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Literature summary extracted from

  • Liu, X.; Xu, J.H.; Pan, J.; Zhao, J.
    Efficient production of (S)-naproxen with (R)-substrate recycling using an overexpressed carboxylesterase BsE-NP01 (2010), Appl. Biochem. Biotechnol., 162, 1574-1584.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.1 expressed in Escherichia coli BL21 cells Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 (S)-naproxen nearly 80% of the initial activity is lost when 2.5 mM of (S)-naproxen is added to the reaction system Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Bacillus subtilis D0EPY0
-
-
3.1.1.1 Bacillus subtilis ECU0554 D0EPY0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 (S)-naproxen methyl ester + H2O
-
 Bacillus subtilis (S)-naproxen + methanol i.e. (+)-(S)-2-(6-methoxy-2-naphthyl)propionic acid, 98% enantiomeric excess ?
3.1.1.1 4-nitrophenyl acetate + H2O about 30% activity compared to 4-nitrophenyl butyrate Bacillus subtilis 4-nitrophenol + acetate
-
 ?
3.1.1.1 4-nitrophenyl acetate + H2O about 30% activity compared to 4-nitrophenyl butyrate Bacillus subtilis ECU0554 4-nitrophenol + acetate
-
 ?
3.1.1.1 4-nitrophenyl butyrate + H2O highest activity (100%) Bacillus subtilis 4-nitrophenol + butyrate
-
 ?
3.1.1.1 4-nitrophenyl butyrate + H2O highest activity (100%) Bacillus subtilis ECU0554 4-nitrophenol + butyrate
-
 ?
3.1.1.1 4-nitrophenyl caproate + H2O about 55% activity compared to 4-nitrophenyl butyrate Bacillus subtilis 4-nitrophenol + caproate
-
 ?
3.1.1.1 4-nitrophenyl decanoate + H2O about 30% activity compared to 4-nitrophenyl butyrate Bacillus subtilis 4-nitrophenol + decanoate
-
 ?
3.1.1.1 4-nitrophenyl dodecanoate + H2O about 20% activity compared to 4-nitrophenyl butyrate Bacillus subtilis 4-nitrophenol + dodecanoate
-
 ?
3.1.1.1 4-nitrophenyl octanoate + H2O about 45% activity compared to 4-nitrophenyl butyrate Bacillus subtilis 4-nitrophenol + octanoate
-
 ?
3.1.1.1 4-nitrophenyl octanoate + H2O about 45% activity compared to 4-nitrophenyl butyrate Bacillus subtilis ECU0554 4-nitrophenol + octanoate
-
 ?
3.1.1.1 4-nitrophenyl propionate + H2O about 70% activity compared to 4-nitrophenyl butyrate Bacillus subtilis 4-nitrophenol + propionate
-
 ?
3.1.1.1 4-nitrophenyl propionate + H2O about 70% activity compared to 4-nitrophenyl butyrate Bacillus subtilis ECU0554 4-nitrophenol + propionate
-
 ?
3.1.1.1 additional information no activity with 4-nitrophenyl myristate and 4-nitrophenyl palmitate Bacillus subtilis ?
-
 ?
3.1.1.1 additional information no activity with 4-nitrophenyl myristate and 4-nitrophenyl palmitate Bacillus subtilis ECU0554 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 ? x * about 32000, SDS-PAGE Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
3.1.1.1 BsE-NP01
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 50
-
-
 Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 8.5
-
-
 Bacillus subtilis