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Literature summary extracted from

  • Li, L.; Jiang, X.; Guan, H.; Wang, P.; Guo, H.
    Three alginate lyases from marine bacterium Pseudomonas fluorescens HZJ216: Purification and characterization (2011), Appl. Biochem. Biotechnol., 164, 305-317.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.3 Ba2+ 50 mM, isoform A 89%, isoform B 44%, isoform C 42% of initial activity Pseudomonas fluorescens
4.2.2.3 Co2+ 50 mM, isoform A 67%, isoform B 66%, isoform C 29% of initial activity Pseudomonas fluorescens
4.2.2.3 Fe2+ 50 mM, no residual activity; complete inhibition of all isozymes at 50 mM Pseudomonas fluorescens
4.2.2.3 Fe3+
-
 Pseudomonas fluorescens
4.2.2.3 Zn2+ 50 mM, isoform A 10%, isoform B 14%, isoform C 12% of initial activity Pseudomonas fluorescens
4.2.2.11 Ba2+ 50 mM, isoform A 89%, isoform B 44%, isoform C 42% of initial activity Pseudomonas fluorescens
4.2.2.11 Co2+ 50 mM, isoform A 67%, isoform B 66%, isoform C 29% of initial activity Pseudomonas fluorescens
4.2.2.11 Fe2+ 50 mM, no residual activity Pseudomonas fluorescens
4.2.2.11 Zn2+ 50 mM, isoform A 10%, isoform B 14%, isoform C 12% of initial activity Pseudomonas fluorescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.3 Ca2+ activates Pseudomonas fluorescens
4.2.2.3 Ca2+ 50 mM, isoform A 117%, isoform B 131% of initial activity Pseudomonas fluorescens
4.2.2.3 Cu2+ activates Pseudomonas fluorescens
4.2.2.3 K+ activates Pseudomonas fluorescens
4.2.2.3 K+ 50 mM, isoform A 132%, isoform B 110% of initial activity Pseudomonas fluorescens
4.2.2.3 Mg2+ activates Pseudomonas fluorescens
4.2.2.3 Mg2+ 50 mM, isoform A 116%, isoform B 121%, isoform C 114% of initial activity Pseudomonas fluorescens
4.2.2.3 additional information no effect by Li+ at 50 mM Pseudomonas fluorescens
4.2.2.3 Na+ is essential for the activity of all the three lyases Pseudomonas fluorescens
4.2.2.3 NaCl activates at 1.5% Pseudomonas fluorescens
4.2.2.11 Ca2+ 50 mM, isoform A 117%, isoform B 131% of initial activity Pseudomonas fluorescens
4.2.2.11 K+ 50 mM, isoform A 132%, isoform B 110% of initial activity Pseudomonas fluorescens
4.2.2.11 Mg2+ 50 mM, isoform A 116%, isoform B 121%, isoform C 114% of initial activity Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.3 23000
-
 x * 60250, alginate lyase A, SDS-PAGE, x * 36000, alginate lyase B, SDS-PAGE, x * 23000, alginate lyase C, SDS-PAGE Pseudomonas fluorescens
4.2.2.3 23000
-
 x * 60250, isoform A, x * 36000, isoform B, x * 23000, isoform C, SDS-PAGE Pseudomonas fluorescens
4.2.2.3 36000
-
 x * 60250, alginate lyase A, SDS-PAGE, x * 36000, alginate lyase B, SDS-PAGE, x * 23000, alginate lyase C, SDS-PAGE Pseudomonas fluorescens
4.2.2.3 36000
-
 x * 60250, isoform A, x * 36000, isoform B, x * 23000, isoform C, SDS-PAGE Pseudomonas fluorescens
4.2.2.3 60250
-
 x * 60250, alginate lyase A, SDS-PAGE, x * 36000, alginate lyase B, SDS-PAGE, x * 23000, alginate lyase C, SDS-PAGE Pseudomonas fluorescens
4.2.2.3 60250
-
 x * 60250, isoform A, x * 36000, isoform B, x * 23000, isoform C, SDS-PAGE Pseudomonas fluorescens
4.2.2.11 23000
-
 x * 60250, isoform A, x * 36000, isoform B, x * 23000, isoform C, SDS-PAGE Pseudomonas fluorescens
4.2.2.11 36000
-
 x * 60250, isoform A, x * 36000, isoform B, x * 23000, isoform C, SDS-PAGE Pseudomonas fluorescens
4.2.2.11 60250
-
 x * 60250, isoform A, x * 36000, isoform B, x * 23000, isoform C, SDS-PAGE Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.2.3 additional information Pseudomonas fluorescens alginate lyase A degrades M and G blocks, and the enzyme activity acting on M block is much more than that of G block, while for alginate lyase B, the enzyme activities on M block are slightly higher than that of G block ?
-
 ?
4.2.2.3 additional information Pseudomonas fluorescens HZJ216 alginate lyase A degrades M and G blocks, and the enzyme activity acting on M block is much more than that of G block, while for alginate lyase B, the enzyme activities on M block are slightly higher than that of G block ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.3 Pseudomonas fluorescens
-
-
-
4.2.2.3 Pseudomonas fluorescens
-
 isolated from brown seaweed in the Yellow Sea of China
-
4.2.2.3 Pseudomonas fluorescens HZJ216
-
-
-
4.2.2.3 Pseudomonas fluorescens HZJ216
-
 isolated from brown seaweed in the Yellow Sea of China
-
4.2.2.11 Pseudomonas fluorescens
-
-
-
4.2.2.11 Pseudomonas fluorescens HZJ216
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.3 native alginate lyases, by acetone precipitation, anion exchange chromatography, and gel filtration Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.3 21.77
-
 alginate lyase C, substrate with M blocks, pH 7.0, 30°C Pseudomonas fluorescens
4.2.2.3 129
-
 alginate lyase C, substrate with G blocks, pH 7.0, 30°C Pseudomonas fluorescens
4.2.2.3 330
-
 alginate lyase B, substrate with G blocks, pH 7.0, 30°C Pseudomonas fluorescens
4.2.2.3 382
-
 alginate lyase B, substrate with M blocks, pH 7.0, 30°C Pseudomonas fluorescens
4.2.2.3 382
-
 isoform B, substrate poly(beta-D-mannuronate), pH 7.0, 30°C Pseudomonas fluorescens
4.2.2.3 390
-
 alginate lyase A, substrate with G blocks, pH 7.0, 30°C Pseudomonas fluorescens
4.2.2.3 467
-
 isoform B, substrate sodium alginate, pH 7.0, 30°C Pseudomonas fluorescens
4.2.2.3 651
-
 alginate lyase A, substrate with M blocks, pH 7.0, 30°C Pseudomonas fluorescens
4.2.2.3 651
-
 isoform A, substrate poly(beta-D-mannuronate), pH 7.0, 30°C Pseudomonas fluorescens
4.2.2.3 653
-
 isoform A, substrate sodium alginate, pH 7.0, 30°C Pseudomonas fluorescens
4.2.2.3 1123
-
 extracellular enzyme, pH 7.0, 30°C Pseudomonas fluorescens
4.2.2.11 233
-
 isoform C, substrate sodium alginate, pH 7.0, 30°C Pseudomonas fluorescens
4.2.2.11 330
-
 isoform B, substrate poly(alpha-L-guluronate), pH 7.0, 30°C Pseudomonas fluorescens
4.2.2.11 390
-
 isoform A, substrate poly(alpha-L-guluronate), pH 7.0, 30°C Pseudomonas fluorescens
4.2.2.11 467
-
 isoform B, substrate sodium alginate, pH 7.0, 30°C Pseudomonas fluorescens
4.2.2.11 653
-
 isoform A, substrate sodium alginate, pH 7.0, 30°C Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.3 alpha-L-mannuronyl linkage in alginate alginate lyases A and B have the activities for both M and G blocks. For isoform A, the enzyme activity acting on M block is much more than that of G block, for alginate lyase B, the enzyme activity on M block is slightly higher than that on G block and there is no obvious substrate specificity difference between them Pseudomonas fluorescens ?
-
 ?
4.2.2.3 alpha-L-mannuronyl linkage in alginate alginate lyases A and B have the activities for both M and G blocks. For isoform A, the enzyme activity acting on M block is much more than that of G block, for alginate lyase B, the enzyme activity on M block is slightly higher than that on G block and there is no obvious substrate specificity difference between them Pseudomonas fluorescens HZJ216 ?
-
 ?
4.2.2.3 additional information alginate lyase A degrades M and G blocks, and the enzyme activity acting on M block is much more than that of G block, while for alginate lyase B, the enzyme activities on M block are slightly higher than that of G block Pseudomonas fluorescens ?
-
 ?
4.2.2.3 additional information alginate lyase A degrades M and G blocks, and the enzyme activity acting on M block is much more than that of G block, while for alginate lyase B, the enzyme activities on M block are slightly higher than that of G block Pseudomonas fluorescens HZJ216 ?
-
 ?
4.2.2.3 poly(beta-(1->4)-D-mannuronate)
-
 Pseudomonas fluorescens ?
-
 ?
4.2.2.3 poly(beta-(1->4)-D-mannuronate)
-
 Pseudomonas fluorescens HZJ216 ?
-
 ?
4.2.2.11 alpha-L-guluronosyl linkage in alginate alginate lyase isoform C has the specificity for G block while alginate lyases A and B have the activities for both M and G blocks. For isoform A, the enzyme activity acting on M block is much more than that of G block, for alginate lyase B, the enzyme activity on M block is slightly higher than that on G block and there is no obvious substrate specificity difference between them Pseudomonas fluorescens ?
-
 ?
4.2.2.11 alpha-L-guluronosyl linkage in alginate alginate lyase isoform C has the specificity for G block while alginate lyases A and B have the activities for both M and G blocks. For isoform A, the enzyme activity acting on M block is much more than that of G block, for alginate lyase B, the enzyme activity on M block is slightly higher than that on G block and there is no obvious substrate specificity difference between them Pseudomonas fluorescens HZJ216 ?
-
 ?
4.2.2.11 poly(alpha-L-guluronate)
-
 Pseudomonas fluorescens ?
-
 ?
4.2.2.11 poly(alpha-L-guluronate)
-
 Pseudomonas fluorescens HZJ216 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.3 ? x * 60250, alginate lyase A, SDS-PAGE, x * 36000, alginate lyase B, SDS-PAGE, x * 23000, alginate lyase C, SDS-PAGE Pseudomonas fluorescens
4.2.2.3 ? x * 60250, isoform A, x * 36000, isoform B, x * 23000, isoform C, SDS-PAGE Pseudomonas fluorescens
4.2.2.11 ? x * 60250, isoform A, x * 36000, isoform B, x * 23000, isoform C, SDS-PAGE Pseudomonas fluorescens

Synonyms

EC Number Synonyms Comment Organism
4.2.2.3 alginate lyase A
-
 Pseudomonas fluorescens
4.2.2.3 alginate lyase B
-
 Pseudomonas fluorescens
4.2.2.3 alginate lyase C
-
 Pseudomonas fluorescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.3 3.5
-
-
 Pseudomonas fluorescens
4.2.2.3 35
-
-
 Pseudomonas fluorescens
4.2.2.11 3.5
-
-
 Pseudomonas fluorescens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.2.3 25
-
 isoform C, 54% of maximum activity Pseudomonas fluorescens
4.2.2.3 25 60 activity range, the lyases possess more than 60% activity in the range of 25°C to 40°C except alginate lyase C at 25°C which is 53.89% activity, but the activities decrease above 40°C Pseudomonas fluorescens
4.2.2.3 25 40 isoforms A, B, more than 60% of maximum activity Pseudomonas fluorescens
4.2.2.11 25
-
 isoform C, 54% of maximum activity Pseudomonas fluorescens
4.2.2.11 25 40 isoforms A, B, more than 60% of maximum activity Pseudomonas fluorescens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.3 35
-
 30 min, isoform B 59% residual activity, isoform C 55% residual activity Pseudomonas fluorescens
4.2.2.3 40
-
 isoform A, 30 min, 70% residual activity Pseudomonas fluorescens
4.2.2.11 35
-
 30 min, isoform B 59% residual activity, isoform C 55% residual activity Pseudomonas fluorescens
4.2.2.11 40
-
 isoform A, 30 min, 70% residual activity Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.3 7
-
-
 Pseudomonas fluorescens
4.2.2.11 7
-
-
 Pseudomonas fluorescens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.3 3 11 activity range, profile, overview Pseudomonas fluorescens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.3 5 7 alginate lyase C, stable Pseudomonas fluorescens
4.2.2.3 5 7 isoform C Pseudomonas fluorescens
4.2.2.3 5 9 alginate lyase A and B, stable Pseudomonas fluorescens
4.2.2.3 5 9 isoforms A and B Pseudomonas fluorescens
4.2.2.11 5 7 isoform C Pseudomonas fluorescens
4.2.2.11 5 9 isoforms A and B Pseudomonas fluorescens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.2.3 Pseudomonas fluorescens alginate lyase A, isoelectri focusing
-
 4
4.2.2.3 Pseudomonas fluorescens isoform A, isoelectric focusing
-
 4
4.2.2.3 Pseudomonas fluorescens alginate lyase B, isoelectri focusing
-
 4.36
4.2.2.3 Pseudomonas fluorescens isoform B, isoelectric focusing
-
 4.4
4.2.2.3 Pseudomonas fluorescens alginate lyase C, isoelectri focusing
-
 4.59
4.2.2.3 Pseudomonas fluorescens isoform C, isoelectric focusing
-
 4.6
4.2.2.11 Pseudomonas fluorescens isoform A, isoelectric focusing
-
 4
4.2.2.11 Pseudomonas fluorescens isoform B, isoelectric focusing
-
 4.4
4.2.2.11 Pseudomonas fluorescens isoform C, isoelectric focusing
-
 4.6