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Literature summary extracted from

  • Buga, M.; Ibrahim, S.; Nok, A.
    Partially purified polygalacturonase from Aspergillus niger (SA6) (2010), Afr. J. Biotechnol., 9, 8944-8954.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.2.1.67 stability studies of the enzyme at different pH values after 24 h of incubation at room temperature give the highest percentage residual activities as follows: 23.01% at pH 4.5 in acetate, 28.32% at pH 7.0 in phosphate, and 22.13% at pH 7.5 in Tris-HCl Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.15 35000
-
 x * 35000, SDS-PAGE Aspergillus niger
3.2.1.67 35000
-
 1 * 40000 + 1 * 35000, dimeric protein consisting of both endo- and exo-polygalacturonase activities, SDS-PAGE Aspergillus niger
3.2.1.67 40000
-
 1 * 40000 + 1 * 35000, dimeric protein consisting of both endo- and exo-polygalacturonase activities, SDS-PAGE Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.15 Aspergillus niger
-
-
-
3.2.1.15 Aspergillus niger SA6
-
-
-
3.2.1.67 Aspergillus niger
-
-
-
3.2.1.67 Aspergillus niger SA6
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.15 native enzyme 9fold by ultrafiltration, ammonium sulfate fractionation, ion exchange chromatography, and gel filtration Aspergillus niger
3.2.1.67 ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Sephadex G-75 gel filtration Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.15 246
-
 partially purified enzyme, pH 4.5, 40°C Aspergillus niger
3.2.1.67 26.99
-
 crude enzyme, at pH 4.5 and 40°C Aspergillus niger
3.2.1.67 246
-
 after 9.1fold purification, at pH 4.5 and 40°C Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.67 citric pectin + H2O
-
 Aspergillus niger D-galacturonic acid + ?
-
 ?
3.2.1.67 citric pectin + H2O
-
 Aspergillus niger SA6 D-galacturonic acid + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.15 ? x * 35000, SDS-PAGE Aspergillus niger
3.2.1.67 heterodimer 1 * 40000 + 1 * 35000, dimeric protein consisting of both endo- and exo-polygalacturonase activities, SDS-PAGE Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.2.1.15 endo PG
-
 Aspergillus niger
3.2.1.67 exoPG
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.15 40
-
-
 Aspergillus niger
3.2.1.67 40
-
-
 Aspergillus niger

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.67 30 50
-
 Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.67 30 70 about 55% activity at 30-40°C, about 45% activity at 50°C, about 25% activity at 60°C, about 15% activity at 70°C Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.15 4.5
-
-
 Aspergillus niger
3.2.1.67 4.5
-
-
 Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.67 4 5
-
 Aspergillus niger