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Literature summary extracted from
- Cloning, expression, purification and kinetics of trehalose-6-phosphate phosphatase of filarial parasite Brugia malayi (2011), Acta Trop., 119, 151-159.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.1.3.12 | drug development | Bm-TPP bodes well as a rational drug target against lymphatic filariasis | Brugia malayi |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.3.12 | DNA and amino acid sequence determination and analysis, expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) and release into the soluble fraction with a natively folded conformation | Brugia malayi |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.12 | acetate | no activity in Tris-citrate and only minimal activity in acetate buffer | Brugia malayi |  |
| 3.1.3.12 | citrate | no activity in Tris-citrate and only minimal activity in acetate buffer | Brugia malayi | |
| 3.1.3.12 | EDTA | - |
Brugia malayi | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.12 | Mg2+ | required, the enzyme is specific for Mg2+ as a cofactor and most of the other divalent cations are either slightly effective or totally ineffective in replacing Mg2+ | Brugia malayi | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.12 | 60000 | - |
x * 60000, recombinant His6-tagged SDS-PAGE | Brugia malayi |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.12 | Brugia malayi | A8NS89 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.3.12 | recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography | Brugia malayi |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.3.12 | additional information | Bm-TPP is expressed in all the lifestages of the parasite Brugia malayi. Adult worms are obtained by lavaging peritoneal cavity of jirds inoculated earlier with Brugia malayi infective larvae isolated from laboratory infected Aedes aegypti | Brugia malayi | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.12 | additional information | the enzyme is specific for trehalose 6-phosphate | Brugia malayi | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.3.12 | ? | x * 60000, recombinant His6-tagged SDS-PAGE | Brugia malayi |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.3.12 | More | Bm-TPP is a member of the HAD-like hydrolase super family II based on the conserved motifs required for catalytic reaction | Brugia malayi |
| 3.1.3.12 | TPP | - |
Brugia malayi |
| 3.1.3.12 | trehalose-6-phosphate phosphatase | - |
Brugia malayi |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.12 | 37 | - |
- |
Brugia malayi |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.12 | 25 | 60 | activity range | Brugia malayi |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.12 | 50 | - |
purified recombinant His-tagged enzyme, 10 min, stable up to | Brugia malayi |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.12 | 7 | - |
- |
Brugia malayi |
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