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Literature summary extracted from

  • Caldinelli, L.; Molla, G.; Bracci, L.; Lelli, B.; Pileri, S.; Cappelletti, P.; Sacchi, S.; Pollegioni, L.
    Effect of ligand binding on human D-amino acid oxidase: implications for the development of new drugs for schizophrenia treatment (2010), Protein Sci., 19, 1500-1512.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.3 expression of EGFP-tagged DAAO in U-87 glioblastoma cells, transient coexpression of pLG72 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.3 benzoate a classical substrate-competitive inhibitor, effect of benzoate on the FAD-binding process to hDAAO apoprotein Homo sapiens
1.4.3.3 chlorpromazine competitive versus cofactor FAD Homo sapiens
1.4.3.3 additional information benzoate and CPZ similarly modify the short-term cellular D-serine concentration but affect the cellular concentration of hDAAO differently Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.3 additional information
-
 additional information DAAO-lingand interaction kinetics, overview Homo sapiens
1.4.3.3 4
-
 D-serine pH 8.5, 25°C, recombinant EGFP-DAAO Homo sapiens
1.4.3.3 7
-
 D-serine pH 8.5, 25°C, recombinant wild-type DAAO Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.3 D-serine + H2O + O2 Homo sapiens the apoprotein form of hDAAO binds the substrate D-serine, which increases FAD binding thus increasing the amount of active holoenzyme in solution 2-oxo-3-hydroxypropionate + NH3 + H2O2
-
 ?
1.4.3.3 additional information Homo sapiens DAAO interacts with its physiological partner pLG72, the interaction is not inhibited by benzoate and chlorpromazine ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.3 Homo sapiens P14920
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.3 brain
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.3 D-serine + H2O + O2
-
 Homo sapiens 2-oxo-3-hydroxypropionate + NH3 + H2O2
-
 ?
1.4.3.3 D-serine + H2O + O2 the apoprotein form of hDAAO binds the substrate D-serine, which increases FAD binding thus increasing the amount of active holoenzyme in solution Homo sapiens 2-oxo-3-hydroxypropionate + NH3 + H2O2
-
 ?
1.4.3.3 additional information DAAO interacts with its physiological partner pLG72, the interaction is not inhibited by benzoate and chlorpromazine Homo sapiens ?
-
 ?
1.4.3.3 additional information comparison of the kinetics of trypsin cleavage of hDAAO in the presence of various ligands, overview Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.3.3 DAAO
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.3 25
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.3 2.5
-
 D-serine pH 8.5, 25°C, recombinant EGFP-DAAO Homo sapiens
1.4.3.3 3
-
 D-serine pH 8.5, 25°C, recombinant wild-type DAAO Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.3 8.5
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.3 FAD the apoprotein form of hDAAO binds the substrate D-serine, this interaction increases FAD binding thus increasing the amount of active holoenzyme in solution, binding analysis of FAD, overview Homo sapiens

General Information

EC Number General Information Comment Organism
1.4.3.3 physiological function in human brain the flavoprotein D-amino acid oxidase is responsible for the degradation of the neuromodulator D-serine, an important effector of NMDA-receptor mediated neurotransmission Homo sapiens