EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.3 | expressed in Escherichia coli BL21(DE3) cells | Bacillus sp. (in: Bacteria) |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.3 | Cu2+ | complete inhibition at 10 mM | Bacillus sp. (in: Bacteria) | |
3.1.1.3 | Ni2+ | 78% inhibition at 10 mM | Bacillus sp. (in: Bacteria) | |
3.1.1.3 | phenylmethylsulfonyl fluoride | complete inhibition at 10 mM | Bacillus sp. (in: Bacteria) | |
3.1.1.3 | Zn2+ | 86% inhibition at 10 mM | Bacillus sp. (in: Bacteria) |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.3 | Co2+ | the activity is enhanced more than twice at 1 mM CoCl2 | Bacillus sp. (in: Bacteria) |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 22800 | - |
x * 22800, calculated from amino acid sequence | Bacillus sp. (in: Bacteria) |
3.1.1.3 | 23000 | - |
x * 23000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.3 | Bacillus sp. (in: Bacteria) | - |
- |
- |
3.1.1.3 | Bacillus sp. (in: Bacteria) HH-01 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.3 | Ni-NTA column chromatography | Bacillus sp. (in: Bacteria) |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.3 | 1-naphthyl octanoate + H2O | - |
Bacillus sp. (in: Bacteria) | 1-naphthol + octanoate | - |
? | |
3.1.1.3 | 4-nitrophenyl butyrate + H2O | - |
Bacillus sp. (in: Bacteria) | 4-nitrophenol + butyrate | - |
? | |
3.1.1.3 | 4-nitrophenyl butyrate + H2O | - |
Bacillus sp. (in: Bacteria) HH-01 | 4-nitrophenol + butyrate | - |
? | |
3.1.1.3 | 4-nitrophenyl caprylate + H2O | at 30°C, the enzyme prefers C8 synthetic substrate (4-nitrophenyl caprylate)to shorter or longer substrates | Bacillus sp. (in: Bacteria) | 4-nitrophenol + caprylate | - |
? | |
3.1.1.3 | 4-nitrophenyl caprylate + H2O | at 30°C, the enzyme prefers C8 synthetic substrate (4-nitrophenyl caprylate)to shorter or longer substrates | Bacillus sp. (in: Bacteria) HH-01 | 4-nitrophenol + caprylate | - |
? | |
3.1.1.3 | 4-nitrophenyl laurate + H2O | - |
Bacillus sp. (in: Bacteria) | 4-nitrophenol + laurate | - |
? | |
3.1.1.3 | 4-nitrophenyl laurate + H2O | - |
Bacillus sp. (in: Bacteria) HH-01 | 4-nitrophenol + laurate | - |
? | |
3.1.1.3 | 4-nitrophenyl palmitate + H2O | - |
Bacillus sp. (in: Bacteria) | 4-nitrophenol + palmitate | - |
? | |
3.1.1.3 | 4-nitrophenyl palmitate + H2O | - |
Bacillus sp. (in: Bacteria) HH-01 | 4-nitrophenol + palmitate | - |
? | |
3.1.1.3 | 4-nitrophenyl stearate + H2O | - |
Bacillus sp. (in: Bacteria) | 4-nitrophenol + stearate | - |
? | |
3.1.1.3 | triacylglycerol + H2O | the strain HH-01 degrades 1.0% (v/v) edible oil in nutrient broth, almost completely within 10 days | Bacillus sp. (in: Bacteria) | diacylglycerol + a carboxylate | - |
? | |
3.1.1.3 | triacylglycerol + H2O | the strain HH-01 degrades 1.0% (v/v) edible oil in nutrient broth, almost completely within 10 days | Bacillus sp. (in: Bacteria) HH-01 | diacylglycerol + a carboxylate | - |
? | |
3.1.1.3 | trilinolein + H2O | the enzyme shows preferential hydrolysis of the 1(3)-position ester bond in trilinolein | Bacillus sp. (in: Bacteria) | dilinolein + linoleate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.3 | ? | x * 23000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
3.1.1.3 | ? | x * 22800, calculated from amino acid sequence | Bacillus sp. (in: Bacteria) |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.3 | LipA | - |
Bacillus sp. (in: Bacteria) |
3.1.1.3 | lipase | - |
Bacillus sp. (in: Bacteria) |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 30 | - |
- |
Bacillus sp. (in: Bacteria) |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 30 | - |
the enzyme is stable up to 30°C | Bacillus sp. (in: Bacteria) |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 8 | 9 | - |
Bacillus sp. (in: Bacteria) |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 4 | 11 | - |
Bacillus sp. (in: Bacteria) |
EC Number | Organism | Comment | Expression |
---|---|---|---|
3.1.1.3 | Bacillus sp. (in: Bacteria) | the lipA gene is down-regulated in the stationary phase | down |
3.1.1.3 | Bacillus sp. (in: Bacteria) | the lipA gene is constitutively transcribed during the exponential growth phase | up |