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Literature summary extracted from

  • Xing, F.; Martzen, M.R.; Phizicky, E.M.
    A conserved family of Saccharomyces cerevisiae synthases effects dihydrouridine modification of tRNA (2002), RNA, 8, 370-381.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.88 expression in Escherichia coli Saccharomyces cerevisiae
1.3.1.91 expression in Escherichia coli Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.88 uracil17 in tRNA + NAD(P)H + H+ Saccharomyces cerevisiae
-
 5,6-dihydrouracil17 in tRNA + NAD(P)+
-
 ?
1.3.1.91 uracil in tRNA + NAD(P)H + H+ Saccharomyces cerevisiae
-
 5,6-dihydrouracil in tRNA + NAD(P)+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.88 Saccharomyces cerevisiae P53759
-
-
1.3.1.91 Saccharomyces cerevisiae P53720
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.88 recombinant enzyme Saccharomyces cerevisiae
1.3.1.91 recombinant enzyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.88 uracil17 in pre-tRNAPhe + NAD(P)H + H+
-
 Saccharomyces cerevisiae 5,6-dihydrouracil17 in pre-tRNAPhe + NAD(P)+
-
 ?
1.3.1.88 uracil17 in tRNA + NAD(P)H + H+
-
 Saccharomyces cerevisiae 5,6-dihydrouracil17 in tRNA + NAD(P)+
-
 ?
1.3.1.91 uracil in pre-tRNALeu + NAD(P)H + H+
-
 Saccharomyces cerevisiae 5,6-dihydrouracil in pre-tRNALeu + NAD(P)+
-
 ?
1.3.1.91 uracil in pre-tRNATyr + NAD(P)H + H+
-
 Saccharomyces cerevisiae 5,6-dihydrouracil in pre-tRNATyr + NAD(P)+
-
 ?
1.3.1.91 uracil in tRNA + NAD(P)H + H+
-
 Saccharomyces cerevisiae 5,6-dihydrouracil in tRNA + NAD(P)+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.88 Dus1p
-
 Saccharomyces cerevisiae
1.3.1.88 tRNA-dihydrouridine synthase 1
-
 Saccharomyces cerevisiae
1.3.1.91 Dus2p
-
 Saccharomyces cerevisiae
1.3.1.91 SMM1
-
 Saccharomyces cerevisiae
1.3.1.91 tRNA-dihydrouridine synthase 2
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.88 30
-
 assay at Saccharomyces cerevisiae
1.3.1.91 30
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.88 8
-
 assay at Saccharomyces cerevisiae
1.3.1.91 8
-
 assay at Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.88 FAD stimulates activity 25fold Saccharomyces cerevisiae
1.3.1.91 FAD stimulates activity Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
1.3.1.88 malfunction yeast extract from a dus1-DELTA strain is completely defective in modification of yeast pre-tRNAPhe Saccharomyces cerevisiae
1.3.1.91 malfunction yeast extract from a dus1-DELTA strain is completely defective in modification of yeast pre-tRNAPhe Saccharomyces cerevisiae