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Literature summary extracted from

  • Antypa, A.; Rebello, C.; Biernacka, A.; Krajewski, K.; Cassam, J.; Mitchell, S.C.; Steventon, G.B.
    Post-translational activation of human phenylalanine 4-monooxygenase from an endobiotic to a xenobiotic enzyme by reactive oxygen and reactive nitrogen species (2010), Xenobiotica, 40, 319-330.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.16.1 expressed in Escherichia coli as a recombinant protein Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.16.1 0.05
-
 L-phenylalanine post-translational activation of human cDNA-expressed PAH, activator: NO (nitric oxide) (0.01 mM), Vmax: 33.05 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
1.14.16.1 0.06
-
 L-phenylalanine post-translational activation of human cDNA-expressed PAH, activator: ONOO- (peroxynitrite)(1 mM), Vmax: 32.65 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
1.14.16.1 0.07
-
 L-phenylalanine post-translational activation of human cDNA-expressed PAH, activator: HO (hydroxyl radical) (0.01 mM), Vmax: 32.3 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
1.14.16.1 0.12
-
 L-phenylalanine post-translational activation of human cDNA-expressed PAH, activator: lysophosphatidylcholine (5 mM), Vmax: 3.72 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
1.14.16.1 0.12
-
 L-phenylalanine post-translational activation of human cDNA-expressed PAH, activator: N-ethylmaleimide (50 mM), Vmax: 9.3 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
1.14.16.1 0.14
-
 L-phenylalanine post-translational activation of human cDNA-expressed PAH, activator: H2O2 (0.1 mM), Vmax: 28.4 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
1.14.16.1 0.14
-
 L-phenylalanine post-translational activation of human cDNA-expressed PAH, activator: L-Phe (4 mM), Vmax: 1.86 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
1.14.16.1 0.87
-
 L-phenylalanine post-translational activation of PAH in human hepatic cytosol fractions, activator: NO (nitric oxide) (0.01 mM), Vmax: 274.5 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
1.14.16.1 1.22
-
 L-phenylalanine post-translational activation of PAH in human hepatic cytosol fractions, activator: N-ethylmaleimide (50 mM), Vmax: 224 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
1.14.16.1 1.25
-
 L-phenylalanine post-translational activation of PAH in human hepatic cytosol fractions, activator: ONOO- (peroxynitrite)(1 mM), Vmax: 260.4 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
1.14.16.1 1.5 2 L-phenylalanine post-translational activation of PAH in human hepatic cytosol fractions, activator: HO (hydroxyl radical) (0.01 mM), Vmax: 241.6 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
1.14.16.1 2.38
-
 S-carboxymethyl-L-cysteine post-translational activation of human cDNA-expressed PAH, activator: NO (nitric oxide) (0.01 mM), Vmax: 42.32 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
1.14.16.1 2.75
-
 S-carboxymethyl-L-cysteine post-translational activation of human cDNA-expressed PAH, activator: H2O2 (0.1 mM), Vmax: 36.5 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
1.14.16.1 2.85
-
 L-phenylalanine post-translational activation of PAH in human hepatic cytosol fractions, activator: lysophosphatidylcholine (5 mM), Vmax: 29.1 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
1.14.16.1 3.01
-
 L-phenylalanine post-translational activation of PAH in human hepatic cytosol fractions, activator: H2O2 (0.1 mM), Vmax: 205 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
1.14.16.1 3.05
-
 L-phenylalanine post-translational activation of PAH in human hepatic cytosol fractions, activator: L-Phe (4 mM), Vmax: 14.23 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
1.14.16.1 3.2
-
 S-carboxymethyl-L-cysteine post-translational activation of human cDNA-expressed PAH, activator: HO (hydroxyl radical) (0.01 mM), Vmax: 36.5 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
1.14.16.1 4.1
-
 S-carboxymethyl-L-cysteine post-translational activation of human cDNA-expressed PAH, activator: ONOO- (peroxynitrite)(1 mM), Vmax: 41.27 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
1.14.16.1 4.38
-
 S-carboxymethyl-L-cysteine post-translational activation of PAH in human hepatic cytosol fractions, activator: NO (nitric oxide) (0.01 mM), Vmax: 500 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
1.14.16.1 4.62
-
 S-carboxymethyl-L-cysteine post-translational activation of PAH in human hepatic cytosol fractions, activator: H2O2 (0.1 mM), Vmax: 430 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
1.14.16.1 5.23
-
 S-carboxymethyl-L-cysteine post-translational activation of PAH in human hepatic cytosol fractions, activator: N-ethylmaleimide (50 mM), Vmax: 450 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
1.14.16.1 6.49
-
 S-carboxymethyl-L-cysteine post-translational activation of PAH in human hepatic cytosol fractions, activator: HO (hydroxyl radical) (0.01 mM), Vmax: 455 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
1.14.16.1 7.54
-
 S-carboxymethyl-L-cysteine post-translational activation of PAH in human hepatic cytosol fractions, activator: ONOO- (peroxynitrite)(1 mM), Vmax: 481 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
1.14.16.1 7.65
-
 S-carboxymethyl-L-cysteine post-translational activation of human cDNA-expressed PAH, activator: N-ethylmaleimide (50 mM), Vmax: 5.58 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
1.14.16.1 8.1
-
 S-carboxymethyl-L-cysteine post-translational activation of human cDNA-expressed PAH, activator: L-Phe (4 mM), Vmax: 0.07 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
1.14.16.1 8.3
-
 S-carboxymethyl-L-cysteine post-translational activation of human cDNA-expressed PAH, activator: lysophosphatidylcholine (5 mM), Vmax: 4.38 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
1.14.16.1 16.53
-
 S-carboxymethyl-L-cysteine post-translational activation of PAH in human hepatic cytosol fractions, activator: L-Phe (4 mM), Vmax: 0.87 nmoles/min/mg, pH 6.8, 37°C Homo sapiens
1.14.16.1 16.53
-
 S-carboxymethyl-L-cysteine post-translational activation of PAH in human hepatic cytosol fractions, activator: lysophosphatidylcholine (5 mM), Vmax: 52.31 nmoles/min/mg, pH 6.8, 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.1 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.16.1 hepatocyte enzyme isolated from human female hepatic cytosolic fractions Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.1 L-phenylalanine + tetrahydrobiopterin + O2
-
 Homo sapiens L-tyrosine + dihydrobiopterin + H2O
-
 ?
1.14.16.1 S-carboxymethyl-L-cysteine + tetrahydrobiopterin + O2
-
 Homo sapiens S-carboxymethyl-L-cysteine S-oxide + dihydrobiopterin + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.16.1 PAH
-
 Homo sapiens
1.14.16.1 phenylalanine hydroxylase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.16.1 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.16.1 6.8
-
 assay at Homo sapiens