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Literature summary extracted from

  • Lee, K.M.; Joo, A.R.; Jeya, M.; Lee, K.M.; Moon, H.J.; Lee, J.K.
    Production and characterization of cellobiohydrolase from a novel strain of Penicillium purpurogenum KJS506 (2011), Appl. Biochem. Biotechnol., 163, 25-39.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.91 2-mercaptoethanol 136.5% activity at 0.5 mM Talaromyces purpureogenus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.91 Co2+ 33.7% inhibition at 1 mM Talaromyces purpureogenus
3.2.1.91 EDTA 24.6% inhibition at 1 mM Talaromyces purpureogenus
3.2.1.91 Fe2+ complete inhibition at 1 mM Talaromyces purpureogenus
3.2.1.91 Hg2+ complete inhibition at 1 mM Talaromyces purpureogenus
3.2.1.91 Zn2+ 21.2% inhibition at 1 mM Talaromyces purpureogenus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.91 1.1
-
 4-nitrophenyl beta-D-cellobioside at pH 5.0, 60°C Talaromyces purpureogenus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.91 60000
-
 1 * 60000, SDS-PAGE Talaromyces purpureogenus
3.2.1.91 61000
-
 gel filtration Talaromyces purpureogenus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.91 Talaromyces purpureogenus
-
-
-
3.2.1.91 Talaromyces purpureogenus KJS506
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.91 DEAE-Sepharose column chromatography, Mono Q column chromatography, and Sephacryl S-300 gel filtration Talaromyces purpureogenus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.91 2.6
-
 crude enzyme, pH 5.0, 60°C Talaromyces purpureogenus
3.2.1.91 10.8
-
 after4.2fold purification, pH 5.0, 60°C Talaromyces purpureogenus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.91 4-nitrophenyl beta-D-cellobioside + H2O best substrate Talaromyces purpureogenus 4-nitrophenol + cellobiose
-
 ?
3.2.1.91 4-nitrophenyl beta-D-cellobioside + H2O best substrate Talaromyces purpureogenus KJS506 4-nitrophenol + cellobiose
-
 ?
3.2.1.91 avicel + H2O
-
 Talaromyces purpureogenus ?
-
 ?
3.2.1.91 avicel + H2O
-
 Talaromyces purpureogenus KJS506 ?
-
 ?
3.2.1.91 cellopentaose + H2O
-
 Talaromyces purpureogenus cellobiose + ? cellobiose is the major product ?
3.2.1.91 cellopentaose + H2O
-
 Talaromyces purpureogenus KJS506 cellobiose + ? cellobiose is the major product ?
3.2.1.91 cellotetraose + H2O
-
 Talaromyces purpureogenus cellobiose + ? cellobiose is the major product ?
3.2.1.91 filter paper + H2O
-
 Talaromyces purpureogenus ?
-
 ?
3.2.1.91 filter paper + H2O
-
 Talaromyces purpureogenus KJS506 ?
-
 ?
3.2.1.91 additional information does not hydrolyze carboxymethylcellulose Talaromyces purpureogenus ?
-
 ?
3.2.1.91 additional information does not hydrolyze carboxymethylcellulose Talaromyces purpureogenus KJS506 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.91 monomer 1 * 60000, SDS-PAGE Talaromyces purpureogenus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.91 CBH
-
 Talaromyces purpureogenus
3.2.1.91 cellobiohydrolase
-
 Talaromyces purpureogenus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.91 60
-
 activity decreases rapidly at temperatures over 60°C Talaromyces purpureogenus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.91 60
-
 the enzyme shows a half-life of 72 h, 46 h, 22 h, 4 h, and 0.5 h at 30°C, 40°C, 50°C, 60°C, and 70 °C, respectively Talaromyces purpureogenus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.91 5
-
-
 Talaromyces purpureogenus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.91 4 7 82% and 79% of the maximum activity at pH 4.0 and and pH 6.0 Talaromyces purpureogenus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.91 Talaromyces purpureogenus isoelectric focusing
-
 4.8