Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ramu, R.; Chang, C.; Chou, H.; Wu, L.; Chiang, C.; Yu, S.
    Regio-selective hydroxylation of gem-difluorinated octanes by alkane hydroxylase (AlkB) (2011), Tetrahedron Lett., 52, 2950-2953.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.14.15.3 additional information the enzyme is useful in a whole-cell biotransformation system by recombinant expression in Escherichia coli to regioselectively synthesize 2,2-, 3,3- and 4,4-difluorooctan-1-ols, from simple and inexpensive starting materials, method evaluation, overview Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.14.15.3 additional information development of a whole-cell biotransformation system expressing AlkB in Escherichia coli to regioselectively synthesize 2,2-, 3,3- and 4,4-difluorooctan-1-ols, from simple and inexpensive starting materials, method evaluation, overview Pseudomonas putida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.15.3 membrane integral membrane-bound protein Pseudomonas putida 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.15.3 Fe2+ a nonheme diiron monooxygenase Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.3 Pseudomonas putida
-
-
-
1.14.15.3 Pseudomonas putida GPo1 / ATCC29347
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.3 additional information AlkB, a nonheme diiron monooxygenase, performs regioselectibe hydroxylation of gem-difluorinated octanes, synthesis of 2,2-, 3,3- and 4,4-difluorooctan-1-ols from 1-octanal and 2-, 3-, 4-octanones, NMR and GC/MS product analysis, overview. Reactions of AlkB with 3,3- and 4,4-difluorooctanes and 1,1,1-trifluoroctane, synthesis of 2,2-, 3,3- 4,4-difluorooctan-1-ols, and of 8,8-, 7,7-, 6,6- and 5,5-difluorooctan-1-ols, overview Pseudomonas putida ?
-
 ?
1.14.15.3 additional information AlkB, a nonheme diiron monooxygenase, performs regioselectibe hydroxylation of gem-difluorinated octanes, synthesis of 2,2-, 3,3- and 4,4-difluorooctan-1-ols from 1-octanal and 2-, 3-, 4-octanones, NMR and GC/MS product analysis, overview. Reactions of AlkB with 3,3- and 4,4-difluorooctanes and 1,1,1-trifluoroctane, synthesis of 2,2-, 3,3- 4,4-difluorooctan-1-ols, and of 8,8-, 7,7-, 6,6- and 5,5-difluorooctan-1-ols, overview Pseudomonas putida GPo1 / ATCC29347 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.15.3 alkane hydroxylase
-
 Pseudomonas putida
1.14.15.3 AlkB
-
 Pseudomonas putida
1.14.15.3 diiron omega-hydroxylase
-
 Pseudomonas putida

General Information

EC Number General Information Comment Organism
1.14.15.3 additional information AlkB contains a nonheme diiron center as a catalytic site Pseudomonas putida