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Literature summary extracted from

  • Santoro, M.L.; Vaquero, T.S.; Leme, A.F.; Serrano, S.M.
    NPP-BJ, a nucleotide pyrophosphatase/phosphodiesterase from Bothrops jararaca snake venom, inhibits platelet aggregation (2009), Toxicon, 54, 499-512.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.9 dithiothreitol less than 20% residual activity at 40 mM dithiothreitol using ATP as substrate, less than 10% residual activity at 40 mM dithiothreitol using ADP as substrate, less than 50% residual activity at 40 mM dithiothreitol using bis(4-nitrophenyl) phosphate as substrate Bothrops jararaca
3.6.1.9 EDTA less than 20% residual activity at 40 mM EDTA using ATP as substrate, less than 10% residual activity at 40 mM EDTA using ADP as substrate, about 40% residual activity at 40 mM EDTA using bis(4-nitrophenyl) phosphate as substrate Bothrops jararaca
3.6.1.9 additional information phenylmethylsulfonyl fluoride does not modify the catalytic activity of NPP Bothrops jararaca
3.6.1.9 Sodium fluoride about 30% residual activity at 40 mM sodium fluoride using ATP as substrate, less than 60% residual activity at 40 mM sodium fluoride using ADP or bis(4-nitrophenyl) phosphate as substrate Bothrops jararaca

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.9 0.85
-
 p-nitrophenyl 5'-thymidine monophosphate in 0.1 M glycine-NaOH, pH 8.9, at 37°C Bothrops jararaca
3.6.1.9 21.88
-
 bis(4-nitrophenyl) phosphate in 0.1 M glycine-NaOH, pH 8.9, at 37°C Bothrops jararaca

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.9 288000
-
 gel filtration Bothrops jararaca

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.9 Bothrops jararaca
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.9 blue-Sepharose column chromatography, oligo-dT cellulose column chromatography, and Sephacryl S-300 gel filtration Bothrops jararaca

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.9 venom
-
 Bothrops jararaca
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.9 0.16
-
 crude enzyme, using ADP as substrate, in 0.1 M glycine-NaOH, pH 8.9, at 37°C Bothrops jararaca
3.6.1.9 0.17
-
 crude enzyme, using ATP as substrate, in 0.1 M glycine-NaOH, pH 8.9, at 37°C Bothrops jararaca
3.6.1.9 0.67
-
 crude enzyme, using bis(4-nitrophenyl) phosphate as substrate, in 0.1 M glycine-NaOH, pH 8.9, at 37°C Bothrops jararaca
3.6.1.9 1.3
-
 after 8.3fold purification, using ADP as substrate, in 0.1 M glycine-NaOH, pH 8.9, at 37°C Bothrops jararaca
3.6.1.9 10.3
-
 after 61.6fold purification, using ATP as substrate, in 0.1 M glycine-NaOH, pH 8.9, at 37°C Bothrops jararaca
3.6.1.9 242
-
 after 362.5fold purification, using bis(4-nitrophenyl) phosphate as substrate, in 0.1 M glycine-NaOH, pH 8.9, at 37°C Bothrops jararaca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.9 ADP + H2O 5fold lower activity compared to ATP Bothrops jararaca AMP + phosphate
-
 ?
3.6.1.9 ATP + H2O the specific activity of NPP for ATP is about 5fold higher than that of ADP Bothrops jararaca ADP + phosphate
-
 ?
3.6.1.9 ATP + H2O
-
 Bothrops jararaca AMP + diphosphate
-
 ?
3.6.1.9 bis(4-nitrophenyl) phosphate + H2O
-
 Bothrops jararaca 4-nitrophenylphosphate + 4-nitrophenol
-
 ?
3.6.1.9 CDP + H2O
-
 Bothrops jararaca CMP + phosphate
-
 ?
3.6.1.9 CTP + H2O
-
 Bothrops jararaca CDP + phosphate
-
 ?
3.6.1.9 dATP + H2O
-
 Bothrops jararaca dADP + phosphate
-
 ?
3.6.1.9 dCDP + H2O
-
 Bothrops jararaca dCMP + phosphate
-
 ?
3.6.1.9 dCTP + H2O
-
 Bothrops jararaca dCDP + phosphate
-
 ?
3.6.1.9 dGDP + H2O
-
 Bothrops jararaca dGMP + phosphate
-
 ?
3.6.1.9 dGTP + H2O
-
 Bothrops jararaca dGDP + phosphate
-
 ?
3.6.1.9 GDP + H2O
-
 Bothrops jararaca GMP + phosphate
-
 ?
3.6.1.9 GTP + H2O
-
 Bothrops jararaca GDP + phosphate
-
 ?
3.6.1.9 IDP + H2O
-
 Bothrops jararaca IMP + phosphate
-
 ?
3.6.1.9 ITP + H2O
-
 Bothrops jararaca IDP + phosphate
-
 ?
3.6.1.9 additional information the enzyme is not active upon AMP, GMP, dAMP, IMP, CMP, dGMP, dADP, TDP, TMP, UMP, dCMP, 3'-AMP, cAMP, and 4-nitrophenyl phosphate Bothrops jararaca ?
-
 ?
3.6.1.9 additional information the enzyme preferentially hydrolyzes nucleoside 5'-triphosphates over nucleoside 5'-diphosphates Bothrops jararaca ?
-
 ?
3.6.1.9 p-nitrophenyl 5'-thymidine monophosphate + H2O
-
 Bothrops jararaca 4-nitrophenol + TMP
-
 ?
3.6.1.9 TTP + H2O
-
 Bothrops jararaca TDP + phosphate
-
 ?
3.6.1.9 UDP + H2O
-
 Bothrops jararaca UMP + phosphate
-
 ?
3.6.1.9 UTP + H2O highest rate of hydrolysis Bothrops jararaca UDP + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.9 NPP
-
 Bothrops jararaca
3.6.1.9 nucleotide pyrophosphatase/phosphodiesterase
-
 Bothrops jararaca

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.9 44000
-
 bis(4-nitrophenyl) phosphate in 0.1 M glycine.NaOH, pH 8.9, at 37°C Bothrops jararaca
3.6.1.9 960000
-
 p-nitrophenyl 5'-thymidine monophosphate in 0.1 M glycine.NaOH, pH 8.9, at 37°C Bothrops jararaca

General Information

EC Number General Information Comment Organism
3.6.1.9 physiological function the enzyme inhibits ADP-induced platelet aggregation, whereas thrombin-induced platelet aggregation is only slightly attenuated Bothrops jararaca

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.6.1.9 2000
-
 bis(4-nitrophenyl) phosphate in 0.1 M glycine-NaOH, pH 8.9, at 37°C Bothrops jararaca
3.6.1.9 11000000
-
 S-5'-deoxyadenosylglutathione in 0.1 M glycine-NaOH, pH 8.9, at 37°C Bothrops jararaca