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Literature summary extracted from

  • Vívenes, C.Y.; Peralta-Arias, R.D.; Camejo, M.I.; Guerrero, K.; Fernández, V.H.; Pinero, S.; Proverbio, T.; Proverbio, F.; Marín, R.
    Biochemical identification of dynein-ATPase activity in human sperm (2009), Z. Naturforsch. C, 64, 747-753.
    View publication on PubMed

Application

EC Number Application Comment Organism
5.6.1.2 diagnostics the straightforward dynein-ATPase assay can be used to obtain data of functional interest in clinical or experimental settings, to test the effect of new drugs that could inhibit dynein-ATPase actitivty and therefor sperm motility Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.6.1.2 EDTA in presence of 1 mM, 12% activity compared to assay without EDTA Homo sapiens
5.6.1.2 Li+ 6 mM, 72% inhibition Homo sapiens
5.6.1.2 additional information insensitive to 2 mM ouabain, 0.001 mM oligomycin, and 0.001 mM thapsgargin Homo sapiens
5.6.1.2 vanadate inhibited by 0.01 mM, this inhibition is reversed in the presence of 1 mM norepinephrine, by reducing vanadate to oxovanadium Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.6.1.2 Mg2+ dependent on Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
5.6.1.2 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.6.1.2 sperm axenome-containg fractions, free of plasma membranes, dynein ATPase is the intracelluar motor for sperm motility, from healthy donors with either normozoospermia or asthenozoospermia, as indicated by a sperm motility lower than 50% (WHO grade a + b) Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.6.1.2 additional information
-
the dynein-ATPase activity is significantly lower for asthenozoospermic donors as compared to normozoospermic dononors Homo sapiens
5.6.1.2 0.026
-
containing 2 mM MgCl2, pH 8.0. 37°C Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
5.6.1.2 dynein-ATPase
-
Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.6.1.2 37
-
assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6.1.2 8
-
assay at Homo sapiens