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Literature summary extracted from

  • Abdel-Naim, A.B.; Mohamadin, A.M.
    Myeloperoxidase-catalyzed oxidation of chloroacetonitrile to cyanide (2004), Toxicol. Lett., 146, 249-257.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.11.2.2 D-penicillamine addition of D-penicillamine significantly enhances the rate of chloroacetonitrile oxidation and cyanide release by the myeloperoxidase/H2O2/Cl- system (17.2% increase at 5 mM) Homo sapiens
1.11.2.2 glutathione addition of glutathione significantly enhances the rate of chloroacetonitrile oxidation and cyanide release by the myeloperoxidase/H2O2/Cl- system (24% increase at 5 mM) Homo sapiens
1.11.2.2 L-cysteine addition of L-cysteine significantly enhances the rate of chloroacetonitrile oxidation and cyanide release by the myeloperoxidase/H2O2/Cl- system (45.4% increase at 5 mM) Homo sapiens
1.11.2.2 N-acetyl-L-cysteine addition of N-acetyl-L-cysteine significantly enhances the rate of chloroacetonitrile oxidation and cyanide release by the myeloperoxidase/H2O2/Cl- system (16.3% increase at 5 mM) Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.2.2 4-aminobenzoic acid hydrazine metabolism of chloroacetonitrile to cyanide by MPO/H2O2/Cl- system is significantly reduced by 4-amino benzoic acid hydrazine to 52.6% of control value Homo sapiens
1.11.2.2 catalase addition of the antioxidant enzyme catalase to incubation mixtures results in 56.1% decrease in cyanide release by MPO/H2O2/Cl- system Homo sapiens
1.11.2.2 indomethacin metabolism of chloroacetonitrile to cyanide by MPO/H2O2/Cl- system is significantly reduced by indomethacin to 88.9% of control value Homo sapiens
1.11.2.2 Sodium azide metabolism of chloroacetonitrile to cyanide by MPO/H2O2/Cl- system is significantly reduced by sodium azide to 43.9% of control value Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.2.2 0.089
-
 Chloroacetonitrile at pH 5.5, 37°C, 0.1 mM KCl, 0.5 mM H2O2, 0.15 mM chloroacetonitrile, 5 units/ml MPO, and 0.25 mM NaOCl during an incubation period of 60 min Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.11.2.2 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.2.2 leukocyte
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.2.2 Cl- + H2O2 + chloroacetonitrile chloride ion (Cl-) is the dominant electron donor, chloroacetonitrile is activated to cyanide by myeloperoxidase/H2O2/Cl- system in vitro Homo sapiens HClO + H2O + cyanide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.2.2 MPO
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.2.2 37
-
 optimum temperature for the activation of chloroacetonitrile to cyanide by the myeloperoxidase/H2O2/Cl- system Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.2.2 5.5
-
 optimum pH for the activation of chloroacetonitrile to cyanide by the myeloperoxidase/H2O2/Cl- system Homo sapiens