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Literature summary extracted from

  • Petrareanu, G.; Balasu, M.C.; Zander, U.; Scheidig, A.J.; Szedlacsek, S.E.
    Preliminary X-ray crystallographic analysis of the D-xylulose 5-phosphate phosphoketolase from Lactococcus lactis (2010), Acta Crystallogr. Sect. F, 66, 805-807.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.9 expressed in Escherichia coli BL21 (DE3) RIL cells Lactococcus lactis
4.1.2.9 expressed in Escherichia coli BL21(DE3) RIL cells Lactococcus lactis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.2.9 hanging drop vapor diffusion method, using 17% (w/v) PEG 3350 and 0.2 M NaSCN, at 18°C Lactococcus lactis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.9 Mg2+ cofactor-bound Mg2+ is required for enzymatic activity Lactococcus lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.9 95727
-
 x * 95727, calculated from amino acid sequence Lactococcus lactis

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.9 Lactococcus lactis
-
-
-
4.1.2.9 Lactococcus lactis
-
 subsp. lactis
-
4.1.2.9 Lactococcus lactis IL 1403
-
-
-
4.1.2.9 Lactococcus lactis IL 1403
-
 subsp. lactis
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.9 HisTrap HP column chromatography and Superdex 200 gel filtration Lactococcus lactis
4.1.2.9 HisTrap Ni2+ metal affinity column chromatography and Superdex 200 gel filtration Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.9 D-Fructose 6-phosphate + phosphate the enzyme has higher activity with D-xylulose 5-phosphate than D-fructose 6-phosphate Lactococcus lactis Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
 ?
4.1.2.9 D-Fructose 6-phosphate + phosphate the specificity constant for D-xylulose 5-phosphate is significantly higher than that for D-fructose 6-phosphate Lactococcus lactis Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
 ?
4.1.2.9 D-Fructose 6-phosphate + phosphate the enzyme has higher activity with D-xylulose 5-phosphate than D-fructose 6-phosphate Lactococcus lactis IL 1403 Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
 ?
4.1.2.9 D-Fructose 6-phosphate + phosphate the specificity constant for D-xylulose 5-phosphate is significantly higher than that for D-fructose 6-phosphate Lactococcus lactis IL 1403 Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
 ?
4.1.2.9 D-Xylulose 5-phosphate + phosphate the specificity constant for D-xylulose 5-phosphate is significantly higher than that for D-fructose 6-phosphate Lactococcus lactis Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
 ?
4.1.2.9 D-Xylulose 5-phosphate + phosphate the specificity constant for D-xylulose 5-phosphate is significantly higher than that for D-fructose 6-phosphate Lactococcus lactis IL 1403 Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
 ?
4.1.2.9 D-xylulose 5-phosphate + phosphate most specific substrate Lactococcus lactis acetyl phosphate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.9 D-xylulose 5-phosphate + phosphate most specific substrate Lactococcus lactis IL 1403 acetyl phosphate + D-glyceraldehyde 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.9 ? x * 95727, calculated from amino acid sequence Lactococcus lactis

Synonyms

EC Number Synonyms Comment Organism
4.1.2.9 D-xylulose 5-phosphate phosphoketolase
-
 Lactococcus lactis

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.9 thiamine diphosphate
-
 Lactococcus lactis
4.1.2.9 thiamine diphosphate dependent on Lactococcus lactis