Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Schirmer, A.; Rude, M.A.; Li, X.; Popova, E.; del Cardayre, S.B.
    Microbial biosynthesis of alkanes (2010), Science, 329, 559-562.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.80 expression of strain PCC7942 orf1593 and orf1594, the latter encoding an aldehde decarbonylase, both separately and together in Escherichia coli. Extracts of recombinant PCC7942_orf1594-expressing cells contain substantial quantities of evenchain fatty aldehydes and fatty alcohols, whereas coexpression of both PCC7942_orf1593 and orf1594 results in the production of odd chain alkanes and alkenes, as well as even-chain fatty aldehydes and fatty alcohols. Orf1593 and orf1594 appear to be part of a larger operon that contains a gene encoding a subunit of the acetyl-CoA carboxylase, accA, which is essential for growth Synechococcus sp.
4.1.99.5 expressed in Escherichia coli Nostoc punctiforme

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.80 additional information replacement of the PC7942_orf1593 and 1594 orthologues in Synechocystis sp. PCC6803, where the orthologous genes apparently form only a bicistronic operon, with a kanamycin-resistance cassette. This replacement abolishes the presence of alkanes in the extracts of photoautoptrophically grown cells. PCC7942_orf 1593 and orf1594 coexpression in an Escherichia coli strain devoid of acyl-CoAs leads to very similar levels of hydrocarbon production, comparable to those in an Escherichia coli wild-type strain Synechococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.80 0.0079
-
 oleoyl-[acyl carrier protein] pH and temperature not specified in the publication Synechococcus elongatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.80 fatty acyl-[acyl carrier protein] + NAD(P)H + H+ Synechococcus elongatus kinetically preferred and likely the in vivo substrate fatty aldehyde + acyl carrier protein + NAD(P)+
-
 ?
1.2.1.80 fatty acyl-[acyl carrier protein] + NAD(P)H + H+ Synechococcus elongatus PCC 7942 kinetically preferred and likely the in vivo substrate fatty aldehyde + acyl carrier protein + NAD(P)+
-
 ?
1.2.1.80 hexadecanoyl-[acyl-carrier protein] + NAD(P)H + H+ Synechococcus sp. acyl-ACP is kinetically preferred and is likely the in vivo substrate hexadecanal + acyl-carrier protein + NAD(P)+
-
 ?
1.2.1.80 hexadecanoyl-[acyl-carrier protein] + NAD(P)H + H+ Synechococcus sp. PCC 7942 acyl-ACP is kinetically preferred and is likely the in vivo substrate hexadecanal + acyl-carrier protein + NAD(P)+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.80 Synechococcus elongatus
-
-
-
1.2.1.80 Synechococcus elongatus PCC 7942
-
-
-
1.2.1.80 Synechococcus sp.
-
 orf1594 or sll0209
-
1.2.1.80 Synechococcus sp. PCC 7942
-
 orf1594 or sll0209
-
4.1.99.5 Nostoc punctiforme
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.99.5
-
 Nostoc punctiforme

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.80 fatty acyl-[acyl carrier protein] + NAD(P)H + H+ kinetically preferred and likely the in vivo substrate Synechococcus elongatus fatty aldehyde + acyl carrier protein + NAD(P)+
-
 ?
1.2.1.80 fatty acyl-[acyl carrier protein] + NAD(P)H + H+ kinetically preferred and likely the in vivo substrate Synechococcus elongatus PCC 7942 fatty aldehyde + acyl carrier protein + NAD(P)+
-
 ?
1.2.1.80 hexadecanoyl-[acyl-carrier protein] + NAD(P)H + H+ substrate selectivity Synechococcus sp. hexadecanal + acyl-carrier protein + NAD(P)+
-
 ?
1.2.1.80 hexadecanoyl-[acyl-carrier protein] + NAD(P)H + H+ acyl-ACP is kinetically preferred and is likely the in vivo substrate Synechococcus sp. hexadecanal + acyl-carrier protein + NAD(P)+
-
 ?
1.2.1.80 hexadecanoyl-[acyl-carrier protein] + NAD(P)H + H+ substrate selectivity Synechococcus sp. PCC 7942 hexadecanal + acyl-carrier protein + NAD(P)+
-
 ?
1.2.1.80 hexadecanoyl-[acyl-carrier protein] + NAD(P)H + H+ acyl-ACP is kinetically preferred and is likely the in vivo substrate Synechococcus sp. PCC 7942 hexadecanal + acyl-carrier protein + NAD(P)+
-
 ?
1.2.1.80 additional information does not reduce acyl-CoA Synechococcus elongatus ?
-
 ?
1.2.1.80 additional information does not reduce acyl-CoA Synechococcus elongatus PCC 7942 ?
-
 ?
1.2.1.80 oleoyl-[acyl carrier protein] + NAD(P)H + H+
-
 Synechococcus elongatus oleic aldehyde + acyl carrier protein + NAD(P)+
-
 ?
1.2.1.80 oleoyl-[acyl carrier protein] + NAD(P)H + H+
-
 Synechococcus elongatus PCC 7942 oleic aldehyde + acyl carrier protein + NAD(P)+
-
 ?
4.1.99.5 octadecanal + NADPH + O2 only observed in the presence of ferredoxin, ferredoxin reductase and NADPH Nostoc punctiforme heptadecane + formate + H2O + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.80 AAR
-
 Synechococcus sp.
1.2.1.80 AAR
-
 Synechococcus elongatus
1.2.1.80 acyl-ACP reductase
-
 Synechococcus sp.
1.2.1.80 acyl-ACP reductase
-
 Synechococcus elongatus
1.2.1.80 acyl-acyl carrier protein reductase
-
 Synechococcus elongatus
1.2.1.80 fatty aldehyde-generating acyl-ACP reductase
-
 Synechococcus sp.
4.1.99.5 aldehyde decarbonylase
-
 Nostoc punctiforme

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.80 NAD(P)H
-
 Synechococcus sp.
1.2.1.80 NAD(P)H
-
 Synechococcus elongatus

General Information

EC Number General Information Comment Organism
4.1.99.5 physiological function alkane biosynthesis pathway Nostoc punctiforme