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Literature summary extracted from

  • Panosian, T.D.; Nannemann, D.P.; Bachmann, B.O.; Iverson, T.M.
    Crystallization and preliminary X-ray analysis of a phosphopentomutase from Bacillus cereus (2010), Acta Crystallogr. Sect. F, 66, 811-814.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.2.7 expressed in Escherichia coli BL21-Gold (DE3) cells Bacillus cereus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.4.2.7 hanging drop vapor diffusion method, using 100 mM Bis-Tris pH 5.5, 50 mM MnCl2, 13-16% PEG 3350, and 25-100 mM NH4CH3COO Bacillus cereus
5.4.2.7 hanging drop vapor diffusion method, using 100 mM Bis-Tris pH 5.5, 50 mM MnCl2, 13-16% PEG 3350, and 25–-100 mM NH4CH3COO Bacillus cereus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.2.7 44000
-
 x * 44000, SDS-PAGE Bacillus cereus

Organism

EC Number Organism UniProt Comment Textmining
5.4.2.7 Bacillus cereus
-
-
-
5.4.2.7 Bacillus cereus ATCC 14579D
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.2.7 HisTrap HP column chromatography and Superdex 200 gel filtration Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.2.7 alpha-D-ribose 1-phosphate
-
 Bacillus cereus D-ribose 5-phosphate
-
 r
5.4.2.7 alpha-D-ribose 1-phosphate
-
 Bacillus cereus ATCC 14579D D-ribose 5-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
5.4.2.7 ? x * 44000, SDS-PAGE Bacillus cereus

Synonyms

EC Number Synonyms Comment Organism
5.4.2.7 PPM
-
 Bacillus cereus