacterium saccharolyticum NTOU1 | ? | - |
? | |
3.2.1.41 | pullulan + H2O | - |
Thermoanaerobacterium saccharolyticum | maltotriose + ? | - |
? | |
3.2.1.41 | pullulan + H2O | - |
Thermoanaerobacterium saccharolyticum NTOU1 | maltotriose + ? | - |
? | |
3.2.1.41 | soluble starch + H2O | - |
Thermoanaerobacterium saccharolyticum | maltotriose + maltose | - |
? | |
3.2.1.41 | soluble starch + H2O | - |
Thermoanaerobacterium saccharolyticum NTOU1 | maltotriose + maltose | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.41 | amylopullulanase | - |
Thermoanaerobacterium saccharolyticum |
3.2.1.41 | Apu | - |
Thermoanaerobacterium saccharolyticum |
3.2.1.41 | ApuK885 | truncated enzyme form | Thermoanaerobacterium saccharolyticum |
3.2.1.41 | ApuM957 | truncated enzyme form | Thermoanaerobacterium saccharolyticum |
3.2.1.41 | pullulanase type II | - |
Thermoanaerobacterium saccharolyticum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.41 | 70 | - |
- |
Thermoanaerobacterium saccharolyticum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.41 | 574.7 | - |
soluble starch | truncated enzyme form ApuK855, at pH 6.0 and 70°C | Thermoanaerobacterium saccharolyticum | |
3.2.1.41 | 877.9 | - |
soluble starch | truncated enzyme form ApuM957, at pH 6.0 and 70°C | Thermoanaerobacterium saccharolyticum | |
3.2.1.41 | 1751 | - |
pullulan | truncated enzyme form ApuM957, at pH 5.0 and 70°C | Thermoanaerobacterium saccharolyticum | |
3.2.1.41 | 1828 | - |
pullulan | truncated enzyme form ApuK855, at pH 5.0 and 70°C | Thermoanaerobacterium saccharolyticum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.41 | 5 | - |
optimum pH for pullulanase activity | Thermoanaerobacterium saccharolyticum |
3.2.1.41 | 6 | - |
optimum pH for amylase activity | Thermoanaerobacterium saccharolyticum |