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Literature summary extracted from

  • Ito, T.; Kiyasu, N.; Matsunaga, R.; Takahashi, S.; Yokoyama, S.
    Structure of nondiscriminating glutamyl-tRNA synthetase from Thermotoga maritima (2010), Acta Crystallogr. Sect. D, 66, 813-820.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.17 TM1351, sequence comparisons, expression in Escherichia coli strain Rosetta2(DE3) Thermotoga maritima
6.1.1.24 expressed in Escherichia coli Thermotoga maritima
6.1.1.24 TM1875, sequence comparisons, expression in Escherichia coli strain Rosetta2(DE3) Thermotoga maritima

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.1.1.24 the crystal structure of the Thermotoga maritima ND-GluRS, TM1875, is determined in complex with a Glu-AMP analogue at 2.0 A resolution. ND-GluRS contains a characteristic structure in the connective-peptide domain, which is inserted into the catalytic Rossmann-fold domain Thermotoga maritima
6.1.1.24 TM1875 in complex with a Glu-AMP analogue, mixing of 0.0018 ml of 15 mg/ml protein in 20 mM Tris-HCl, pH 7.0, containing 5 mM MgCl2, 10 mM 2-mercaptoethanol, 50 mM NaCl, and 1 mM L-glutamylsulfamoyl adenosine, with 0.0018 ml reservoir solution containing 100 mM HEPES-NaOH, pH 7.5, 8% ethylene glycol and 15% PEG 8000, and 0.0004 ml of 30% D-sorbitol, equilibration against 0.5 ml reservoir solution, 20°C, 1 week, X-ray diffraction structure determination and analysis at 2.0 A resolution Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.24 Mg2+
-
 Thermotoga maritima
6.1.1.24 Mg2+ required Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.24 additional information Thermotoga maritima ND-GluRS TM1875 glutamylates both tRNAGlu and tRNAGln ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.17 Thermotoga maritima
-
-
-
6.1.1.17 Thermotoga maritima TM1351
-
-
-
6.1.1.24 Thermotoga maritima Q9X2I8
-
-
6.1.1.24 Thermotoga maritima Q9X2I8 TM1875
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.17 recombinant TM1351 from Escherichia coli strain Rosetta2 (DE3) by anion exchange chromatography and gel filtration Thermotoga maritima
6.1.1.24 recombinant TM1875 from Escherichia coli strain Rosetta2 (DE3) by anion exchange chromatography and gel filtration Thermotoga maritima
6.1.1.24 using ion-exchange column chromatography and gel-filtration Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.17 additional information D-GluRS glutamylates tRNAGlu only Thermotoga maritima ?
-
 ?
6.1.1.17 additional information D-GluRS glutamylates tRNAGlu only Thermotoga maritima TM1351 ?
-
 ?
6.1.1.24 ATP + Glu + tRNAGln the glutamylation ability of tRNAGln by ND-GluRS is measured in the presence of the bacterial Glu-tRNAGln amidotransferase GatCAB. Glutamylation efficiency is not affected even in the presence of excess GatCAB Thermotoga maritima AMP + diphosphate + L-glutamyl-tRNAGln
-
 ?
6.1.1.24 additional information ND-GluRS TM1875 glutamylates both tRNAGlu and tRNAGln Thermotoga maritima ?
-
 ?
6.1.1.24 additional information TM1875 glutamylates both the tRNAGlu and tRNAGln transcripts Thermotoga maritima ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.17 More structure analysis, overview Thermotoga maritima
6.1.1.24 More Thermotoga maritima ND-GluRS contains a characteristic structure in the connective-peptide domain, which is inserted into the catalytic Rossmann-fold domain, structure, overview Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
6.1.1.17 D-GluRS
-
 Thermotoga maritima
6.1.1.17 discriminating GluRS
-
 Thermotoga maritima
6.1.1.17 GluRS
-
 Thermotoga maritima
6.1.1.17 TM1351
-
 Thermotoga maritima
6.1.1.24 Glutamyl-tRNA synthetase
-
 Thermotoga maritima
6.1.1.24 ND-GluRS
-
 Thermotoga maritima
6.1.1.24 nondiscriminating GluRS
-
 Thermotoga maritima
6.1.1.24 nondiscriminating glutamyl-tRNA synthetase
-
 Thermotoga maritima
6.1.1.24 TM1875
-
 Thermotoga maritima
6.1.1.24 TM1875 only TM1875 functions as a nondiscriminating GluRS Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.24 50
-
 assay at Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.24 7.5
-
 assay at Thermotoga maritima

General Information

EC Number General Information Comment Organism
6.1.1.24 physiological function ND-GluRS produces the intermediate Glu-tRNAGln, which is converted to Gln-tRNAGln by Glu-tRNAGln amidotransferase. GluRS avoids competition with Glu-tRNAGln amidotransferase GatCAB and glutamylates tRNAGln Thermotoga maritima