EC Number | Application | Comment | Organism |
---|---|---|---|
5.6.1.2 | diagnostics | the straightforward dynein-ATPase assay can be used to obtain data of functional interest in clinical or experimental settings, to test the effect of new drugs that could inhibit dynein-ATPase actitivty and therefor sperm motility | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.6.1.2 | EDTA | in presence of 1 mM, 12% activity compared to assay without EDTA | Homo sapiens | |
5.6.1.2 | Li+ | 6 mM, 72% inhibition | Homo sapiens | |
5.6.1.2 | additional information | insensitive to 2 mM ouabain, 0.001 mM oligomycin, and 0.001 mM thapsgargin | Homo sapiens | |
5.6.1.2 | vanadate | inhibited by 0.01 mM, this inhibition is reversed in the presence of 1 mM norepinephrine, by reducing vanadate to oxovanadium | Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
5.6.1.2 | Mg2+ | dependent on | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.6.1.2 | Homo sapiens | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
5.6.1.2 | sperm | axenome-containg fractions, free of plasma membranes, dynein ATPase is the intracelluar motor for sperm motility, from healthy donors with either normozoospermia or asthenozoospermia, as indicated by a sperm motility lower than 50% (WHO grade a + b) | Homo sapiens | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
5.6.1.2 | additional information | - |
the dynein-ATPase activity is significantly lower for asthenozoospermic donors as compared to normozoospermic dononors | Homo sapiens |
5.6.1.2 | 0.026 | - |
containing 2 mM MgCl2, pH 8.0. 37°C | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.6.1.2 | dynein-ATPase | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.6.1.2 | 37 | - |
assay at | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.6.1.2 | 8 | - |
assay at | Homo sapiens |