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Literature summary extracted from

  • Yi, C.; Yang, C.G.; He, C.
    A non-heme iron-mediated chemical demethylation in DNA and RNA (2009), Acc. Chem. Res., 42, 519-529.
    View publication on PubMedView publication on EuropePMC

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.33 Fe2+ AlkB uses a non-heme mononuclear iron(II) Escherichia coli
1.14.11.33 Fe2+ AlkB uses a non-heme mononuclear iron(II) Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.33 Escherichia coli
-
-
-
1.14.11.33 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.33 DNA-1,6-ethenoadenine + O2
-
 Escherichia coli DNA-adenine + glyoxal
-
 ?
1.14.11.33 DNA-1,6-ethenoadenine + O2
-
 Homo sapiens DNA-adenine + glyoxal
-
 ?
1.14.11.33 DNA-1-methyladenine + 2-oxoglutarate + O2 best substrate for AlkB Escherichia coli DNA-adenine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 DNA-1-methyladenine + 2-oxoglutarate + O2 DNA-1-methyladenine is demethylated by isoforms ABH2 and ABH3 Homo sapiens DNA-adenine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 DNA-1-methylguanine + 2-oxoglutarate + O2 DNA-1-methylguanine is also repaired by AlkB, but less efficiently as DNA-1-methyladenine or DNA-3-methylcytosine Escherichia coli DNA-guanine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 DNA-1-methylguanine + 2-oxoglutarate + O2 DNA-1-methylguanine is also repaired by AlkB, but less efficiently as DNA-1-methyladenine or DNA-3-methylcytosine Homo sapiens DNA-guanine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 DNA-3-methylcytosine + 2-oxoglutarate + O2 best substrate for AlkB Escherichia coli DNA-cytosine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 DNA-3-methylcytosine + 2-oxoglutarate + O2 DNA-1-methylcytosine is demethylated by isoforms ABH2 and ABH3. Isoform ABH1 exclusively repairs DNA-3-methylcytosine in single-stranded DNA with low activity Homo sapiens DNA-cytosine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 DNA-3-methylthymine + 2-oxoglutarate + O2 DNA-3-methylthymine is also repaired by AlkB, but less efficiently as DNA-1-methyladenine or DNA-3-methylcytosine Escherichia coli DNA-thymine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 DNA-3-methylthymine + 2-oxoglutarate + O2 DNA-3-methylthymine is also repaired by AlkB, but less efficiently as DNA-1-methyladenine or DNA-3-methylcytosine. Isoform FTO repairs DNA-3-methylthymine Homo sapiens DNA-thymine + formaldehyde + succinate + CO2
-
 ?
1.14.11.33 additional information isoform ABH2 prefers double-stranded DNA while isoforms ABH3 and ABH1 work better on single-stranded substrates Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.33 ABH1 isoform Homo sapiens
1.14.11.33 ABH2 isoform Homo sapiens
1.14.11.33 ABH3 isoform Homo sapiens
1.14.11.33 AlkB
-
 Escherichia coli
1.14.11.33 FTO isoform Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.33 2-oxoglutarate
-
 Escherichia coli
1.14.11.33 2-oxoglutarate
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
1.14.11.33 physiological function AlkB corrects some of the unwanted methylations of DNA bases by a unique oxidative demethylation in which the methyl carbon is liberated as formaldehyde. The enzyme also repairs exocyclic DNA lesions Escherichia coli
1.14.11.33 physiological function AlkB corrects some of the unwanted methylations of DNA bases by a unique oxidative demethylation in which the methyl carbon is liberated as formaldehyde. The enzyme also repairs exocyclic DNA lesions Homo sapiens