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Literature summary extracted from

  • Stano, J.; Siekel, P.; Neubert, K.; Micieta, K.
    Study of gherkin lactase in cell culture and in seedlings (2011), Acta Histochem., 113, 631-635.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 D-galactose 2% residual activity at 50 mM and 86% residual activity at 0.1 mM (purified lactase) Cucumis sativus
3.2.1.23 glucose 82% residual activity at 100 mM and 95% residual activity at 20 mM (purified lactase) Cucumis sativus
3.2.1.23 lactose 92% residual activity at 50 mM and 97% residual activity at 20 mM (purified lactase) Cucumis sativus
3.2.1.23 melibiose 44% residual activity at 100 mM and 86% residual activity at 0.1 mM (purified lactase); 44% residual activity at 100 mM and 86% residual activity at 10 mM (purified lactase) Cucumis sativus
3.2.1.23 additional information not inhibited by fructose, maltose and saccharose Cucumis sativus
3.2.1.23 raffinose 53% residual activity at 200 mM and 95% residual activity at 20 mM (purified lactase) Cucumis sativus
3.2.1.23 stachyose 90% residual activity at 100 mM and 79% residual activity at 10 mM (purified lactase) Cucumis sativus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Cucumis sativus
-
 cultivar Palava
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23 ammonium sulfate precipitation and Sephadex G-100 gel filtration Cucumis sativus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.23 seedling
-
 Cucumis sativus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.23 0.025
-
 crude homogenate, at pH 4.8, 60°C Cucumis sativus
3.2.1.23 0.27
-
 after 11.1fold purification, at pH 4.8, 60°C Cucumis sativus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 1-naphthyl beta-D-galactopyranoside + H2O
-
 Cucumis sativus 1-naphthol + beta-D-galactopyranose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O The enzyme hydrolysis of 2-nitrophenyl beta-D-galactopyranoside is linear within 3.5 h reaching 60-65% of substrate conversion, than practically stops Cucumis sativus 2-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.23 lactose + H2O
-
 Cucumis sativus D-galactose + D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.23 gherkin lactase
-
 Cucumis sativus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 56
-
 in permeabilized cell suspension Cucumis sativus
3.2.1.23 60
-
 in immobilized cell suspension Cucumis sativus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 4.8
-
-
 Cucumis sativus