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Literature summary extracted from

  • Mori, S.; Shibayama, K.; Wachino, J.; Arakawa, Y.
    Purification and molecular characterization of a novel diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase from Mycobacterium tuberculosis H37Rv (2010), Protein Expr. Purif., 69, 99-105.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.53 expressed in Escherichia coli BL21(DE3)pLysS cells Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.53 0.1
-
P1,P4-bis(5'-adenosyl) tetraphosphate in 50 mM HEPES-Na (pH 7.6), 1 mM MnCl2, at 37°C Mycobacterium tuberculosis
2.7.7.53 0.94
-
phosphate in 50 mM HEPES-Na (pH 7.6), 1 mM MnCl2, at 37°C Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.53 Ca2+ Rv2613c requires bivalent metal ions such as Ca2+ to exert its Ap4A phosphorylase activity (64% activity in the presence of 2 mM Ca2+) Mycobacterium tuberculosis
2.7.7.53 Co2+ Rv2613c requires bivalent metal ions such as Co2+ to exert its Ap4A phosphorylase activity (68% activity in the presence of 2 mM Co2+) Mycobacterium tuberculosis
2.7.7.53 Mg2+ Rv2613c requires bivalent metal ions such as Mg2+ to exert its Ap4A phosphorylase activity (34% activity in the presence of 2 mM Mg2+) Mycobacterium tuberculosis
2.7.7.53 Mn2+ Rv2613c requires bivalent metal ions such as Mn2+ to exert its Ap4A phosphorylase activity (100% activity in the presence of 2 mM Mn2+) Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.53 24683
-
4 * 24683, MALDI-TOF mass spectrometry Mycobacterium tuberculosis
2.7.7.53 100000
-
gel filtration Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.53 Mycobacterium tuberculosis P9WMK9
-
-
2.7.7.53 Mycobacterium tuberculosis H37Rv P9WMK9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.53 HisTrap HP column chromatography and Sephacryl S-200 gel filtration Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.53 3.01
-
crude enzyme, using P1,P4-bis(5'-adenosyl) tetraphosphate as substrate, in 50 mM HEPES-Na (pH 7.6), 1 mM MnCl2, at 37°C Mycobacterium tuberculosis
2.7.7.53 10.84
-
after 3.6fold purification, using P1,P4-bis(5'-adenosyl) tetraphosphate as substrate, in 50 mM HEPES-Na (pH 7.6), 1 mM MnCl2, at 37°C Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.53 additional information the enzyme shows no hydrolysis of AMP-NH2, and almost no activity with adenosine tetraphosphate, ATP, ADP-ribose, ADP-glucose, guanosine tetraphosphate, guanosine 5'-triphosphate, GDP-glucose, GDP-mannose, adenylyl imidodiphosphate, and guanylyl imidodiphosphate Mycobacterium tuberculosis ?
-
?
2.7.7.53 additional information the enzyme shows no hydrolysis of AMP-NH2, and almost no activity with adenosine tetraphosphate, ATP, ADP-ribose, ADP-glucose, guanosine tetraphosphate, guanosine 5'-triphosphate, GDP-glucose, GDP-mannose, adenylyl imidodiphosphate, and guanylyl imidodiphosphate Mycobacterium tuberculosis H37Rv ?
-
?
2.7.7.53 P1,P3-bis(5'-adenosyl) triphosphate + phosphate 19% activity compared to P1,P4-bis(5'-adenosyl) tetraphosphate Mycobacterium tuberculosis ADP
-
?
2.7.7.53 P1,P3-bis(5'-adenosyl) triphosphate + phosphate 19% activity compared to P1,P4-bis(5'-adenosyl) tetraphosphate Mycobacterium tuberculosis H37Rv ADP
-
?
2.7.7.53 P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate 100% activity Mycobacterium tuberculosis ATP + ADP
-
ir
2.7.7.53 P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate 100% activity Mycobacterium tuberculosis H37Rv ATP + ADP
-
ir
2.7.7.53 P1,P4-bis(5'-guanosyl) tetraphosphate + phosphate 107% activity compared to P1,P4-bis(5'-adenosyl) tetraphosphate Mycobacterium tuberculosis GTP + GDP
-
?
2.7.7.53 P1,P4-bis(5'-guanosyl) tetraphosphate + phosphate 107% activity compared to P1,P4-bis(5'-adenosyl) tetraphosphate Mycobacterium tuberculosis H37Rv GTP + GDP
-
?
2.7.7.53 P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate 106% activity compared to P1,P4-bis(5'-adenosyl) tetraphosphate Mycobacterium tuberculosis adenosine tetraphosphate + ADP
-
?
2.7.7.53 P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate 106% activity compared to P1,P4-bis(5'-adenosyl) tetraphosphate Mycobacterium tuberculosis H37Rv adenosine tetraphosphate + ADP
-
?
2.7.7.53 P1,P5-bis(5'-guanosyl) pentaphosphate + phosphate 106% activity compared to P1,P4-bis(5'-adenosyl) tetraphosphate Mycobacterium tuberculosis guanosine tetraphosphate + GDP
-
?
2.7.7.53 P1,P6-bis(5'-adenosyl) hexaphosphate + phosphate 25% activity compared to P1,P4-bis(5'-adenosyl) tetraphosphate Mycobacterium tuberculosis adenosine pentaphosphate + ADP
-
?
2.7.7.53 P1-(5'-adenosyl)P4-(5'-guanosyl) tetraphosphate + phosphate 100% activity compared to P1,P4-bis(5'-adenosyl) tetraphosphate Mycobacterium tuberculosis ATP + GDP
-
?
2.7.7.53 P1-(5'-adenosyl)P5-(5'-guanosyl) pentaphosphate + phosphate 76% activity compared to P1,P4-bis(5'-adenosyl) tetraphosphate Mycobacterium tuberculosis adenosine tetraphosphate + GDP
-
?

Subunits

EC Number Subunits Comment Organism
2.7.7.53 homotetramer 4 * 24683, MALDI-TOF mass spectrometry Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.7.7.53 diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase
-
Mycobacterium tuberculosis
2.7.7.53 Rv2613c the enzyme has Ap4A phosphorylase activity rather than Ap4A hydrolase activity Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.53 30
-
-
Mycobacterium tuberculosis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.53 65
-
the activity completely disappears after treatment at 65°C for 10 min Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.53 8
-
-
Mycobacterium tuberculosis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.53 7.4 10
-
Mycobacterium tuberculosis