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Literature summary extracted from

  • Singh, D.; Pannier, A.; Zempleni, J.
    Identification of holocarboxylase synthetase chromatin binding sites in human mammary cell lines using the DNA adenine methyltransferase identification technology (2011), Anal. Biochem., 413, 55-59.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.1.1.72 medicine DNA adenine methyltransferase identification is a useful technology to map holocarboxylase synthetase binding sites in human chromatin Escherichia coli
6.3.4.10 analysis fusion of full-length HCS to DNA adenine methyltransferase Dam and subsequent transfection into breast cancer cells MCF-7 and normal breast cells MCF-10A for identification of chromatin binding sites Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.72 expressed in MCF-7 cells and in MCF-10A cells Escherichia coli
6.3.4.10 fusion of full-length HCS to DNA adenine methyltransferase Dam and subsequent transfection into breast cancer cells MCF-7 and normal breast cells MCF-10A Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.72 S-adenosyl-L-methionine + DNA adenine Escherichia coli
-
 S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.72 Escherichia coli
-
-
-
6.3.4.10 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.72 S-adenosyl-L-methionine + DNA adenine
-
 Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methyladenine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.72 Dam
-
 Escherichia coli
2.1.1.72 DNA adenine methyltransferase
-
 Escherichia coli
6.3.4.10 HCS
-
 Homo sapiens
6.3.4.10 Holocarboxylase synthetase
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
6.3.4.10 physiological function identification of HCS docking sites in chromatin. 4 out of 15 docking sites overlap between MCF-7 and MCF-10A cells, i.e. inositol polyphosphate-5-phosphatase A, corticotropin hormone precursor, ribosome biogenesis regulatory protein, and leptin precursor Homo sapiens