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Literature summary extracted from

  • Goncalves, S.; Esteves, A.M.; Borges, N.; Santos, H.; Matias, P.M.
    Crystallization and preliminary X-ray analysis of mannosyl-3-phosphoglycerate phosphatase from Thermus thermophilus HB27 (2011), Acta Crystallogr. Sect. F, 67, 390-396.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.70 expression in Escherichia coli strain BL21 (DE3) Thermus thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.70 purified recombinant enzyme, sitting drop vapour diffusion technique, 10 mg/ml in 20 mM MES-NaOH, pH 6.35, 5 mM DTT, 1 mM EDTA, 5 mM MgCl2, and 760 mM NaCl, mixed with crystallization solution containing 0.02 M of each of the carboxylic acids, i.e. Na-formate, NH4-acetate, Na3-citrate, NaK-L-tartrate and Na-oxamate, 0.1 M MES/imidazole, pH 6.5, in a 1:1 molar ratio forming 0.002 ml drops, and a 30% v/v precipitant mixture containing 20% v/v ethylene glycol and 10% v/v PEG 8000, 20°C, 5 days, X-ray diffraction structure determination and analysis at 1.90 A resolution Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.70 28200
-
 2 * 28200, recombinant enzyme, SDS-PAGE Thermus thermophilus
3.1.3.70 56400
-
 recombinant enzyme, gel filtration Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.70 Thermus thermophilus Q72K29
-
-
3.1.3.70 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 Q72K29
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.70 recombinant enzyme from Escherichia coli strain BL21 (DE3) by anion exchange and cation exchange chromatography Thermus thermophilus

Subunits

EC Number Subunits Comment Organism
3.1.3.70 dimer 2 * 28200, recombinant enzyme, SDS-PAGE Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
3.1.3.70 More the enzyme is a member of the haloalkanoic acid dehalogenase superfamily Thermus thermophilus
3.1.3.70 MPGP
-
 Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.70 6.3
-
 assay at Thermus thermophilus

General Information

EC Number General Information Comment Organism
3.1.3.70 metabolism the synthesis of MG via mannosyl-3-phosphoglycerate synthase and mannosyl-3-phosphoglycerate phosphatase, the so-called two-step pathway, is the most prevalent route among these organisms. The phosphorylated intermediate mannosyl-3-phosphoglycerate is synthesized by the first enzyme and is subsequently dephosphorylated by the second Thermus thermophilus