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Literature summary extracted from

  • Garrabou, X.; Joglar, J.; Parella, T.; Crehuet, R.; Bujons, J.; Clapés, P.
    Redesign of the phosphate binding site of L-rhamnulose-1-phosphate aldolase towards a dihydroxyacetone dependent aldolase (2011), Adv. Synth. Catal., 353, 89-99.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.19 expressed in Escherichia coli strain M-15 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
4.1.2.19 N29D the mutant shows 5.3% of the wild type activity, the mutation increases by 3fold the Vappmax of aldol addition reactions of dihydroxyacetone to other aldehyde acceptors rather than the natural L-lactaldehyde Escherichia coli
4.1.2.19 N32D the mutant shows 0.1% of the wild type activity Escherichia coli
4.1.2.19 S116D the mutant shows 0.2% of the wild type activity Escherichia coli
4.1.2.19 S75D the mutant shows 0.1% of the wild type activity Escherichia coli
4.1.2.19 T115D completely inactive Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.19 phosphate the aldol addition of dihydroxyacetobe is strongly inhibited by phosphate Escherichia coli
4.1.2.19 sulfate
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.19 0.6
-
 dihydroxyacetone phosphate wild type enzyme, apparent value, at pH 7.0 and 25°C Escherichia coli
4.1.2.19 7
-
 dihydroxyacetone phosphate mutant enzyme N29D, apparent value, at pH 7.0 and 25°C Escherichia coli
4.1.2.19 1125
-
 dihydroxyacetone wild type enzyme, apparent value, at pH 7.0 and 25°C Escherichia coli
4.1.2.19 1339
-
 dihydroxyacetone mutant enzyme N29D, apparent value, at pH 7.0 and 25°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.19 Escherichia coli P32169
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.19 HR 16/40 afinity column chromatography Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.19 0.002
-
 mutant enzyme S75D, using L-rhamnulose-1-phosphate as substrate, at pH 7.0 and 25°C Escherichia coli
4.1.2.19 0.005
-
 mutant enzyme N32D, using L-rhamnulose-1-phosphate as substrate, at pH 7.0 and 25°C Escherichia coli
4.1.2.19 0.006
-
 mutant enzyme S116D, using L-rhamnulose-1-phosphate as substrate, at pH 7.0 and 25°C Escherichia coli
4.1.2.19 0.2
-
 mutant enzyme N29D, using L-rhamnulose-1-phosphate as substrate, at pH 7.0 and 25°C Escherichia coli
4.1.2.19 3.8
-
 wild type enzyme, using L-rhamnulose-1-phosphate as substrate, at pH 7.0 and 25°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.19 (benzyloxy)acetaldehyde + dihydroxyacetone favored direction of reaction Escherichia coli (3R,4S)-5-(benzyloxy)-1,3,4-trihydroxypentan-2-one + (3R,4R)-5-(benzyloxy)-1,3,4-trihydroxypentan-2-one
-
 r
4.1.2.19 benzyl (2-oxoethyl)carbamate + dihydroxyacetone favored direction of reaction Escherichia coli benzyl [(2S,3R)-2,3,5-trihydroxy-4-oxopentyl]carbamate + benzyl [(2R,3R)-2,3,5-trihydroxy-4-oxopentyl]carbamate
-
 r
4.1.2.19 benzyl [(2R)-1-oxopropan-2-yl]carbamate + dihydroxyacetone favored direction of reaction Escherichia coli benzyl [(2R,3S,4R)-3,4,6-trihydroxy-5-oxohexan-2-yl]carbamate + benzyl [(2R,3R,4R)-3,4,6-trihydroxy-5-oxohexan-2-yl]carbamate
-
 r
4.1.2.19 dihydroxyacetone + L-lactaldehyde favored direction of reaction Escherichia coli L-rhamnulose 1-phosphate
-
 r
4.1.2.19 dihydroxyacetone phosphate + L-lactaldehyde
-
 Escherichia coli ?
-
 ?
4.1.2.19 dihydroxyacetone phosphate + L-lactaldehyde favored direction of reaction Escherichia coli L-rhamnulose 1-phosphate
-
 r
4.1.2.19 L-rhamnulose 1-phosphate
-
 Escherichia coli dihydroxyacetone phosphate + L-lactaldehyde
-
 r
4.1.2.19 N-formylglycinal + dihydroxyacetone
-
 Escherichia coli N-[(2S,3R)-2,3,5-trihydroxy-4-oxopentyl]formamide + N-[(2R,3R)-2,3,5-trihydroxy-4-oxopentyl]formamide
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.1.2.19 L-Rhamnulose-1-phosphate aldolase
-
 Escherichia coli
4.1.2.19 RhuA
-
 Escherichia coli

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.1.2.19 0.5
-
 wild type enzyme, at pH 7.0 and 25°C Escherichia coli phosphate