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Literature summary extracted from
- Zebrafish 10-formyltetrahydrofolate dehydrogenase is similar to its mammalian isozymes for its structural and catalytic properties (2010), Protein Expr. Purif., 72, 217-222.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.5.1.6 | expression in Escherichia coli and Pichia pastoris | Danio rerio |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.5.1.6 | 0.0127 | - |
10-formyltetrahydrofolate | 30°C, pH not specified in the publication, recombinant N-terminal domain | Danio rerio |  |
| 1.5.1.6 | 0.0156 | - |
10-formyltetrahydrofolate | 30°C, pH not specified in the publication, full-length recombinant enzyme | Danio rerio | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.5.1.6 | 100000 | - |
4 * 100000, calculated | Danio rerio |
| 1.5.1.6 | 400000 | - |
gel fitlration | Danio rerio |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.5.1.6 | Danio rerio | E3NZ06 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.5.1.6 | recombinant protein | Danio rerio |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.5.1.6 | 1.04 | - |
30°C, pH not specified in the publication, recombinant enzyme expressed in Pichia pastoris | Danio rerio |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.1.6 | 10-formyltetrahydrofolate + NADP+ + H2O | - |
Danio rerio | tetrahydrofolate + CO2 + NADPH + H+ | - |
? | |
| 1.5.1.6 | additional information | enzyme does not catalyze the oxidation of 10-formyltetrahydrofolate or propanal | Danio rerio | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.5.1.6 | tetramer | 4 * 100000, calculated | Danio rerio |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.5.1.6 | 97 | - |
10-formyltetrahydrofolate | 30°C, pH not specified in the publication, full-length recombinant enzyme | Danio rerio | |
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Release 2023.1 (January 2023)
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