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Literature summary extracted from

  • Can, B.; Kulaksiz Erkmen, G.; Dalmizrak, O.; Ogus, I.H.; Ozer, N.
    Purification and characterisation of rat kidney glutathione reductase (2010), Protein J., 29, 250-256.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.7 0.0153
-
 NADPH in 100 mM potassium phosphate buffer, pH 7.4, 4 mM EDTA, at 37°C Rattus norvegicus
1.8.1.7 0.0531
-
 glutathione disulfide in 100 mM potassium phosphate buffer, pH 7.4, 4 mM EDTA, at 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.1.7 cytosol
-
 Rattus norvegicus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.7 53000
-
 native PAGE and SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.7 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.7 heat-denaturation and Sephadex G25 gel filtration, 2',5'-ADP agarose 4B column chromatography, and PBE94 column chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.7 kidney
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.7 0.11
-
 crude liver homogenate, in 100 mM potassium phosphate buffer, pH 7.4, 4 mM EDTA, at 37°C Rattus norvegicus
1.8.1.7 250
-
 after 2356fold purification, in 100 mM potassium phosphate buffer, pH 7.4, 4 mM EDTA, at 37°C Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.7 glutathione disulfide + NADH + H+ NADH is used as a coenzyme with a lower efficiency (32.7%) than NADPH Rattus norvegicus glutathione + NAD+
-
 ?
1.8.1.7 glutathione disulfide + NADPH + H+
-
 Rattus norvegicus glutathione + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.7 glutathione reductase
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.1.7 65
-
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.7 6.5
-
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.7 FAD each mol of enzyme subunit binds 0.97 mol of FAD Rattus norvegicus
1.8.1.7 NADH NADH is used as a coenzyme by rat kidney glutathione reductase but with a lower efficiency (32.7%) than NADPH Rattus norvegicus
1.8.1.7 NADPH
-
 Rattus norvegicus

General Information

EC Number General Information Comment Organism
1.8.1.7 physiological function glutathione reductase is the crucial enzyme to maintain high glutathione/glutathione disulfide ratio and physiological redox status in cells Rattus norvegicus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8.1.7 4850
-
 glutathione disulfide in 100 mM potassium phosphate buffer, pH 7.4, 4 mM EDTA, at 37°C Rattus norvegicus
1.8.1.7 16800
-
 NADPH in 100 mM potassium phosphate buffer, pH 7.4, 4 mM EDTA, at 37°C Rattus norvegicus